CD4-PLZF transgenic mice contain CD4 promoter/enhancer elements driving expression of the zinc finger and BTB domain containing 16 (Zbtb16 or PLZF) cDNA from purified natural killer T (NKT cells). These mice may be useful for studying the function of PLZF in T cells.
Albert Bendelac , University of Chicago
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
CD4-PLZF transgenic mice contain CD4 promoter/enhancer elements driving expression of the zinc finger and BTB domain containing 16 (Zbtb16 or PLZF) cDNA from purified natural killer T (NKT cells). Hemizygous mice are viable and fertile. PLZF is a transcriptional regulator that is expressed in NKT cells and is required for their maturation into effector-type lymphocytes. Transgenic expression of PLZF using the CD4 promoter induces
conventional CD4 T cells to acquire effector characteristics. While
CD4 T cells are present at normal levels in the thymus, spleen, liver,
and lungs of the CD4-PLZF mouse, they are nearly absent in the blood
and lymph nodes. Greater than 98% of mature CD4 T cells display a
CD44hi/CD62Llo effector phenotype. An increased percentage of CD4 T cells produce both interleukin-4 (IL-4) and interferon (IFN) γ upon TCR stimulation. These mice may be useful for studying the function
of PLZF in T cells.
The CD4-PLZF transgene was designed with murine CD4 promoter/enhancer and intronic silencer elements driving expression of the zinc finger and BTB domain containing 16 (Zbtb16 or PLZF) gene. PLZF cDNA was isolated from purified C57BL/6 natural killer T (NKT) cells. The construct was injected into fertilized C57BL/6 oocytes. Germ line transmission of the transgene was confirmed and mice from founder line 1797 were backcrossed to C57BL/6 mice. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | Zbtb16, zinc finger and BTB domain containing 16, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 1797, Albert Bendelac |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | CD4p-PLZF |
Gene Symbol and Name | Tg(Cd4-Zbtb16)1797Aben, transgene insertion 1797, Albert Bendelac |
Gene Synonym(s) | |
Promoter | Cd4, CD4 antigen, mouse, laboratory |
Expressed Gene | Zbtb16, zinc finger and BTB domain containing 16, mouse, laboratory |
Strain of Origin | C57BL/6 |
Chromosome | UN |
General Note | Transgenic lines were derived from two transgenic founder mice and designated line 1797 and line 1960. No apparent differences were observed between the two lines. |
Molecular Note | The CD4-PLZF transgene was designed with murine CD4 promoter/enhancer and intronic silencer elements driving expression of the zinc finger and BTB domain containing 16 (Zbtb16 or PLZF) gene. Line 1797 was established. |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or to C57BL/6J (Stock No. 000664) inbred mice.
When using the C57BL/6-Tg(Cd4-Zbtb16)1797Aben/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #014644 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(Cd4-Zbtb16)1797Aben |
Frozen Mouse Embryo | C57BL/6-Tg(Cd4-Zbtb16)1797Aben/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Cd4-Zbtb16)1797Aben/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Cd4-Zbtb16)1797Aben/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(Cd4-Zbtb16)1797Aben/J | $3373.50 |
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