These ENU-induced Atp11c mutant mice exhibit reduced numbers of CD19+ B cells and hyperbilirubinemia with elevated levels of cholic acid in the serum. This mutant mouse strain may be useful in studies of B cell lymphopoiesis and cholestasis.
Bruce Beutler, University of Texas Southwestern Medical
Mice that are homozygous for the mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. However, more than half of all homozygous females suffer dystocia after pregnancy. Mice exhibit reduced numbers of CD19+ B cells. The reduction in B cells is first observed at the pre-pro B to pro-B transition in the bone marrow. Marginal zone B cells persist in normal numbers. The donating investigator reports that mice develop hyperbilirubinemia with elevated levels of cholic acid in the serum. This mutant mouse strain may be useful in studies of B cell lymphopoiesis and cholestasis.
For additional information, see The Scripps Research Institute Mutagenix website.
This nonsense point mutation was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males. Mutagenized males were outcrossed to C57BL/6J females. The mutation results in a C to T transversion at position 2113 of the Atp11c transcript. The mutation alters the corresponding amino acid from glutamine to stop conversion at codon 655. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
|Allele Name||mutation 1, Bruce Beutler|
|Allele Type||Chemically induced (ENU)|
|Allele Synonym(s)||mutation 1, Bruce Beutler; Atp11cm1Btlr|
|Gene Symbol and Name||Atp11c, ATPase, class VI, type 11C|
|Gene Synonym(s)||ATPIQ; Atp11c-ps1; expressed sequence AI315324; A330005H02Rik; RIKEN cDNA A330005H02 gene; A330005H02Rik; ATPIG; Ig; RGD1564481; AI315324; HACXL|
|Strain of Origin||C57BL/6J|
|Molecular Note||The mutation corresponds to a C to T transition at nucleotide position 2113 of the transcript that replaces a triplet encoding glutamine to a termination codon at amino acid position 655 (E655Ter).|
The donating investigator maintains these mice as heterozygotes. Homozygous females are fertile, but have a high incidence of dystocia.
When using the emptyhive mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #34370 in your Materials and Methods section.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.