These mice contain loxP sites flanking exon 2 of the targeted PRKC, apoptosis, WT1, regulator (Pawr or Par-4) gene. These mutant mice may be useful in generating conditional mutations for studying tumor development and treatment.
Vivek M Rangnekar, University of Kentucky
Mice homozygous for this Par4flox allele are viable and fertile, with loxP sites flanking exon 2 of the targeted PRKC, apoptosis, WT1, regulator (Pawr or Par-4) gene. Homozygotes are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. PAR4 is a pro-apoptopic protein capable of inducing apoptosis in cancer cells and causing regression of tumors in animal models. PAR4 interacts with, and inhibit, atypical protein kinase C isoforms, functioning as a negative regulator of the NF-κB pathway. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exon 2 deleted in the cre-expressing tissue, resulting in inactivation of Pawr gene function. Loss of PAR4 leads to a reduction in apoptosis by increased activation of NF-κB. These mutant mice may be useful in generating conditional mutations for studying tumor development and treatment.
A targeting vector was designed to insert loxP sites flanking exon 2 of the PRKC, apoptosis, WT1, regulator (Pawr or Par-4) gene. The construct was electroporated into C57BL/6NTac-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6NTac females. Upon arrival at The Jackson Laboratory Repository, mice were bred to C57BL/6NJ (Stock No. 005304) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Vivek Rangnekar|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Pawr, PRKC, apoptosis, WT1, regulator|
|Strain of Origin||C57BL/6NTac|
|Molecular Note||A targeting vector was designed to insert loxP sites flanking exon 2.|
|Mutations Made By|| |
Vivek Rangnekar, University of Kentucky
When maintaining a live colony, homozygous mice may be bred together.
When using the C57BL/6N-Pawrtm1Rang/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #015823 in your Materials and Methods section.