In this knockout strain, maintained on a mixed C57BL/6 x 129S6 background, a neomycin (neo) resistance cassette replaces exons 5-6 of the (Nr1h2) gene, abolishing gene function. These mice may be useful for studying lipid and cholesterol metabolism, and the regulation of the immune response.
David Mangelsdorf, UT Southwestern Medical Center
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Nr1h2 | nuclear receptor subfamily 1, group H, member 2 |
In this strain, maintained on a mixed C57BL/6 x 129S6 background, a neomycin (neo) resistance cassette replaces exons 5-6 of the nuclear receptor subfamily 1, group H, member 2 (Nr1h2) gene, abolishing gene function. Homozygous are viable, fertile, and normal in size. Also known as liver X receptor beta (Lxrβ), Nr1h2 binds to retinoid X receptors (RXRα, RXRβ, RXRγ; NR2B1, NR2B2, and NR2B3) and is activated by oxysterols to regulate the expression of genes involved in cholesterol metabolism. Homozygous Lxrβ-/- mice lose their ability to appropriately regulate cholesterol, lipid, and carbohydrate metabolism, and have defects in immune function. These defects are more severe when combined with the knockout of Lxrα (available as Stock No. 013763). These mice may be useful for studying lipid and cholesterol metabolism, and the regulation of the immune response.
A targeting vector was designed to replace exons 5-6 encoding DNA-binding domain and the ligand-binding domains of the nuclear receptor subfamily 1, group H, member 2 (Nr1h2) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric mice were bred to C57BL/6 mice. These mice were intercrossed and maintained on a mixed C57BL/6 x 129S6 background. Upon arrival at The Jackson Laboratory, mice were bred to B6129SF1/J (Stock No. 101043) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, David J Mangelsdorf |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | LXRbeta- |
Gene Symbol and Name | Nr1h2, nuclear receptor subfamily 1, group H, member 2 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 7 |
Molecular Note | Insertion of a targeting vector replaced exons 5 and 6 with a neomycin resistance cassette creating a null allele. Exons 5 and 6 encode the DNA-binding and ligand-binding domains. |
Mutations Made By | David Mangelsdorf, UT Southwestern Medical Center |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6;129S-Nr1h2tm1Djm/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #014635 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Nr1h2<tm1Djm> |
Frozen Mouse Embryo | B6;129S-Nr1h2<tm1Djm>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S-Nr1h2<tm1Djm>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S-Nr1h2<tm1Djm>/J | $3373.50 |
Frozen Mouse Embryo | B6;129S-Nr1h2<tm1Djm>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.