In this strain, a neomycin resistance cassette replaces part of exon 6 of the endogenous Cyp7b1 gene. These mice are useful for studying cholesterol and bile acid metabolism, oxysterol synthesis and breakdown, macrophage function, and B cell trafficking and activation in the immune system.
Dr. David Russell, Univ of Texas Southwest Med Ctr Dallas
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Cyp7b1 | cytochrome P450, family 7, subfamily b, polypeptide 1 |
In this strain, a neomycin resistance (neo) cassette replaces part of exon 6 of the endogenous cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1) gene, abolishing gene function. Homozygotes are viable and fertile. Cyp7b1 encodes oxysterol 7α-hydroxylase, an enzyme of the alternate bile acid synthesis pathway involved in cholesterol homeostasis. Plasma and tissue levels of 25- and 27-hydroxycholesterol, two oxysterol bioactive lipid substrates of oxysterol 7α-hydroxylase, were elevated in homozygotes. Sterol biosynthetic rates showed a 40% decrease in male kidneys. These mice are useful for studying cholesterol and bile acid metabolism, oxysterol synthesis and breakdown, macrophage function, and B cell trafficking and activation in the immune system.
A targeting vector was designed to replace part of exon 6 encoding the cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129/SvEv-derived I1C embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were bred to C57BL/6J females. These mice were maintained on a mixed C57BL/6J x 129 background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, David W Russell |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Cyp7b1- |
Gene Symbol and Name | Cyp7b1, cytochrome P450, family 7, subfamily b, polypeptide 1 |
Gene Synonym(s) | |
Strain of Origin | 129S/SvEv |
Chromosome | 3 |
General Note | ES cell line = I1C. |
Molecular Note | The gene was disrupted by replacing the majority of exon 6 (encoding amino acids 410-507) with a neomycin resistance cassette. Homozygous mutant animals lacked enzyme activity as determined by assay of hepatic lysates. Northern blot anlaysis of homozygous animals showed expression of mutant RNA species of larger size compared to wild-type, presumably from abnormal splicing of the gene product. Western blot analysis using polyclonal antibodies recognizing amino acids 266-281 did not detect protein product in liver homogenates from homozygous mutant animals. |
Mutations Made By | Dr. David Russell, Univ of Texas Southwest Med Ctr Dallas |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6;129S-Cyp7b1tm1Rus/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #016108 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Cyp7b1<tm1Rus> |
Frozen Mouse Embryo | B6;129S-Cyp7b1<tm1Rus>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S-Cyp7b1<tm1Rus>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S-Cyp7b1<tm1Rus>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S-Cyp7b1<tm1Rus>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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