These Pak5 (formerly Pak7) knock-out mice may be useful for studying learning, memory, and locomotion.
Audrey Minden, Rutgers University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Pak5 | p21 (RAC1) activated kinase 5 |
In this strain, a neomycin resistance (neo) cassette replaces exon 3 of the p21 protein (Cdc42/Rac)-activated kinase 5 (Pak5, formerly Pak7) gene, abolishing gene expression. Homozygotes are viable, fertile, normal in size, and do not display any gross physical abnormalities. Pak5 is a member of the group B family of PAK serine/threonine kinases and is expressed in the cortex, striatum, hypothalamus, cerebellum, and hippocampus. It is involved in neuronal growth and filopodia formation. The absence of a significant aberrant phenotype in these mice may be due to the compensatory actions of Pak6 (p21 protein (Cdc42/Rac)-activated kinase 6). When maintained on a background also lacking Pak6 activity, these mice have impaired cognitive function and deficits in learning and memory retention. These mice may be useful for studying learning, memory, and locomotion.
A targeting vector was designed to replace exon 3 encoding the p21 protein (Cdc42/Rac)-activated kinase 5 (Pak5) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6 females. These mice were backcrossed at least nine generations to C57BL/6 background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1 Audrey Minden |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | PAK5- |
Gene Symbol and Name | Pak5, p21 (RAC1) activated kinase 5 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 2 |
Molecular Note | Exon 3 was replaced with a neomycin selection cassette inserted by homologous recombination. Protein was undetected by Western blot analysis of homozygous mutant brain extracts. |
Mutations Made By | Audrey Minden, Rutgers University |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129P2-Pak5tm1Amin/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #015827 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Pak7<tm1Amin> |
Frozen Mouse Embryo | B6.129P2-Pak7<tm1Amin>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Pak7<tm1Amin>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Pak7<tm1Amin>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129P2-Pak7<tm1Amin>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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