B6;129-Kiz^tm1Cpl/J

Stock number: 016240
Research areas: Research Tools

Description

These floxed mutant mice (also referred to as Gm114^loxP) possess loxP sites flanking exons 5 and 6 of the Kiz gene. These mutant mice may be useful in generating conditional mutations for studies of germ cell biology and stem cell renewal and differentiation.

Detailed description

These mice possess loxP sites on either side of exons 5 and 6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 5 and 6 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in primordial germ cells (see Stock No. 008569 for example), this mutant mouse strain may be useful in studies of mammalian germ cell differentiation.

Development

A targeting vector containing an FRT-NEO-FRT-loxP cassette into intron 6, and a single loxP site was inserted upstream of exon 5. The construct was electroporated into 129S6/SvEvTac derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The donating investigator reported that the resulting chimeric animals were crossed to 129 (unspecified substrains) mice (see SNP note below). Upon arrival at The Jackson Laboratory, the mice were crossed to 129S1/SvImJ, (Stock No. 002448) at least once to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Ten markers throughout the genome suggested a significant C57BL/6 genetic contribution.

Allele

Symbol: Kiz^tm1Cpl
Name: targeted mutation 1, Blanche Capel
MGI accession ID: MGI:3797917
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Kiz
Marker name: kizuna centrosomal protein
Chromosome: 2
Strain of origin: 129S6/SvEvTac
Molecular note: A loxP site is place upstream of exon 5 and frt flanked neo cassette followed by another loxP site is placed downstream of exon 6 by homologous recombination.