This Syt12 (synaptotagmin XII) mutant strain combines an S97A amino acid mutation with loxP sites that enable cre-mediated elimination of tissue-specific expression. This strain may be useful in studies of neurotransmission and the presynaptic function of Syt12 phosphorylation.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted | Syt12 | synaptotagmin XII |
Exon 4 of this Syt12 (synaptotagmin XII) targeted mutation strain is flanked by loxP sites and carries a serine to alanine mutation at residue 97 (S97A). Serine 97 is a protein kinase A (PKA) phosphorylation site and the point mutation abolishes the increase of spontaneous neurotransmitter release by Syt12 overexpression in primary cortical culture. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. Floxed mice express the targeted gene at levels comparable to wild type, as demonstrated by Western blot of whole brain.
Serine 97 (exon 4) was mutated to alanine (S97A) and exons 4-6 were flanked by loxP sites in the targeting vector. An FRT-flanked neomycin cassette was also incorporated. The mutation was created in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. The neomycin selection cassette was excised from chimeric animals though crosses with an Actb beta actin promoter Flp recombinase mouse originally made on a B6SJLF2 background and backcrossed to C57BL/6NCrl. This strain was maintained on a predominantly C57BL/6 and 129 mixed background by the donating laboratory.
Allele Name | targeted mutation 1.1, Thomas C Sudhof |
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Allele Type | Targeted |
Allele Synonym(s) | Syt12 S97A KI |
Gene Symbol and Name | Syt12, synaptotagmin XII |
Gene Synonym(s) | |
Promoter | Syt12, synaptotagmin XII, mouse, laboratory |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 19 |
Molecular Note | Serine 97 (exon 4) was mutated to alanine (S97A) and exons 4-6 were flanked by loxP sites in the targeting vector. An FRT-flanked neomycin cassette was also incorporated. Flp-mediated recombination removed the neo cassette. |
Mutations Made By | Dr. Thomas Sudhof, Stanford University School of Medicine |
When maintained as a live colony, heterozygotes may be bred.
When using the Syt12 S97A KI mouse strain in a publication, please cite the originating article(s) and include JAX stock #015836 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Syt12<tm1.1Sud> |
Frozen Mouse Embryo | STOCK Syt12<tm1.1Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Syt12<tm1.1Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Syt12<tm1.1Sud>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Syt12<tm1.1Sud>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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