These floxed mutant mice (also referred to as Gm114loxP) possess loxP sites flanking exons 5 and 6 of the Kiz gene. These mutant mice may be useful in generating conditional mutations for studies of germ cell biology and stem cell renewal and differentiation.
Blanche Capel, Duke University Medical Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Kiz | kizuna centrosomal protein |
These mice possess loxP sites on either side of exons 5 and 6 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 5 and 6 deleted in the cre-expressing tissue(s).
When bred to a strain with Cre recombinase expression in primordial germ cells (see Stock No. 008569 for example), this mutant mouse strain may be useful in studies of mammalian germ cell differentiation.
A targeting vector containing an FRT-NEO-FRT-loxP cassette into intron 6, and a single loxP site was inserted upstream of exon 5. The construct was electroporated into 129S6/SvEvTac derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The donating investigator reported that the resulting chimeric animals were crossed to 129 (unspecified substrains) mice (see SNP note below). Upon arrival at The Jackson Laboratory, the mice were crossed to 129S1/SvImJ, (Stock No. 002448) at least once to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Ten markers throughout the genome suggested a significant C57BL/6 genetic contribution.
Allele Name | targeted mutation 1, Blanche Capel |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Gm114loxP |
Gene Symbol and Name | Kiz, kizuna centrosomal protein |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 2 |
Molecular Note | A loxP site is place upstream of exon 5 and frt flanked neo cassette followed by another loxP site is placed downstream of exon 6 by homologous recombination. |
Mutations Made By | Blanche Capel, Duke University Medical Center |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6;129-Kiztm1Cpl/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016240 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Plk1s1<tm1Cpl> |
Frozen Mouse Embryo | B6;129-Kiz<tm1Cpl>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Kiz<tm1Cpl>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Kiz<tm1Cpl>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Kiz<tm1Cpl>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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