Overview

Also Known As:Id2-CreER^T2

This Id2-CreERT2 knock-in allele was designed to both abolish Id2 gene function and direct CreER^T2 fusion protein expression from the endogenous Id2 promoter/enhancer elements. Cre-ERT2 fusion gene activity is inducible by tamoxifen administration.

Donating Investigator

Yuan Zhuang, Duke University Medical Center

Genetic overview

Genetic Background	Generation

Id2^{tm1.1(cre/ERT2)Blh}

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing, Inducible)	Id2	inhibitor of DNA binding 2

View Genetics

Research Applications

Developmental Biology Research
Hematological Research
Research Tools
Internal/Organ Research
Immunology, Inflammation and Autoimmunity Research
Endocrine Deficiency Research
Reproductive Biology Research
Cell Biology Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

The Id2-CreERT2 knockin allele was designed to both abolish inhibitor of DNA binding 2 (Id2) gene function and express CreER^T2 fusion protein from the Id2 promoter/enhancer elements. Cre-ERT2 fusion gene activity is inducible and can be observed following tamoxifen administration. As such, when Id2-CreERT2 knockin mice are bred with mice containing loxP-flanked sequences, tamoxifen-inducible Cre-mediated recombination will result in deletion of the floxed sequences in the Id2-expressing cells of the offspring.

No mRNA or protein expression from the Id2-CreERT2 allele is observed. The donating investigator reports that homozygous mice are runted with defective lung alveolarization. Other organ systems have not been evaluated. However, Id2-CreERT2 homozygotes may be expected to exhibit the same phenotype as mice homozygous for other null mutations of this gene (including postnatal lethality and defects of the immune system, digestive tract, kidneys, adipose tissue and mammary gland development).Heterozygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The donating investigator also reports tamoxifen-inducible Cre recombinase activity recapitulates the endogenous Id2 expression pattern. Following tamoxifen administration, Cre recombinase activity recapitulates the epithelial expression of the endogenous gene (distal tip lung epithelial multipotent precursor cells of the developing lung throughout branching morphogenesis up to embryonic day (E)16.5). Additionally, the Cre recombinase activity in immune cells (during lymphopoiesis, hematopoiesis, or adaptive immune responses) was not directly assessed by the donating investigator. Cre recombinase activity is not observed in lung tissue prior to tamoxifen treatment(other systems not examined).

Of note, the same group designed an identically-targeted Id2-eGFP knockin mouse line to express an enhanced green fluorescent protein (rather than Cre-ERT2 fusion protein) from the Id2 promoter/enhancer elements. That Id2-eGFP knockin mouse line is available as Stock No. 016224.

The Cre-ERT2 fusion protein consists of Cre recombinase fused to a triple mutant form of the human estrogen receptor which does not bind its natural ligand (17β-estradiol) at physiological concentrations but will bind the synthetic estrogen receptor ligands 4-hydroxytamoxifen (OHT or tamoxifen) and, with lesser sensitivity, ICI 182780. Restricted to the cytoplasm, Cre-ERT2 can only gain access to the nuclear compartment after exposure to tamoxifen. To counteract the mixed estrogen agonist effects of tamoxifen injections, which can result in late fetal abortions in pregnant mice, progesterone may be coadministered.

Development

The Id2-CreERT2 knockin mutation was created by the laboratory of Dr. Brigid LM Hogan (Duke University Medical Center). A targeting vector was designed to insert a CreER^T2 fusion gene (followed by an frt-flanked PGK-neo cassette) into the translation initiation codon of the inhibitor of DNA binding 2 locus (Id2); replacing most of exon 1 except the final 25 nucleotides. The CreER^T2 fusion gene (Cre-ER^T2) is Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain. The targeting construct was electroporated into 129SvEv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6NCrl mice to originate the colony. Mutant mice were bred with ROSA26-FLPe mice (on an uncharacterized genetic background) to remove the PGK-neo selection cassette. These Id2-CreERT2 knockin mice were subsequently bred to C57BL/6NCrl mice for 5-8 generations (and the ROSA26-FLPe was removed). Next, heterozygous mutant mice were sent to Dr. Yuan Zhang (Duke University) where they were backcrossed to C57BL/6J mice (see SNP note below) for at least one additional generation prior to sending to The Jackson Laboratory Repository. Upon arrival, Id2-CreERT2 knockin mice were bred with C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the Id2-CreERT2 knockin colony. During backcrossing, the donating investigator reports that the Y chromosome was fixed to the C57BL/6J genetic background.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Expression Data

Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	Following tamoxifen administration, Cre recombinase activity recapitulates the epithelial expression of the endogenous gene (distal tip lung epithelial multipotent precursor cells of the developing lung throughout branching morphogenesis up to embryonic day (E)16.5).

Control Suggestions

Wild-type from the colony

Approximate Controls

005304 C57BL/6NJ

Additional Information

Considerations for Choosing Controls

Selected References

Rawlins EL; Clark CP; Xue Y; Hogan BL. 2009. The Id2+ distal tip lung epithelium contains individual multipotent embryonic progenitor cells. Development 136(22):3741-5PubMed: 19855016 MGI: J:154450

View All References

Genetics

Id2^{tm1.1(cre/ERT2)Blh}

Allele Symbol: Id2^{tm1.1(cre/ERT2)Blh}

Allele Name	targeted mutation 1.1, Brigid L Hogan
Allele Type	Targeted (Recombinase-expressing, Inducible)
Allele Synonym(s)	Id2^{tm1(CRE/ESR1)Blh}; Id2-CreER^T2
Gene Symbol and Name	Id2, inhibitor of DNA binding 2
Gene Synonym(s)
Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	Following tamoxifen administration, Cre recombinase activity recapitulates the epithelial expression of the endogenous gene (distal tip lung epithelial multipotent precursor cells of the developing lung throughout branching morphogenesis up to embryonic day (E)16.5).
Strain of Origin	129S/SvEv
Chromosome	12
Molecular Note	The targeting vector contained an cre/ERT2 cassette inserted at the translation initiation codon of the Id2 locus, replacing most of exon 1, except for the final 25 nucleotides. The cre cassette was followed by a frt-PGK-neo-frt cassette, which was removed by flp-mediated excision after germline transmission.
Mutations Made By	Brigid Hogan, Duke University Medical Center

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Developmental Biology Research
- Internal/Organ Defects
  - hematopoietic defects
  - Lymphoid Tissue Defects
  - lung
- Perinatal Lethality
  - Homozygous
- Lymphoid Tissue Defects
  - hematopoietic defects
- Mesodermal Defects
Hematological Research
- Hematopoietic Defects
- Immunological Defects
Research Tools
- Immunology, Inflammation and Autoimmunity Research
  - specific T cell deficiency
  - NK Cell Deficiency
  - T cell deficiency
- Cardiovascular Research
  - Cre-lox System
- Reproductive Biology Research
  - Cre-lox System
  - male germ cells
- Cre-lox System
  - Cre Recombinase Expression: Inducible
  - Cre Recombinase Expression
- Developmental Biology Research
  - Cre-lox System
- Genetics Research
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers: Cre-lox System
  - Mutagenesis and Transgenesis: transcriptional activation
- Hematological Research
Internal/Organ Research
- Lung Defects
- Lymphoid Tissue Defects
Immunology, Inflammation and Autoimmunity Research
- Lymphoid Tissue Defects
  - hematopoietic development
- Growth Factors/Receptors/Cytokines
- Immunodeficiency
  - NK Cell and Cd8 T Cell defects
  - NK Cell Deficiency
  - T cell deficiency
  - specific T cell deficiency
- Intracellular Signaling Molecules
Endocrine Deficiency Research
- Mammary Gland Defects
Reproductive Biology Research
- Developmental Defects Affecting Gonads
  - males only
- Fertility Defects
  - males only
Cell Biology Research
- Signal Transduction
- Transcriptional Regulation

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Id2^{tm1.1(cre/ERT2)Blh}/Id2⁺
involves: 129S/SvEv * C57BL/6

mammalian phenotype

no phenotypic analysis
- Normal - no analysis details are provided; mice are used for expression studies only

References

Rawlins EL; Clark CP; Xue Y; Hogan BL. 2009. The Id2+ distal tip lung epithelium contains individual multipotent embryonic progenitor cells. Development 136(22):3741-5PubMed: 19855016 MGI: J:154450

Additional - Id2^{tm1.1(cre/ERT2)Blh} related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Id2
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings or to C57BL/6NJ inbred mice (Stock No. 005304). Breeding homozygous mice on a C57BL/6 genetic background together may be expected to result in perinatal lethality with nearly full penetrance. This perinatal lethality is significantly diminished if maintaining homozygous mice on a mixed genetic background.
- Additional Breeding and Husbandry Support
Mating System
- Heterozygote x +/+ sibling
Citation
When using the Id2-CreER^T2 mouse strain in a publication, please cite the originating article(s) and include JAX stock #016222 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous or wildtype for Id2<tm1.1(cre/ERT2)Blh>

Related Products and Services

Frozen Mouse Embryo	B6.129S(Cg)-Id2<tm1.1(cre/ERT2)Blh>/ZhuJ Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6.129S(Cg)-Id2<tm1.1(cre/ERT2)Blh>/ZhuJ Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6.129S(Cg)-Id2<tm1.1(cre/ERT2)Blh>/ZhuJ Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6.129S(Cg)-Id2<tm1.1(cre/ERT2)Blh>/ZhuJ Frozen Embryo	$3373.50

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Also Known As:Id2-CreERT2

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Id2tm1.1(cre/ERT2)Blh

Allele Symbol: Id2tm1.1(cre/ERT2)Blh

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Id2tm1.1(cre/ERT2)Blh/Id2+involves: 129S/SvEv * C57BL/6

mammalian phenotype

References

Additional - Id2tm1.1(cre/ERT2)Blh related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As:Id2-CreER^T2

Id2^{tm1.1(cre/ERT2)Blh}

Allele Symbol: Id2^{tm1.1(cre/ERT2)Blh}

Genotype: Id2^{tm1.1(cre/ERT2)Blh}/Id2⁺
involves: 129S/SvEv * C57BL/6

Additional - Id2^{tm1.1(cre/ERT2)Blh} related