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B6.Cg-Pde6b+ Tg(Rho-icre)1Ck/Boc
Stock No: 015850
  • Transgenic
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  • Overview
  • Details
    • Detailed Description
    • Development
    • Expression Data
    • Control Suggestions
    • Selected References
  • Genetics
  • Disease/Phenotype
    • Disease Terms
    • Research Areas By Phenotype
    • Mammalian Phenotype Terms by Genotype
    • References
  • Technical Support
    • Genotyping Protocols
    • Mating System
    • Breeder Pairs
    • Citation
    • Animal Health Reports
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  • Terms Of Use
    • Additional Use Restrictions
  • Related Strains

Overview

This transgene expresses codon-improved Cre recombinase driven by the rhodopsin promoter for localization to the retinal rod cells, primarily in the outer nuclear layer. It is valuable for studies involving localized expression modification in retinal rod cells.

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Genetic overview

Genetic Background Generation
N10F2
(2020-01-24 00:00:00)

Pde6b+

Allele Type Gene Symbol Gene Name
Not Applicable Pde6b phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide

Tg(Rho-icre)1Ck

Allele Type
Transgenic (Recombinase-expressing)
View Genetics

Research Applications

  • Mouse/Human Gene Homologs
  • Sensorineural Research
  • Research Tools
View All Research Applications

Base Price

Starting at:

$257.36 Domestic price for female
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Details

Detailed Description

Although Woodford et al. reported that this 4 kb promoter is active in both rods and cones, expression of codon-improved Cre recombinase from the Tg(Rho-icre)1Ck transgene has been shown to be absent from cones and restricted to rods, where it is expressed in the retinal outer nuclear layer. Cre-mediated excision is not detected at 4 days of age, but is detected at 7 days of age, and is maximally active by postnatal day 18 when the level of excision detected equals that detected in adults. In the initial assessment, no abnormality was detected in the retinal morphology or in scotopic or photopic electroretinograms of hemizygotes even at 8 months of age (Li et al., 2005). Because the independently acquired Tg(Rho-icre)1Ck strain used by Sundermeier et al. (PMID: 16908407) was found to also have a contaminating transgene, with bovine RGS9Bp driven by the opsin promoter, genotyping was done to confirm that this strain lacks that contaminating transgene.

Development

The construct for Tg(Rho-icre)1Ck was injected into fertilized F1 hybrid oocytes from a cross of SJL and C57BL/6JTac and the resultant transgenic was bred to C57BL/6Tac to remove the SJL-derived Pde6brd1 from this line. The line was maintained on a mixed B6Tac;SJL background homozygous for Pde6b+ until it was imported into The Jackson Laboratory by hysterectomy rederivation by breeding once to C57BL/6J and then this line was backcrossed once more to C57BL/6J before maintaining by sibling intercrossing for 5 generations and then resuming backcrossing to C57BL/6J in 2015, and subsequently sibling intercrossing again in an effort to make this strain homozygous.

Expression Data

Expressed Gene Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide, mouse, laboratory
Site of ExpressionRetina.
Expressed Gene cre, cre recombinase, bacteriophage P1
Site of Expression

Control Suggestions

  • Noncarrier

Additional Information

  • Considerations for Choosing Controls

Selected References

  • Li S; Chen D; Sauve Y; McCandless J; Chen YJ; Chen CK. 2005. Rhodopsin-iCre transgenic mouse line for Cre-mediated rod-specific gene targeting. Genesis 41(2):73-80PubMed: 15682388MGI: J:97322
View All References

Genetics

Pde6b+

Allele Symbol: Pde6b+

Allele Name wild type
Allele Type Not Applicable
Allele Synonym(s)
Gene Symbol and Name Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide
Gene Synonym(s)
Expressed GenePde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide, mouse, laboratory
Site of ExpressionRetina.
Strain of Origin Not Applicable
Chromosome 5

Tg(Rho-icre)1Ck

Allele Symbol: Tg(Rho-icre)1Ck

Allele Name transgene insertion 1, Ching-Kang Chen
Allele Type Transgenic (Recombinase-expressing)
Allele Synonym(s) iCre75; iCre-75; RCre; Tg(Opsin-iCre); Tg(Rho-cre)1Ck
Gene Symbol and Name Tg(Rho-icre)1Ck, transgene insertion 1, Ching-Kang Chen
Gene Synonym(s)
PromoterRho, rhodopsin, mouse, laboratory
Expressed Genecre, cre recombinase, bacteriophage P1
Strain of Origin C57BL/6 x SJL
Chromosome UN
Molecular Note A 4-kb rhodopsin promoter was used to drive expression of bacteriophage P1 Cre recombinase in the outer nuclear layer of mutant retinas. Cre expression was found in rods and not in cones.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Genotype: Pde6b+ related

  • Mouse/Human Gene Homologs
    • retinitis pigmentosa, wildtype
  • Sensorineural Research
    • Retinal Degeneration
      • wild-type

Genotype: Tg(Rho-icre)1Ck related

  • Sensorineural Research
  • Research Tools
    • Sensorineural Research

Mammalian Phenotype Terms by Genotype

References

  • Li S; Chen D; Sauve Y; McCandless J; Chen YJ; Chen CK. 2005. Rhodopsin-iCre transgenic mouse line for Cre-mediated rod-specific gene targeting. Genesis 41(2):73-80PubMed: 15682388MGI: J:97322

Additional - Pde6b+ related

Additional - Tg(Rho-icre)1Ck related

Technical Support

CONTACT TECHNICAL SUPPORT
  • Genotyping Protocols

    • Genotyping resources and troubleshooting
  • Mating System

    • Tg/? x Tg/?
  • Citation

    When using the B6.Cg-Pde6b+ Tg(Rho-icre)1Ck/Boc mouse strain in a publication, please cite the originating article(s) and include JAX stock #015850 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

G3A (Intermediate)

Pricing & Availability

Availability Varies
  • Domestic
  • International

Live Mouse

Age Genotype Price
weeks

Cryorecovery - Pricing

Service/Product Description Price
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Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470
Email: TechTran@jax.org

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