These NS4 mutant mice possess loxP sites flanking exon 20 (alternative splice site 4) of the neurexin III (Nrxn3) gene, and a mutated exon 20 splice acceptor sequence. This strain may be useful for studying neuronal synapses related to alcoholism, dependence, and addiction behaviors.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Modified isoform(s)) | Nrxn3 | neurexin III |
These NS4 mutant mice possess loxP sites flanking exon 20 (alternative splice site 4) of the neurexin III (Nrxn3) gene, and a mutated exon 20 splice acceptor sequence. Mice that are homozygous for this allele are viable, fertile, and normal in size. NRXN3, an intercellular cell signaling protein, is expressed in neurons involved in addictive behaviors and is associated with nicotine and opioid dependence and substance abuse. Nrxn3 has two distinct promoters and five alternative splice sites. These Nrxn3 mice always incorporate alternative splice site 4 in the transcript. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have this mutated exon 20 deleted in the cre-expressing tissues. This strain may be useful for studying neuronal synapses related to alcoholism, dependence, and addiction behaviors.
A targeting vector was designed to insert a loxP site upstream of exon 20 (alternative splice site 4) followed by a frt-flanked neomycin resistance (neo) cassette, and a second loxP site downstream of exon 20 of the neurexin III (Nrxn3) gene. The splice acceptor sequence of exon 20 (TGTTTTTTAAACTTTAAAG) was substituted with a generic one (TGTTTTTTCGTCTTCCTAG) to make the exon always incorporated in the transcript. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl<+>-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred. Offspring, were bred with Flp transgenic mice on a mixed C57BL/6:129 background to delete the neo cassette. The resulting progeny were crossed to remove the Flp-expressing transgene. These NS4 mice were maintained on a mixed background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 4.1, Thomas C Sudhof |
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Allele Type | Targeted (Modified isoform(s)) |
Allele Synonym(s) | Nrx3ss4+; NS4 |
Gene Symbol and Name | Nrxn3, neurexin III |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 12 |
Molecular Note | A targeting vector was designed to insert a loxP site upstream of exon 20 (alternative splice site 4) followed by a frt-flanked neomycin resistance (neo) cassette, and a second loxP site downstream of exon 20. The less common splice acceptor sequence of exon 20 (TGTTTTTTAAACTTTAAAG) was substituted with a much more common one (TGTTTTTTCGTCTTCCTAG) to make the exon always incorporated in the transcript. |
When maintaining a live colony, homozygous or heterozygous mice may be bred together. The donating investigator reports 30% of animals don't produce offspring.
When using the NS4 mouse strain in a publication, please cite the originating article(s) and include JAX stock #016194 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Nrxn3<tm4.1Sud> |
Frozen Mouse Embryo | B6;129-Nrxn3<tm4.1Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Nrxn3<tm4.1Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Nrxn3<tm4.1Sud>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Nrxn3<tm4.1Sud>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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