C.B6-Clock^m1Jt/J

Stock number: 016175
Research areas: Neurobiology Research, Reproductive Biology Research

Description

This strain carries the Clock (circadian locomotor output cycles kaput) delta 19 ENU mutation on a BALB/cJ genetic background. This strain may be useful in studies of circadian rhythm.

Detailed description

This strain carries the delta 19 Clock (circadian locomotor output cycles kaput) mutation on a BALB/cJ genetic background. As compared with the original C57BL/6 background see Stock No. 002923), the circadian phenotype of BALB/c animals is suppressed. Heterozygotes and homozygotes are viable and fertile.

Development

ENU-mutagenesis of male C57BL/6J mice created an A to T transversion at the third base position of the 5' splice donor site of intron 19. Exon 19 is missing from Clock mRNA, causing a 51 amino acid deletion within the glutamine-rich region of the protein's C terminus (amino acids 514-564). Mice with this Clock^delta19 mutation (also called Clock^mut or Clock^m1Jt) were originally maintained on the C57BL/6J genetic background (see Stock No. 002923). This Clock^delta19 strain has been backcrossed to BALB/cJ for more than 10 generations by the donating laboratory.

Allele

Symbol: Clock^m1Jt
Name: Clock
MGI accession ID: MGI:1861634
Generation method: Chemically induced (ENU)
Marker symbol: Clock
Marker name: circadian locomotor output cycles kaput
Chromosome: 5
Strain of origin: C57BL/6J
Molecular note: ENU mutagenesis caused an A to T transversion at the third base position of the 5' splice donor site of intron 19. RT-PCR using primers for exon 15 and exon 21 detected shorter transcripts and no wild-type transcript in hypothalamus of homozygous mutant mice. Sequence analysis revealed that these shorter transcripts are missing exon 19. The authors predict the protein will be missing a 51 amino acid region within the carboxy terminus.