In this strain, a neomycin resistance (neo) cassette replaces exon 3 of the p21 protein (Cdc42/Rac)-activated kinase 5 (Pak5, formerly Pak7) gene, abolishing gene expression. Homozygotes are viable, fertile, normal in size, and do not display any gross physical abnormalities. Pak5 is a member of the group B family of PAK serine/threonine kinases and is expressed in the cortex, striatum, hypothalamus, cerebellum, and hippocampus. It is involved in neuronal growth and filopodia formation. The absence of a significant aberrant phenotype in these mice may be due to the compensatory actions of Pak6 (p21 protein (Cdc42/Rac)-activated kinase 6). When maintained on a background also lacking Pak6 activity, these mice have impaired cognitive function and deficits in learning and memory retention. These mice may be useful for studying learning, memory, and locomotion.
A targeting vector was designed to replace exon 3 encoding the p21 protein (Cdc42/Rac)-activated kinase 5 (Pak5) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6 females. These mice were backcrossed at least nine generations to C57BL/6 background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1 Audrey Minden|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Pak5, p21 (RAC1) activated kinase 5|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Exon 3 was replaced with a neomycin selection cassette inserted by homologous recombination. Protein was undetected by Western blot analysis of homozygous mutant brain extracts.|
|Mutations Made By|| |
Audrey Minden, Rutgers University
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129P2-Pak5tm1Amin/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #015827 in your Materials and Methods section.