B6;129S-Nr1h2^tm1Djm/J

Stock number: 014635
Research areas: Cardiovascular Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Metabolism Research, Reproductive Biology Research

Description

In this knockout strain, maintained on a mixed C57BL/6 x 129S6 background, a neomycin (neo) resistance cassette replaces exons 5-6 of the (Nr1h2) gene, abolishing gene function. These mice may be useful for studying lipid and cholesterol metabolism, and the regulation of the immune response.

Detailed description

In this strain, maintained on a mixed C57BL/6 x 129S6 background, a neomycin (neo) resistance cassette replaces exons 5-6 of the nuclear receptor subfamily 1, group H, member 2 (Nr1h2) gene, abolishing gene function. Homozygous are viable, fertile, and normal in size. Also known as liver X receptor beta (Lxrβ), Nr1h2 binds to retinoid X receptors (RXRα, RXRβ, RXRγ; NR2B1, NR2B2, and NR2B3) and is activated by oxysterols to regulate the expression of genes involved in cholesterol metabolism. Homozygous Lxrβ^-/- mice lose their ability to appropriately regulate cholesterol, lipid, and carbohydrate metabolism, and have defects in immune function. These defects are more severe when combined with the knockout of Lxrα (available as Stock No. 013763). These mice may be useful for studying lipid and cholesterol metabolism, and the regulation of the immune response.

Development

A targeting vector was designed to replace exons 5-6 encoding DNA-binding domain and the ligand-binding domains of the nuclear receptor subfamily 1, group H, member 2 (Nr1h2) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric mice were bred to C57BL/6 mice. These mice were intercrossed and maintained on a mixed C57BL/6 x 129S6 background. Upon arrival at The Jackson Laboratory, mice were bred to B6129SF1/J (Stock No. 101043) for at least one generation to establish the colony.

Allele

Symbol: Nr1h2^tm1Djm
Name: targeted mutation 1, David J Mangelsdorf
MGI accession ID: MGI:3041856
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Nr1h2
Marker name: nuclear receptor subfamily 1, group H, member 2
Chromosome: 7
Strain of origin: 129S6/SvEvTac
Molecular note: Insertion of a targeting vector replaced exons 5 and 6 with a neomycin resistance cassette creating a null allele. Exons 5 and 6 encode the DNA-binding and ligand-binding domains.