These ENU-induced Kdelr1 mutant mice exhibit hypopigmentation, reduced thymic cellularity and reduced percentages of CD4+ and CD8+ T cells in the blood. This mutant mouse strain may be useful in studies of T cell development and protein trafficking.
Bruce Beutler, University of Texas Southwestern Medical
Homozygotes: Mice that are homozygous for the mutation are viable, fertile, normal in size. Mice exhibit hypopigmentation, reduced thymic cellularity and reduced percentages of CD4+ and CD8+ T cells in the blood. CD8+ T cells display a high expression of the CD44 marker typically expressed on activated and memory T cells. This mutant mouse strain may be useful in studies of T cell development and protein trafficking.
For additional information, see The Scripps Research Institute Mutagenix website.
This missense point mutation was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males. Mutagenized males were outcrossed to C57BL/6J females. The mutation results in a A to G transversion at position 644 in exon 4 of 5 exons. The mutation alters the corresponding amino acid from tyrosine to cysteine at codon 158. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
|Allele Name||mutation 1, Bruce Beutler|
|Allele Type||Chemically induced (ENU) (Null/Knockout)|
|Allele Synonym(s)||daniel gray; Kdelr1dgy|
|Gene Symbol and Name||Kdelr1, KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1|
|Strain of Origin||C57BL/6J|
|Molecular Note||The mutation in the fourth exon corresponds to an A to G transition at nucleotide position 644 of the transcript that is predicted to replace tyrosine with cysteine at amino acid position 158 of the protein (Y158C).|
When maintaining a live colony, homozygous mice may be bred together.
When using the daniel gray mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #34372 in your Materials and Methods section.