This Cited1-nuc-TagRFP-T transgenic strain expresses Red Fluorescent Protein in the developing kidney cap mesenchyme and male reproductive systems and may be useful in studies of genitourinary development.
Andrew P McMahon, University of Southern California
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
These transgenic mice express the Tag-RFPT variant of Red Fluorescent Protein under the direction of the mouse Cited1, Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 1, promoter. TagRFP is the optimized monomeric derivative of the tetrameric eqFP578 from the sea anemone, Entacmaea quadricolor.
RFP fluorescence is detected in the cap mesenchyme in the kidney and the male reproductive systems of hemizygous embryos, 15.5 embryonic days of age. Immunohistochemical analysis reveals that the TagRFP-T transgene is expressed in the expected Cited1 expression domain. Other possible sites of expression have not been characterized. Mice that are hemizygous for the knock in allele are viable, fertile, normal in size and do not display physical or behavioral abnormalities.
This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP).
A targeting construct containing the Tag-RFPT variant of Red Fluorescent Protein and FRT site flanked Kanamycin/neomycin selection cassette was inserted into the consensus start ATG codon of the gene in a BAC (RP23-204G22). The PGK-Neo/Kan cassette was removed by transient Flpe-recombinase expression. The resulting BAC transgene was microinjected into (C57BL/6 x DBA) F1 donor eggs. Founder line 26 was subsequently established. The mice were then crossed to C57BL/6 for two generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | RFP, Red Fluorescent Protein, coral |
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Site of Expression | RFP fluorescence is detected in the cap mesenchyme in the kidney and the male reproductive system. |
Allele Name | transgene insertion 26, Andrew P McMahon |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Cited1 nuc-TagRFP-T |
Gene Symbol and Name | Tg(Cited1-TagRFP)26Amc, transgene insertion 26, Andrew P McMahon |
Gene Synonym(s) | |
Promoter | Cited1, Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 1, mouse, laboratory |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Site of Expression | RFP fluorescence is detected in the cap mesenchyme in the kidney and the male reproductive system. |
Strain of Origin | (C57BL/6 x DBA)F1 |
Chromosome | UN |
Molecular Note | These transgenic mice express the Tag-RFPT variant of Red Fluorescent Protein under the direction of the mouse Cited1, Cbp/p300-interacting transactivator with Glu/Asp-rich carboxy-terminal domain 1, promoter. TagRFP is the optimized monomeric derivative of the tetrameric eqFP578 from the sea anemone, Entacmaea quadricolor. RFP fluorescence is detected in the cap mesenchyme in the kidney and the male reproductive systems of hemizygous embryos, 15.5 embryonic days of age. Immunohistochemical analysis reveals that the TagRFP-T transgene is expressed in the expected Cited1 expression domain. Other possible sites of expression have not been characterized. |
Mutations Made By | Dr. Pumin Zhang, Baylor College of Medicine |
When maintaining a live colony, these mice can be bred as hemizygotes.
When using the B6;DBA-Tg(Cited1-TagRFP)26Amc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #015853 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Cited1-TagRFP)26Amc |
Frozen Mouse Embryo | B6;DBA-Tg(Cited1-TagRFP)26Amc/J | $2595.00 |
Frozen Mouse Embryo | B6;DBA-Tg(Cited1-TagRFP)26Amc/J | $2595.00 |
Frozen Mouse Embryo | B6;DBA-Tg(Cited1-TagRFP)26Amc/J | $3373.50 |
Frozen Mouse Embryo | B6;DBA-Tg(Cited1-TagRFP)26Amc/J | $3373.50 |
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