This transgene expresses codon-improved Cre recombinase driven by the rhodopsin promoter for localization to the retinal rod cells, primarily in the outer nuclear layer. It is valuable for studies involving localized expression modification in retinal rod cells.
Read More +Genetic Background | Generation |
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N10F2
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Allele Type | Gene Symbol | Gene Name |
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Not Applicable | Pde6b | phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide |
Allele Type |
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Transgenic (Recombinase-expressing) |
Although Woodford et al. reported that this 4 kb promoter is active in both rods and cones, expression of codon-improved Cre recombinase from the Tg(Rho-icre)1Ck transgene has been shown to be absent from cones and restricted to rods, where it is expressed in the retinal outer nuclear layer. Cre-mediated excision is not detected at 4 days of age, but is detected at 7 days of age, and is maximally active by postnatal day 18 when the level of excision detected equals that detected in adults. In the initial assessment, no abnormality was detected in the retinal morphology or in scotopic or photopic electroretinograms of hemizygotes even at 8 months of age (Li et al., 2005). Because the independently acquired Tg(Rho-icre)1Ck strain used by Sundermeier et al. (PMID: 16908407) was found to also have a contaminating transgene, with bovine RGS9Bp driven by the opsin promoter, genotyping was done to confirm that this strain lacks that contaminating transgene.
The construct for Tg(Rho-icre)1Ck was injected into fertilized F1 hybrid oocytes from a cross of SJL and C57BL/6JTac and the resultant transgenic was bred to C57BL/6Tac to remove the SJL-derived Pde6brd1 from this line. The line was maintained on a mixed B6Tac;SJL background homozygous for Pde6b+ until it was imported into The Jackson Laboratory by hysterectomy rederivation by breeding once to C57BL/6J and then this line was backcrossed once more to C57BL/6J before maintaining by sibling intercrossing for 5 generations and then resuming backcrossing to C57BL/6J in 2015, and subsequently sibling intercrossing again in an effort to make this strain homozygous.
Expressed Gene | Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide, mouse, laboratory |
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Site of Expression | Retina. |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression |
Allele Name | wild type |
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Allele Type | Not Applicable |
Allele Synonym(s) | |
Gene Symbol and Name | Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide |
Gene Synonym(s) | |
Expressed Gene | Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide, mouse, laboratory |
Site of Expression | Retina. |
Strain of Origin | Not Applicable |
Chromosome | 5 |
Allele Name | transgene insertion 1, Ching-Kang Chen |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | iCre75; iCre-75; RCre; Tg(Opsin-iCre); Tg(Rho-cre)1Ck |
Gene Symbol and Name | Tg(Rho-icre)1Ck, transgene insertion 1, Ching-Kang Chen |
Gene Synonym(s) | |
Promoter | Rho, rhodopsin, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Strain of Origin | C57BL/6 x SJL |
Chromosome | UN |
Molecular Note | A 4-kb rhodopsin promoter was used to drive expression of bacteriophage P1 Cre recombinase in the outer nuclear layer of mutant retinas. Cre expression was found in rods and not in cones. |
When using the B6.Cg-Pde6b+ Tg(Rho-icre)1Ck/Boc mouse strain in a publication, please cite the originating article(s) and include JAX stock #015850 in your Materials and Methods section.
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