B6;129-Ip6k2^tm1Dlin/J

Stock number: 016221
Product name: IHPK2-
Research areas: Apoptosis Research, Cancer Research, Cell Biology Research

Description

In this knockout strain the first 202 nucleotides of exon 2 of the inositol hexaphosphate kinase 2 (Ip6k2) gene is disrupted. These mice may be useful for studying the mechanisms of IP6K2-dependent tumorigenesis.

Detailed description

In this strain, a floxed-neo cassette replaces the first 202 nucleotides of exon 2 of the inositol hexaphosphate kinase 2 (Ip6k2) gene, abolishing gene expression. Homozygotes are viable, fertile, and normal in size. IP6K2 suppresses growth and increases apoptosis during cell stress by catalyzing the synthesis of inositol polyphosphates. These compounds are involved in the regulation of telomere length, protein phosphorylation, and cell growth and movement. After chronic exposure to the carcinogen 4-nitroquinoline 1-oxide (4-NQO) these mice exhibit a four-fold increase in the incidence of invasive squamous cell carcinoma (SCC) formation in the tongue, oral cavity, and esophagus. They also display relative resistance to ionizing radiation and exhibit increased survival following total body irradiation. Cells from these mice are relatively resistant to growth-suppression by interferon-β (IFN-β). These mice may be useful for studying the mechanisms of IP6K2-dependent tumorigenesis.

Development

A targeting vector was designed to replace the first 202 nucleotides of exon 2 of the inositol hexaphosphate kinase 2 (Ip6k2) gene with a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into 129SvJ-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6J females to generate a colony of IHPK2- mice. These mice were maintained on a mixed B6;129S background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Ip6k2^tm1Dlin
Name: targeted mutation 1, D J Lindner
MGI accession ID: MGI:4354090
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: IHPK2^-
Marker symbol: Ip6k2
Marker name: inositol hexaphosphate kinase 2
Marker synonyms: 1500005N04Rik; IHPK2; InsP6K2; Pius
Chromosome: 9
Strain of origin: 129X1/SvJ
Molecular note: The first 202 bp of exon 2 were replaced with a neo cassette. The absence of protein expression was confirmed by western blot analysis on tail snip extracts.