STOCK Rims2^tm1.1Sud/J

Stock number: 015833
Product name: R2F-RIM2 alpha/beta/gamma floxed; RIM2 f/f
Research areas: Neurobiology Research, Research Tools

Description

This floxed Rims2 (regulating synaptic membrane exocytosis 2) mutation strain enables cre-mediated excision of the α, β, and γ isoforms of the gene and may be useful in studies of synaptic neurotransmission.

Detailed description

These mice possess loxP sites on either side of exon 26 in the Rims2 (regulating synaptic membrane exocytosis 2) gene. An in-frame ECFP-tetracysteine tag is fused to the floxed exon, enabling immunofluorescent detection. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the α, β, and γ isoforms of the gene.

Development

Exon 26 (the first exon shared by the α, β, and γ isoforms of the gene) was fused to an in-frame ECFP-tetracysteine tag and flanked by loxP sites. A FRT-flanked neomycin selection cassette was placed immediately 5' of the floxed exon. The mutation was created in (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. The neomycin selection cassette was excised from chimeric animals though crosses with an Actb beta actin promoter Flp recombinase mouse originally made on a B6SJLF2 background, and possibly backcrossed to C57BL/6NCrl. This strain was maintained on a predominantly C57BL/6 and 129 mixed background by the donating laboratory.

Allele

Symbol: Rims2^tm1.1Sud
Name: targeted mutation 1.1, Thomas C Sudhof
MGI accession ID: MGI:4939895
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Rims2
Marker name: regulating synaptic membrane exocytosis 2
Chromosome: 15
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Exon 26 was replaced with an FRT-flanked neo cassette and a floxed ECFP-tetracysteine tagged exon 26. Flp-mediated recombination removed the neo cassette leaving the tagged exon 26 floxed.