A targeting vector was designed to insert an internal ribosome entry site (IRES)-enhanced yellow fluorescent protein (eYFP) fusion protein, downstream of the endogenous stop codon of the arginase (Arg1) gene. These mice may be useful for visualization of arginase-containing cells and their response to infection.
Richard M. Locksley, Univ of California San Francisco-HHMI
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter) | Arg1 | arginase, liver |
A targeting vector was designed to insert an internal ribosome entry site (IRES)-enhanced yellow fluorescent protein (eYFP) fusion protein, downstream of the endogenous stop codon of the arginase (Arg1) gene. These homozygous YARG mice are viable, fertile, and normal in size. Located in the cytoplasm of the liver and specifically in macrophages, arginase I converts L-arginine into L-ornithine and urea as the final step in the urea cycle. L-arginine can also be catabolized into Nitric oxide (NO), a secreted free radical which causes tissues damage and is also toxic to bacteria. Arginase I suppresses NO production by decreasing the amount of L-arginine available to the nitric oxide synthase (NOS) enzyme for conversion into NO and other reactive oxygen species (ROS). Nine days after infection with the helminth, Nippostrongylus brasiliensis, YARG mice exhibit accumulation of arginase I-expressing macrophages. As soon as two days after inoculation with chitin, a polysaccharide found in the exoskeleton of some invertebrates, these macrophages were present in the lung and peritoneum. These mice may be useful for visualization of arginase-containing cells and their response to infection.
A targeting vector was designed to insert a floxed neomycin (neo) resistance cassette, followed by an internal ribosome entry site (IRES)-enhanced yellow fluorescent protein (eYFP) fusion protein, downstream of the endogenous stop codon of the arginase (Arg) gene. The construct was electroporated into 129S4/SvJae-derived PC3 embryonic stem (ES) cells. These PC3 cells contain a transgene expressing Cre recombinase under control of the protamine promoter (Tg(Prm-cre)700g), to delete the neo cassette in the male germline. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6 females. The male offspring from this initial breeding lack the floxed neo cassette and were bred to female C57BL/6 mice to establish a colony of YARG mice. These mutant mice were backcrossed to BALB/cJ mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to BALB/cJ (Stock No. 000651) for at least one generation.
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
---|---|
Site of Expression | YARG mice exhibit accumulation of arginase I-expressing macrophages in the lung and peritoneum after helminth infection. |
Allele Name | targeted mutation 1, Richard M Locksley |
---|---|
Allele Type | Targeted (Reporter) |
Allele Synonym(s) | Arg1Yarg; Arg1YFP; YARG |
Gene Symbol and Name | Arg1, arginase, liver |
Gene Synonym(s) | |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Site of Expression | YARG mice exhibit accumulation of arginase I-expressing macrophages in the lung and peritoneum after helminth infection. |
Strain of Origin | 129S4/SvJae-Tg(Prm-cre)70Og |
Chromosome | 10 |
Molecular Note | An IRES element linked to an enhanced YFP construct was inserted in exon 8 downstream of the endogenous stop codon and a floxed neomycin cassette. The neomycin selection cassette was excised in male mice by cre expressed from the Prm1 promoter. |
Mutations Made By | Richard Locksley, Univ of California San Francisco-HHMI |
When maintaining a live colony, homozygous mice may be bred together.
When using the C.129S4(B6)-Arg1tm1Lky/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #015858 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Arg1<tm1Lky> |
Frozen Mouse Embryo | C.129S4(B6)-Arg1<tm1Lky>/J | $2595.00 |
Frozen Mouse Embryo | C.129S4(B6)-Arg1<tm1Lky>/J | $2595.00 |
Frozen Mouse Embryo | C.129S4(B6)-Arg1<tm1Lky>/J | $3373.50 |
Frozen Mouse Embryo | C.129S4(B6)-Arg1<tm1Lky>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.