B6;DBA-Tg(Cited1-TagRFP)26Amc/J

Stock number: 015853
Research areas: Developmental Biology Research, Reproductive Biology Research, Research Tools

Description

This Cited1-nuc-TagRFP-T transgenic strain expresses Red Fluorescent Protein in the developing kidney cap mesenchyme and male reproductive systems and may be useful in studies of genitourinary development.

Detailed description

These transgenic mice express the Tag-RFPT variant of Red Fluorescent Protein under the direction of the mouse Cited1, Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 1, promoter. TagRFP is the optimized monomeric derivative of the tetrameric eqFP578 from the sea anemone, Entacmaea quadricolor. RFP fluorescence is detected in the cap mesenchyme in the kidney and the male reproductive systems of hemizygous embryos, 15.5 embryonic days of age. Immunohistochemical analysis reveals that the TagRFP-T transgene is expressed in the expected Cited1 expression domain. Other possible sites of expression have not been characterized. Mice that are hemizygous for the knock in allele are viable, fertile, normal in size and do not display physical or behavioral abnormalities.



This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP).

Development

A targeting construct containing the Tag-RFPT variant of Red Fluorescent Protein and FRT site flanked Kanamycin/neomycin selection cassette was inserted into the consensus start ATG codon of the gene in a BAC (RP23-204G22). The PGK-Neo/Kan cassette was removed by transient Flpe-recombinase expression. The resulting BAC transgene was microinjected into (C57BL/6 x DBA) F1 donor eggs. Founder line 26 was subsequently established. The mice were then crossed to C57BL/6 for two generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Tg(Cited1-TagRFP)26Amc
Name: transgene insertion 26, Andrew P McMahon
MGI accession ID: MGI:4940389
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Cited1-TagRFP)26Amc
Marker name: transgene insertion 26, Andrew P McMahon
Chromosome: UN
Strain of origin: (C57BL/6 x DBA)F1
Expressed genes: RFP,Red Fluorescent Protein,coral
Site of expression: RFP fluorescence is detected in the cap mesenchyme in the kidney and the male reproductive system.
Molecular note: These transgenic mice express the Tag-RFPT variant of Red Fluorescent Protein under the direction of the mouse Cited1, Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 1, promoter. TagRFP is the optimized monomeric derivative of the tetrameric eqFP578 from the sea anemone, Entacmaea quadricolor. RFP fluorescence is detected in the cap mesenchyme in the kidney and the male reproductive systems of hemizygous embryos, 15.5 embryonic days of age. Immunohistochemical analysis reveals that the TagRFP-T transgene is expressed in the expected Cited1 expression domain. Other possible sites of expression have not been characterized.