B6;129S2-Mast2^{Gt(A061F01)Wrst}/JtJ

Stock number: 016173

Description

This is a Mast2 (microtubule associated serine/threonine kinase 2) gene trap strain. No circadian phenotype has been observed in these mice.

Detailed description

No phenotype has been reported for this Mast2 (microtubule associated serine/threonine kinase 2) gene trap strain. The circadian phenotype of these mice appears to be normal. Homozygotes are viable and fertile. It is not known whether the gene trap insertion affects expression of the targeted gene.

Development

The pT1βgeo gene trap vector (incorporating fused beta galactosidase and neomycin) was introduced to 129S2/SvPas-derived TBV2 embryonic stem (ES) cells to generate mutant cell lines. Clone A061F01 cells (German Gene Trap Consortium), carrying a vector insertion in the Mast2 gene, were used to generate mice. The donating investigator reported that these mice were backcrossed to C57BL/6J for 5 generations (see SNP note below). The gene trap was localized to Chr4:116025577-116103147 bp (- strand).

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. 3 of the 27 markers, on Chromosomes 11 and 16, were found to be segregating with 129 suggesting an incomplete backcross.

Allele

Symbol: Mast2^{Gt(A061F01)Wrst}
Name: gene trap A061F01, German Gene Trap Consortium
MGI accession ID: MGI:4337715
Generation method: Gene trapped
Attributes: Not Applicable
Marker symbol: Mast2
Marker name: microtubule associated serine/threonine kinase 2
Chromosome: 4
Strain of origin: 129S2/SvPas
Molecular note: Clone A061F01 cells (German Gene Trap Consortium), carrying a pT1betageo vector insertion in the Mast2 gene, were used to generate mice. The gene trap was localized to Chr4:116025577-116103147 bp (- strand). It is not known whether the gene trap insertion affects expression of the targeted gene.
General note: No phenotype has been reported for this Mast2 (microtubule associated serine/threonine kinase 2) gene trap strain. The circadian phenotype of these mice appears to be normal. Homozygotes are viable and fertile. It is not known whether the gene trap insertion affects expression of the targeted gene.