This strain carries the Clock (circadian locomotor output cycles kaput) delta 19 ENU mutation on a BALB/cJ genetic background. This strain may be useful in studies of circadian rhythm.
Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr
This strain carries the delta 19 Clock (circadian locomotor output cycles kaput) mutation on a BALB/cJ genetic background. As compared with the original C57BL/6 background see Stock No. 002923), the circadian phenotype of BALB/c animals is suppressed. Heterozygotes and homozygotes are viable and fertile.
ENU-mutagenesis of male C57BL/6J mice created an A to T transversion at the third base position of the 5' splice donor site of intron 19. Exon 19 is missing from Clock mRNA, causing a 51 amino acid deletion within the glutamine-rich region of the protein's C terminus (amino acids 514-564). Mice with this Clockdelta19 mutation (also called Clockmut or Clockm1Jt) were originally maintained on the C57BL/6J genetic background (see Stock No. 002923). This Clockdelta19 strain has been backcrossed to BALB/cJ for more than 10 generations by the donating laboratory.
|Allele Type||Chemically induced (ENU)|
|Allele Synonym(s)||Ck; Clockdelta19; Clockmut|
|Gene Symbol and Name||Clock, circadian locomotor output cycles kaput|
|Gene Synonym(s)||5330400M04Rik; 5330400M04Rik; KAT13D; RIKEN cDNA 5330400M04 gene; bHLHe8; mKIAA0334|
|Strain of Origin||C57BL/6J|
|General Note||Phenotypic Similarity to Human Syndrome: Delayed Sleep Phase Syndrome J:125037. |
Phenotypic Similarity to Human Syndrome: Bipolar disorder J:166742.
Phenotypic Similarity to Human Syndrome: Diabetes Mellitus J:162641.
|Molecular Note||ENU mutagenesis caused an A to T transversion at the third base position of the 5' splice donor site of intron 19. RT-PCR using primers for exon 15 and exon 21 detected shorter transcripts and no wild-type transcript in hypothalamus of homozygous mutant mice. Sequence analysis revealed that these shorter transcripts are missing exon 19. The authors predict the protein will be missing a 51 amino acid region within the carboxy terminus.|
|Mutations Made By|| |
Dr. Joseph Takahashi, Univ Texas Southwestern Medical Ctr
|Please inquire about possible genotypes.|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
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