NOD.Cg-Prkdc^scid Alox15^tm1Fun/NadlJ

Stock number: 016148
Product name: NOD.SCID, Alox15
Research areas: Cardiovascular Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools, Virology Research

Description

These mutant mice, commonly referred to as NOD.scid12LOKO, combine the features of the NOD/ShiLtJ background, the severe combined immune deficiency mutation (scid), and Alox15 deficiency This mutant mouse strain may be useful for adoptive transfer studies to further investigate the role of Alox15 in autoimmunity.

Detailed description

Mice homozygous for Alox15^tm1Fun and Prkdc^scid, commonly referred to as NOD.scid12LOKO, are viable and fertile. NOD.scid12LOKO mice injected with NOD/ShiLtJ, Stock No. 001976, splenocytes do not develop diabetes. This strain may be useful in adoptive transfer studies.

Development

Alox15^tm1Fun from Stock No. 014163 - NOD.129S2(B6)-Alox15^tm1Fun/NadlJ was crossed to Stock No. 001303 - NOD.CB17-Prkdc^scid/J. Progeny resulting from this cross were intercrossed to homozygosity. In 2011, the Type 1 Diabetes Resource received this strain at generation N6F6.

Allele

Symbol: Prkdc^scid
Name: severe combined immunodeficiency
MGI accession ID: MGI:1857113
Generation method: Spontaneous
Marker symbol: Prkdc
Marker name: protein kinase, DNA activated, catalytic polypeptide
Chromosome: 16
Strain of origin: C.BKa-Igh^b/Icr
Site of expression: T and B lymphocytes.
Molecular note: A T-to-A transversion point mutation at a position corresponding to codon 4046 (codon 4095 in transcript ENSMUST00000023352.8) created a premature stop codon (p.Y4046*).

Allele

Symbol: Alox15^tm1Fun
Name: targeted mutation 1, Colin D Funk
MGI accession ID: MGI:1857428
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Alox15
Marker name: arachidonate 15-lipoxygenase
Chromosome: 11
Strain of origin: 129S2/SvPas
Molecular note: Insertion of a neomycin resistance cassette into exon 3 disrupted the Alox15 gene. Northern blot analyses of resident peritoneal macrophages and of bone marrow-derived macrophages did not detect transcript in homozygous mutant mice. Western blot analysis of resident peritoneal macrophages did not detect ALOX15 in homozygous mutant mice.