In this Nkx3-1lacZ mutant strain a β-galactosidase (lacZ) cassette disrupts the NK-3 transcription factor, locus 1 (Nkx3-1) gene, abolishing gene function. This strain may be useful for visualizing the differentiating prostate and the development of prostate tumors.
Michael Shen, Columbia University
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Nkx3-1 | NK3 homeobox 1 |
In this Nkx3-1lacZ mutant strain a β-galactosidase (lacZ) cassette disrupts the NK-3 transcription factor, locus 1 (Nkx3-1) gene, abolishing gene function. Homozygous females are viable, fertile, and normal in size. The donating investigator reports some infertile homozygous males, with an increase in productivity when bred between 2-3 months of age. Expressed in the three glands of the adult prostate and in the bulbourethral gland (BUG) during prostate differentiation, Nkx3-1 is a putative prostate tumor suppressor. Homozygous males exhibit a moderate reduction in prostate ductal tip number compared to heterozygous littermates. Nkx3-1 expression is evident in prostate epithelium buds and ducts, the BUG, and urethral glands. This strain may be useful for visualizing the differentiating prostate and the development of prostate tumors.
A targeting vector was designed to disrupt the NK-3 transcription factor, locus 1 (Nkx3-1) gene with a β-galactosidase (lacZ) cassette. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred with C57BL/6NTac females to establish a colony. These Nkx3-1lacZ mice were maintained on a mixed B6N;129S6 background. Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression |
Allele Name | targeted mutation 2, Michael M Shen |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Nkx3.1tm2(lacZ)Mms |
Gene Symbol and Name | Nkx3-1, NK3 homeobox 1 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 14 |
Molecular Note | The endogenous gene was disrupted by the insertion of cassette containing lacZ. Beta-galactosidase was expressed from this allele. |
Mutations Made By | Michael Shen, Columbia University |
When maintaining a live colony, homozygous females may be bred to heterozygous males. The donating investigator reports some infertile homozygous males, with an increase in productivity when bred between 2-3 months of age.
When using the B6N;129S6-Nkx3-1tm2Mms/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016541 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Nkx3-1<tm2Mms> |
Frozen Mouse Embryo | B6N;129S6-Nkx3-1<tm2Mms>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N;129S6-Nkx3-1<tm2Mms>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N;129S6-Nkx3-1<tm2Mms>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6N;129S6-Nkx3-1<tm2Mms>/J Frozen Embryo | $3373.50 |
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