In this knockout strain the first 202 nucleotides of exon 2 of the inositol hexaphosphate kinase 2 (Ip6k2) gene is disrupted. These mice may be useful for studying the mechanisms of IP6K2-dependent tumorigenesis.
Daniel Lindner, Cleveland Clinic Main Campus
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Ip6k2 | inositol hexaphosphate kinase 2 |
In this strain, a floxed-neo cassette replaces the first 202 nucleotides of exon 2 of the inositol hexaphosphate kinase 2 (Ip6k2) gene, abolishing gene expression. Homozygotes are viable, fertile, and normal in size. IP6K2 suppresses growth and increases apoptosis during cell stress by catalyzing the synthesis of inositol polyphosphates. These compounds are involved in the regulation of telomere length, protein phosphorylation, and cell growth and movement. After chronic exposure to the carcinogen 4-nitroquinoline 1-oxide (4-NQO) these mice exhibit a four-fold increase in the incidence of invasive squamous cell carcinoma (SCC) formation in the tongue, oral cavity, and esophagus. They also display relative resistance to ionizing radiation and exhibit increased survival following total body irradiation. Cells from these mice are relatively resistant to growth-suppression by interferon-β (IFN-β). These mice may be useful for studying the mechanisms of IP6K2-dependent tumorigenesis.
A targeting vector was designed to replace the first 202 nucleotides of exon 2 of the inositol hexaphosphate kinase 2 (Ip6k2) gene with a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into 129SvJ-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6J females to generate a colony of IHPK2- mice. These mice were maintained on a mixed B6;129S background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, D J Lindner |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | IHPK2- |
Gene Symbol and Name | Ip6k2, inositol hexaphosphate kinase 2 |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 9 |
Molecular Note | The first 202 bp of exon 2 were replaced with a neo cassette. The absence of protein expression was confirmed by western blot analysis on tail snip extracts. |
Mutations Made By | Daniel Lindner, Cleveland Clinic Main Campus |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6;129-Ip6k2tm1Dlin/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016221 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Ip6k2<tm1Dlin> |
Frozen Mouse Embryo | B6;129-Ip6k2<tm1Dlin>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Ip6k2<tm1Dlin>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Ip6k2<tm1Dlin>/J | $3373.50 |
Frozen Mouse Embryo | B6;129-Ip6k2<tm1Dlin>/J | $3373.50 |
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