B6N;129S6-Nkx3-1^tm2Mms/J

Stock number: 016541
Research areas: Developmental Biology Research, Internal/Organ Research, Reproductive Biology Research, Research Tools

Description

In this Nkx3-1^lacZ mutant strain a β-galactosidase (lacZ) cassette disrupts the NK-3 transcription factor, locus 1 (Nkx3-1) gene, abolishing gene function. This strain may be useful for visualizing the differentiating prostate and the development of prostate tumors.

Detailed description

In this Nkx3-1^lacZ mutant strain a β-galactosidase (lacZ) cassette disrupts the NK-3 transcription factor, locus 1 (Nkx3-1) gene, abolishing gene function. Homozygous females are viable, fertile, and normal in size. The donating investigator reports some infertile homozygous males, with an increase in productivity when bred between 2-3 months of age. Expressed in the three glands of the adult prostate and in the bulbourethral gland (BUG) during prostate differentiation, Nkx3-1 is a putative prostate tumor suppressor. Homozygous males exhibit a moderate reduction in prostate ductal tip number compared to heterozygous littermates. Nkx3-1 expression is evident in prostate epithelium buds and ducts, the BUG, and urethral glands. This strain may be useful for visualizing the differentiating prostate and the development of prostate tumors.

Development

A targeting vector was designed to disrupt the NK-3 transcription factor, locus 1 (Nkx3-1) gene with a β-galactosidase (lacZ) cassette. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred with C57BL/6NTac females to establish a colony. These Nkx3-1^lacZ mice were maintained on a mixed B6N;129S6 background. Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation.

Allele

Symbol: Nkx3-1^tm2Mms
Name: targeted mutation 2, Michael M Shen
MGI accession ID: MGI:3038373
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Nkx3-1
Marker name: NK3 homeobox 1
Chromosome: 14
Strain of origin: 129S6/SvEvTac
Expressed genes: lacZ,beta-galactosidase,E. coli
Molecular note: The endogenous gene was disrupted by the insertion of cassette containing lacZ. Beta-galactosidase was expressed from this allele.