JAX Mouse Strain Datasheet - 015858

C.129S4(B6)-Arg1^tm1Lky/J

Stock No:: 015858

Cryo Recovery

Overview

A targeting vector was designed to insert an internal ribosome entry site (IRES)-enhanced yellow fluorescent protein (eYFP) fusion protein, downstream of the endogenous stop codon of the arginase (Arg1) gene. These mice may be useful for visualization of arginase-containing cells and their response to infection.

Donating Investigator

Richard M. Locksley, Univ of California San Francisco-HHMI

Genetic overview

Genetic Background	Generation

Arg1^tm1Lky

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter)	Arg1	arginase, liver

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Research Applications

Cell Biology Research
Metabolism Research
Research Tools

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

A targeting vector was designed to insert an internal ribosome entry site (IRES)-enhanced yellow fluorescent protein (eYFP) fusion protein, downstream of the endogenous stop codon of the arginase (Arg1) gene. These homozygous YARG mice are viable, fertile, and normal in size. Located in the cytoplasm of the liver and specifically in macrophages, arginase I converts L-arginine into L-ornithine and urea as the final step in the urea cycle. L-arginine can also be catabolized into Nitric oxide (NO), a secreted free radical which causes tissues damage and is also toxic to bacteria. Arginase I suppresses NO production by decreasing the amount of L-arginine available to the nitric oxide synthase (NOS) enzyme for conversion into NO and other reactive oxygen species (ROS). Nine days after infection with the helminth, Nippostrongylus brasiliensis, YARG mice exhibit accumulation of arginase I-expressing macrophages. As soon as two days after inoculation with chitin, a polysaccharide found in the exoskeleton of some invertebrates, these macrophages were present in the lung and peritoneum. These mice may be useful for visualization of arginase-containing cells and their response to infection.

Development

A targeting vector was designed to insert a floxed neomycin (neo) resistance cassette, followed by an internal ribosome entry site (IRES)-enhanced yellow fluorescent protein (eYFP) fusion protein, downstream of the endogenous stop codon of the arginase (Arg) gene. The construct was electroporated into 129S4/SvJae-derived PC3 embryonic stem (ES) cells. These PC3 cells contain a transgene expressing Cre recombinase under control of the protamine promoter (Tg(Prm-cre)700g), to delete the neo cassette in the male germline. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6 females. The male offspring from this initial breeding lack the floxed neo cassette and were bred to female C57BL/6 mice to establish a colony of YARG mice. These mutant mice were backcrossed to BALB/cJ mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to BALB/cJ (Stock No. 000651) for at least one generation.

Expression Data

Expressed Gene	YFP, Yellow Fluorescent Protein, jellyfish
Site of Expression	YARG mice exhibit accumulation of arginase I-expressing macrophages in the lung and peritoneum after helminth infection.

Control Suggestions

Wild-type from the colony

Approximate Controls

000651 BALB/cJ

Additional Information

Considerations for Choosing Controls

Selected References

Reese TA; Liang HE; Tager AM; Luster AD; Van Rooijen N; Voehringer D; Locksley RM. 2007. Chitin induces accumulation in tissue of innate immune cells associated with allergy. Nature 447(7140):92-6. PubMed: 17450126 MGI: J:122735

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Genetics

Arg1^tm1Lky

Allele Symbol: Arg1^tm1Lky

Allele Name	targeted mutation 1, Richard M Locksley
Allele Type	Targeted (Reporter)
Allele Synonym(s)	Arg1^YFP; Arg1^Yarg; YARG
Gene Symbol and Name	Arg1, arginase, liver
Gene Synonym(s)	AI; AI256583; Arg-1; Arg-1; PGIF; expressed sequence AI256583
Expressed Gene	YFP, Yellow Fluorescent Protein, jellyfish
Site of Expression	YARG mice exhibit accumulation of arginase I-expressing macrophages in the lung and peritoneum after helminth infection.
Strain of Origin	129S4/SvJae-Tg(Prm-cre)70Og
Chromosome	10
Molecular Note	An IRES element linked to an enhanced YFP construct was inserted in exon 8 downstream of the endogenous stop codon and a floxed neomycin cassette. The neomycin selection cassette was excised in male mice by cre expressed from the Prm1 promoter.
Mutations Made By	Richard Locksley, Univ of California San Francisco-HHMI

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Cell Biology Research
- Signal Transduction
Metabolism Research
- Free Radical Research
Research Tools
- Genetics Research
  - Tissue/Cell Markers
- Immunology, Inflammation and Autoimmunity Research
- Toxicology Research
  - free radical research

Genotype: YFP related

Research Tools
- Fluorescent Proteins

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Arg1^tm1Lky/Arg1^tm1Lky
involves: 129S4/SvJae * C57BL/6

normal phenotype

no abnormal phenotype detected

References

Reese TA; Liang HE; Tager AM; Luster AD; Van Rooijen N; Voehringer D; Locksley RM. 2007. Chitin induces accumulation in tissue of innate immune cells associated with allergy. Nature 447(7140):92-6. PubMed: 17450126 MGI: J:122735

Additional - Arg1^tm1Lky related

Abdolazimi Y; Stojanova Z; Segil N. 2016. Selection of cell fate in the organ of Corti involves the integration of Hes/Hey signaling at the Atoh1 promoter. Development 143(5):841-50. PubMed: 26932672 MGI: J:229889
Gabanyi I; Muller PA; Feighery L; Oliveira TY; Costa-Pinto FA; Mucida D. 2016. Neuro-immune Interactions Drive Tissue Programming in Intestinal Macrophages. Cell 164(3):378-91. PubMed: 26777404 MGI: J:230661
Heredia JE; Mukundan L; Chen FM; Mueller AA; Deo RC; Locksley RM; Rando TA; Chawla A. 2013. Type 2 innate signals stimulate fibro/adipogenic progenitors to facilitate muscle regeneration. Cell 153(2):376-88. PubMed: 23582327 MGI: J:214506
Molofsky AB; Nussbaum JC; Liang HE; Van Dyken SJ; Cheng LE; Mohapatra A; Chawla A; Locksley RM. 2013. Innate lymphoid type 2 cells sustain visceral adipose tissue eosinophils and alternatively activated macrophages. J Exp Med 210(3):535-49. PubMed: 23420878 MGI: J:219842
Patnode ML; Bando JK; Krummel MF; Locksley RM; Rosen SD. 2014. Leukotriene B4 amplifies eosinophil accumulation in response to nematodes. J Exp Med 211(7):1281-8. PubMed: 24889202 MGI: J:214462
Van Dyken SJ; Mohapatra A; Nussbaum JC; Molofsky AB; Thornton EE; Ziegler SF; McKenzie AN; Krummel MF; Liang HE; Locksley RM. 2014. Chitin activates parallel immune modules that direct distinct inflammatory responses via innate lymphoid type 2 and gammadelta T cells. Immunity 40(3):414-24. PubMed: 24631157 MGI: J:210323
Wu D; Molofsky AB; Liang HE; Ricardo-Gonzalez RR; Jouihan HA; Bando JK; Chawla A; Locksley RM. 2011. Eosinophils sustain adipose alternatively activated macrophages associated with glucose homeostasis. Science 332(6026):243-7. PubMed: 21436399 MGI: J:170307

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Arg1<tm1Lky>

Progeny testing is not required

The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks.

The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

Terms Of Use

Terms of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

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Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

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The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Arg1tm1Lky

Allele Symbol: Arg1tm1Lky

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: YFP related

Mammalian Phenotype Terms by Genotype

Genotype: Arg1tm1Lky/Arg1tm1Lkyinvolves: 129S4/SvJae * C57BL/6

normal phenotype

References

Additional - Arg1tm1Lky related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Progeny testing is not required

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Arg1^tm1Lky

Allele Symbol: Arg1^tm1Lky

Genotype: Arg1^tm1Lky/Arg1^tm1Lky
involves: 129S4/SvJae * C57BL/6

Additional - Arg1^tm1Lky related