This floxed Rims2 (regulating synaptic membrane exocytosis 2) mutation strain enables cre-mediated excision of the α, β, and γ isoforms of the gene and may be useful in studies of synaptic neurotransmission.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
These mice possess loxP sites on either side of exon 26 in the Rims2 (regulating synaptic membrane exocytosis 2) gene. An in-frame ECFP-tetracysteine tag is fused to the floxed exon, enabling immunofluorescent detection. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the α, β, and γ isoforms of the gene.
Exon 26 (the first exon shared by the α, β, and γ isoforms of the gene) was fused to an in-frame ECFP-tetracysteine tag and flanked by loxP sites. A FRT-flanked neomycin selection cassette was placed immediately 5' of the floxed exon. The mutation was created in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. The neomycin selection cassette was excised from chimeric animals though crosses with an Actb beta actin promoter Flp recombinase mouse originally made on a B6SJLF2 background, and possibly backcrossed to C57BL/6NCrl. This strain was maintained on a predominantly C57BL/6 and 129 mixed background by the donating laboratory.
|Allele Name||targeted mutation 1.1, Thomas C Sudhof|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Rims2, regulating synaptic membrane exocytosis 2|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||Exon 26 was replaced with an FRT-flanked neo cassette and a floxed ECFP-tetracysteine tagged exon 26. Flp-mediated recombination removed the neo cassette leaving the tagged exon 26 floxed.|
|Mutations Made By|| |
Dr. Thomas Sudhof, Stanford University School of Medicine
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the R2F-RIM2 alpha/beta/gamma floxed; RIM2 f/f mouse strain in a publication, please cite the originating article(s) and include JAX stock #015833 in your Materials and Methods section.