B6(Cg)-Ciao3^tm1.1Fsl/J

Stock number: 016235
Product name: Iop1 f
Research areas: Metabolism Research, Research Tools

Description

These floxed mutant mice (also referred to as Iop1 f) possess loxP sites flanking exon 1 of the Ciao3 (also known as Narfl) gene. This strain may be useful for generating conditional mutations in applications related to studies of the cytoplasmic iron-sulfur complex assembly pathway.

Detailed description

These Iop1^flox mutant mice possess loxP sites flanking exon 1 of the nuclear prelamin A recognition factor-like (Ciao3, also known as Narfl or Iop1) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. IOP1 is required for cytosolic iron-sulfur protein assembly pathways. Iron-sulfur proteins are involved in the Krebs Cycle, oxidative phosphorylation, translation, iron regulatory pathways, and purine metabolism. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 1 deleted in the cre-expressing tissues. This strain may be useful for conditional deletion of IOP1 to study the cytoplasmic iron-sulfur complex assembly pathway.

Development

A targeting vector was designed to insert a loxP site upstream of exon 1 followed by an frt-flanked neomycin resistance (neo) cassette, and a second loxP site downstream of exon 1 of the nuclear prelamin A recognition factor-like (Ciao3, also known as Narfl or Iop1) gene. The construct was electroporated into C57BL/6-derived EAP6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts and resulting chimeric mice were bred to B6(Cg)-Tyr^c-2J/J (Stock No. 000058). Offspring, heterozygous for this allele, were bred with C57BL/6-Tg(ACTBFlpe)2Arte mice to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. The donating investigator reported that the resulting mice were then backcrossed for at least 5 generations to C57BL/6 mice (see SNP note below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Allele

Symbol: Ciao3^tm1.1Fsl
Name: targeted mutation 1.1, Frank Lee
MGI accession ID: MGI:4946831
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Ciao3
Marker name: cytosolic iron-sulfur assembly component 3
Chromosome: 17
Strain of origin: C57BL/6
Molecular note: A targeting vector was designed to insert a loxP site upstream of exon 1 followed by an frt-flanked neomycin resistance (neo) cassette, and a second loxP site downstream of exon 1 of the nuclear prelamin A recognition factor-like (Narfl or Iop1) gene. Flp-mediated recombination removed the neo cassette.