STOCK Syt12^tm1.1Sud/J

Stock number: 015836
Product name: Syt12 S97A KI
Research areas: Neurobiology Research, Research Tools

Description

This Syt12 (synaptotagmin XII) mutant strain combines an S97A amino acid mutation with loxP sites that enable cre-mediated elimination of tissue-specific expression. This strain may be useful in studies of neurotransmission and the presynaptic function of Syt12 phosphorylation.

Detailed description

Exon 4 of this Syt12 (synaptotagmin XII) targeted mutation strain is flanked by loxP sites and carries a serine to alanine mutation at residue 97 (S97A). Serine 97 is a protein kinase A (PKA) phosphorylation site and the point mutation abolishes the increase of spontaneous neurotransmitter release by Syt12 overexpression in primary cortical culture. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. Floxed mice express the targeted gene at levels comparable to wild type, as demonstrated by Western blot of whole brain.

Development

Serine 97 (exon 4) was mutated to alanine (S97A) and exons 4-6 were flanked by loxP sites in the targeting vector. An FRT-flanked neomycin cassette was also incorporated. The mutation was created in (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. The neomycin selection cassette was excised from chimeric animals though crosses with an Actb beta actin promoter Flp recombinase mouse originally made on a B6SJLF2 background and backcrossed to C57BL/6NCrl. This strain was maintained on a predominantly C57BL/6 and 129 mixed background by the donating laboratory.

Allele

Symbol: Syt12^tm1.1Sud
Name: targeted mutation 1.1, Thomas C Sudhof
MGI accession ID: MGI:4940391
Generation method: Targeted
Marker symbol: Syt12
Marker name: synaptotagmin XII
Chromosome: 19
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Serine 97 (exon 4) was mutated to alanine (S97A) and exons 4-6 were flanked by loxP sites in the targeting vector. An FRT-flanked neomycin cassette was also incorporated. Flp-mediated recombination removed the neo cassette.