Overview

These Adar^flox mutant mice may be useful in organ specific studies involving embryogenesis and stress induced apoptosis.

Donating Investigator

Kazuko Nishikura, The Wistar Institute

Genetic overview

Genetic Background	Generation

Adar^tm1Knk

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Adar	adenosine deaminase, RNA-specific

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Research Applications

Research Tools

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Details

Detailed Description

Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Widespread excision of the floxed fragment in homozygous mutants during embryogenesis results in embryonic lethality between E11 and E12.5. Widespread apoptosis is detected in tissues at E10.5 to E11.5, however, embryos appear grossly normal with the exception of occasional pallor and reduced size. This mutant strain is useful in organ specific studies involving embryogenesis and stress induced apoptosis.

Heterozygote: Normal

Development

A targeting vector containing a 12Kb fragment of mouse genomic Adar DNA and a loxP-flanked PGKneo cassette was placed within intron 11. A third loxP site was inserted within the 3'-UTR. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1 derived R1 embryonic stem (ES) cells. Transient Cre expression in targeted cells excised the neo cassette leaving loxP sites in intron 11 and downstream of exon 15. Correctly targeted ES cells were injected into blastocysts. The donating investigator reported that the resulting chimeric animals were crossed to C57BL/6 and offspring were backcrossed to C57BL/6J for 10 generations (see SNP note below). Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Wang Q; Miyakoda M; Yang W; Khillan J; Stachura DL; Weiss MJ; Nishikura K. 2004. Stress-induced apoptosis associated with null mutation of ADAR1 RNA editing deaminase gene. J Biol Chem 279(6):4952-61PubMed: 14613934 MGI: J:87714

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Genetics

Adar^tm1Knk

Allele Symbol: Adar^tm1Knk

Allele Name	targeted mutation 1, Kazuko Nishikura
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	ADAR1^flox
Gene Symbol and Name	Adar, adenosine deaminase, RNA-specific
Gene Synonym(s)
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	3
Molecular Note	Sequence encompassing exons 12 through 15 was flanked by single loxP sites in intron 11 and downstream of exon 15.
Mutations Made By	Kazuko Nishikura, The Wistar Institute

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - loxP-flanked Sequences
- Apoptosis Research
- Developmental Biology Research
  - Cre-lox System

Mammalian Phenotype Terms by Genotype

References

Wang Q; Miyakoda M; Yang W; Khillan J; Stachura DL; Weiss MJ; Nishikura K. 2004. Stress-induced apoptosis associated with null mutation of ADAR1 RNA editing deaminase gene. J Biol Chem 279(6):4952-61PubMed: 14613934 MGI: J:87714

Additional - Adar^tm1Knk related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:AdarMm
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these mice are bred as homozygotes.
- Additional Breeding and Husbandry Support
Citation
When using the B6.129-Adar^tm1Knk/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #34619 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

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Questions about Terms of Use

Licensing Information

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Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Adartm1Knk

Allele Symbol: Adartm1Knk

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Adartm1Knk related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Adar^tm1Knk

Allele Symbol: Adar^tm1Knk

Additional - Adar^tm1Knk related