The mouse Per1 (period homolog 1 (Drosophila)) promoter drives in vivo and in vitro expression of luciferase in a pattern recapitulating that of circadian rhythms in this transgenic strain. These mice may be useful in studies of circadian and photic regulation of the mammalian circadian oscillator.
Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
In this transgenic strain, the mouse Per1 (period homolog 1 (Drosophila)) promoter drives in vivo and in vitro expression of luciferase in a pattern recapitulating that of circadian rhythms in the suprachiasmatic nuclei (SCN). Long-term circadian rhythms of luminescence are also seen in SCN slice culture. Pulses of light rapidly induce high luciferase mRNA expression in the SCN that, unlike endogenous Per1 mRNA levels, are sustained and do not return to baseline. This P1L141 line exhibits highly specific SCN expression. Luciferase riboprobe hybridization signal intensities indicate that among the various transgenic lines made by this research group, copy number does not correlate well with expression levels (possibly due to integration site differences). This strain is reported to carry approximately 10 copies of the transgene, but copy number analysis performed at the Jackson laboratory indicates that transgenic line 128 (see Stock No. 016165) is of higher luciferase copy number than transgenic line 141 (see Stock No. 016166).
6.75 kb of the mouse Per1 promoter (including exon 1, intron 1 and the untranslated portion of exon 2 up to the initiaton ATG) drives expression of luciferase in this transgenic strain. The initiator ATG of both Per1 and luciferase were engineered to coincide. The vector was introduced to CD1 embryos. This strain was backcrossed to C57BL/6J for 10 generations by the donating laboratory. This strain is reported to carry approximately 10 copies of the transgene, but copy number analysis performed at the Jackson laboratory indicates that transgenic line 128 (see Stock No. 016165) is of higher luciferase copy number than transgenic line 141 (see Stock No. 016166).
Expressed Gene | luc, luciferase, firefly |
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Site of Expression | Luciferase is expressed in a pattern recapitulating that of circadian rhythms in the suprachiasmatic nuclei (SCN). |
Allele Name | transgene insertion 141, Joseph S Takahashi |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Per1 Luc 141 |
Gene Symbol and Name | Tg(Per1-luc)141Jt, transgene insertion 141, Joseph S Takahashi |
Gene Synonym(s) | |
Promoter | Per1, period circadian clock 1, mouse, laboratory |
Expressed Gene | luc, luciferase, firefly |
Site of Expression | Luciferase is expressed in a pattern recapitulating that of circadian rhythms in the suprachiasmatic nuclei (SCN). |
Strain of Origin | CD-1 |
Chromosome | UN |
Molecular Note | 6.75 kb of the mouse Per1 promoter (including exon 1, intron 1 and the untranslated portion of exon 2 up to the initiaton ATG) drives expression of luciferase. |
Mutations Made By | Dr. Joseph Takahashi, Univ Texas Southwestern Medical Ctr |
When maintained as a live colony, hemizygotes may be bred with wildtype mice.
When using the B6.Cg-Tg(Per1-luc)141Jt/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016166 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Per1-luc)141Jt |
Frozen Mouse Embryo | B6.Cg-Tg(Per1-luc)141Jt/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Per1-luc)141Jt/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Per1-luc)141Jt/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Per1-luc)141Jt/J Frozen Embryo | $3373.50 |
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