B6.129S2-Usf1^{Gt(W233A07)Wrst}/J

Stock number: 016579
Research areas: Developmental Biology Research, Metabolism Research, Neurobiology Research

Description

These mice carry the ROSAbetageo+1 gene trap inserted into the mouse Usf1 (upstream transcription factor 1) gene.

Detailed description

These mice carry the ROSAbetageo+1 gene trap inserted into the mouse Usf1 (upstream transcription factor 1) gene. USF1 is involved with embryonic development, and is implicated as an antagonist of c-Myc protooncoprotein in the control of cellular proliferation. Suppression of Clock gene-regulated circadian period has also been noted in mice overexpressing Usf1. In humans, this gene has been linked to familiar combined hyperlipidemia (FCHL).

Development

The ROSAbetageo+1 gene trap vector (incorporating fused beta galactosidase and neomycin) was introduced to 129S2/SvPas-derived TBV2 embryonic stem (ES) cells to generate mutant cell lines. Clone W233A07 cells (German Gene Trap Consortium), carrying a vector insertion in the Usf1 gene, were used to generate mice which were backcrossed to C57BL/6J for more than 6 generations by the donating laboratory. The gene trap was localized to Chr1:17341444-173349273 bp (+ strand).

Allele

Symbol: Usf1^{Gt(W233A07)Wrst}
Name: gene trap W233A07, German Gene Trap Consortium
MGI accession ID: MGI:4336768
Generation method: Gene trapped
Attributes: Reporter; Null/Knockout
Marker symbol: Usf1
Marker name: upstream transcription factor 1
Chromosome: 1
Strain of origin: 129S2/SvPas
Molecular note: Trapped gene association determined by genome coordinate overlap and strand.