These neuroligin 3 (Nlgn3) targeted mutation mice carry an R704C mutation that reduces expression levels and impairs excitatory glutamatergic synaptic transmission.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Arginine 704 of the Nlgn3 (neuroligin 3) gene was mutated to cysteine (R704C) in this targeted mutation strain. Expression levels of this X-linked allele are reduced to approximately 70% of wild type levels in brain tissue. This mutation causes a selective impairment of excitatory glutamatergic synaptic transmission mediated by AMPA receptors in both cultured hippocampal neurons and acute hippocampal slices.
Arginine 704 (exon 8) was mutated to encode cysteine (R704C) and an FRT-flanked neomycin cassette (with a single, un-used loxP site) was placed in intron 7. The mutation was introduced to (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. The neomycin selection cassette was excised from chimeric animals though crosses with an Actb beta actin promoter Flp recombinase mouse originally made on a B6SJLF2 background, and backcrossed to C57BL/6NCrl. This strain was maintained on a mixed 129 and C57BL/6 genetic background by the donating laboratory through alternated crosses of heterozygous females with wildtype 129S2/SvPasCrl and C57BL/6NCrl males to enhance breeding performance.
|Allele Name||targeted mutation 3.1, Thomas C Sudhof|
|Allele Type||Targeted (Not Specified)|
|Allele Synonym(s)||Neuroligin 3 R704C; NL3R704C|
|Gene Symbol and Name||Nlgn3, neuroligin 3|
|Promoter||Nlgn3, neuroligin 3, mouse, laboratory|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||Arginine 704 (exon 8) was mutated to encode cysteine (R704C) and an FRT-flanked neomycin cassette (with a single, un-used loxP site) was placed in intron 7. Flp-mediated recombination removed the neo cassette.|
|Mutations Made By|| |
Dr. Thomas Sudhof, Stanford University School of Medicine
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the Neuroligin 3 R704C mouse strain in a publication, please cite the originating article(s) and include JAX stock #015834 in your Materials and Methods section.