This Kit+/copGFP knock-in mutation strain expresses the copGFP variant of Green Fluorescent Protein (from the copepod Pontellina plumata) from the endogenous Kit locus. copGFP fluorescence mimics the endogenous Kit expression pattern. This mutant mouse strain may be useful for fluorescent visualization of KIT+ cells.
Kenton M. Sanders, University of Nevada School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Kit | KIT proto-oncogene receptor tyrosine kinase |
Mice that are heterozygous for this alelle are viable, normal in size and do not display any gross behavioral abnormalities. Heterozygotes exhibit white patches at the end of the tail, the belly and paws. Homozygotes exhibit an embryonic lethal phenotype. Mutant allele expression levels (mRNA) are similar to endogenous Kit expression in multiple tissues as detected by qPCR analysis (brain, heart, liver, lung, kidney, stomach, small intestine, colon). High expression levels are detected in lung. copGFP fluorescence is detected in KIT immuno-positive gastrointestinal interstitial cells of Cajal (ICC) by immunohistochemical and flow cytometric analysis.
When crossed with mice homozygous for the Lepob allele (see Stock No. 000632), loss in the intramuscular and myenteric populations of ICC in the intestine and colon can be visualized with confocal microscopy.
The P1 artificial chromosome (PAC) clone 554-D1, containing exons 1-4 and a portion of intron 4 of the Kit gene, was modified by inserting copGFP (from the copepod Pontellina plumata), a Hprt-PGK cassette and the SV40 polyA signal, downstream of the start codon. The construct was electroporated into 129S7/SvEvBrd-Hprtb-m2 derived AB2.2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting male chimeric animals were crossed to C57BL/6J female mice, and then backcrossed to the same for approximately 10 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | GFP fluorescence is detected in KIT immuno-positive gastrointestinal interstitial cells of Cajal (ICC) by immunohistochemical and flow cytometric analysis. |
Allele Name | targeted mutation 1, Kenton M Sanders and Seungil Ro |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Kit+/copGFP; KitcopGFP; Kittm1(copGFP)Rosan |
Gene Symbol and Name | Kit, KIT proto-oncogene receptor tyrosine kinase |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP fluorescence is detected in KIT immuno-positive gastrointestinal interstitial cells of Cajal (ICC) by immunohistochemical and flow cytometric analysis. |
Strain of Origin | 129S7/SvEvBrd-Hprtb-m2 |
Chromosome | 5 |
Molecular Note | The P1 artificial chromosome (PAC) clone 554-D1, containing exons 1-4 and a portion of intron 4 of the Kit gene, was modified by insertion of copGFP (from the copepod Pontellina plumata), a Hprt-PGK cassette, and the SV40 polyA signal downstream of the start codon. |
When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes are not viable.
When using the B6.129S7-Kittm1Rosay/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #015813 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Kit<tm1Rosay> |
Frozen Mouse Embryo | B6.129S7-Kit<tm1Rosay>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S7-Kit<tm1Rosay>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S7-Kit<tm1Rosay>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S7-Kit<tm1Rosay>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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