This is a Mast2 (microtubule associated serine/threonine kinase 2) gene trap strain. No circadian phenotype has been observed in these mice.
Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Gene trapped (Not Applicable) | Mast2 | microtubule associated serine/threonine kinase 2 |
No phenotype has been reported for this Mast2 (microtubule associated serine/threonine kinase 2) gene trap strain. The circadian phenotype of these mice appears to be normal. Homozygotes are viable and fertile. It is not known whether the gene trap insertion affects expression of the targeted gene.
The pT1βgeo gene trap vector (incorporating fused beta galactosidase and neomycin) was introduced to 129S2/SvPas-derived TBV2 embryonic stem (ES) cells to generate mutant cell lines. Clone A061F01 cells (German Gene Trap Consortium), carrying a vector insertion in the Mast2 gene, were used to generate mice. The donating investigator reported that these mice were backcrossed to C57BL/6J for 5 generations (see SNP note below). The gene trap was localized to Chr4:116025577-116103147 bp (- strand).
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. 3 of the 27 markers, on Chromosomes 11 and 16, were found to be segregating with 129 suggesting an incomplete backcross.
Allele Name | gene trap A061F01, German Gene Trap Consortium |
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Allele Type | Gene trapped (Not Applicable) |
Allele Synonym(s) | |
Gene Symbol and Name | Mast2, microtubule associated serine/threonine kinase 2 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 4 |
General Note | No phenotype has been reported for this Mast2 (microtubule associated serine/threonine kinase 2) gene trap strain. The circadian phenotype of these mice appears to be normal. Homozygotes are viable and fertile. It is not known whether the gene trap insertion affects expression of the targeted gene. |
Molecular Note | Clone A061F01 cells (German Gene Trap Consortium), carrying a pT1betageo vector insertion in the Mast2 gene, were used to generate mice. The gene trap was localized to Chr4:116025577-116103147 bp (- strand). It is not known whether the gene trap insertion affects expression of the targeted gene. |
Mutations Made By | Wolfgang Wurst, HelmholtzZentrum Muenchen |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the B6;129S2-Mast2Gt(A061F01)Wrst/JtJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #016173 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Mast2<Gt(A061F01)Wrst> |
Frozen Mouse Embryo | B6;129S2-Mast2<Gt(A061F01)Wrst>/JtJ | $2595.00 |
Frozen Mouse Embryo | B6;129S2-Mast2<Gt(A061F01)Wrst>/JtJ | $2595.00 |
Frozen Mouse Embryo | B6;129S2-Mast2<Gt(A061F01)Wrst>/JtJ | $3373.50 |
Frozen Mouse Embryo | B6;129S2-Mast2<Gt(A061F01)Wrst>/JtJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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