These mice harbor a knock-in mutation designed to insert an EGFP sequence in the 3' UTR of the Mecp2 gene. These mice may be useful for visualizing MECP2-expressing neuronal cells.
Adrian Bird, University of Edinburgh
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter) | Mecp2 | methyl CpG binding protein 2 |
A targeted mutation was designed to insert an enhanced green fluorescent protein sequence (EGFP) sequence in the 3' UTR of the methyl CpG binding protein 2 (Mecp2) gene. Homozygous females and male carriers are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Mecp2 is located on the X-chromosome, and exhibits increased expression in the central nervous system during neuronal maturation. Mutations in the Mecp2 gene can cause Rett Syndrome, an autism-spectrum neurodevelopmental disorder. Under control of the endogenous Mecp2 promoter, these mice express EGFP in neurons throughout the brain at levels consistent with endogenous MECP2 expression in wildtype mice from the colony. These mice may be useful for visualizing MECP2 expressing neuronal cells.
A targeting construct was designed to insert an enhanced green fluorescent protein sequence (EGFP) sequence and a loxP-flanked Neomycin (neo) resistance sequence-STOP cassette in the 3' UTR of the methyl CpG binding protein 2 (Mecp2) gene. This construct was electroporated into 129P2/OlaHsd-derived E14TG2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with B6.C-Tg(CMV-cre)1Cgn/J mice (Stock No. 006054) to remove the floxed-neo-STOP cassette. Mice lacking both the floxed sequence and the CMV-cre transgene were subsequently backcrossed to C57BL/6 for at least 2 generations prior to arrival at The Jackson Laboratory. Upon arrival, these Mecp2-EGFP mutant mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | EGFP is expressed in neurons throughout the brain. |
Allele Name | targeted mutation 3.1, Adrian Bird |
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Allele Type | Targeted (Reporter) |
Allele Synonym(s) | MECP2-EGFP |
Gene Symbol and Name | Mecp2, methyl CpG binding protein 2 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP is expressed in neurons throughout the brain. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | X |
Molecular Note | A targeting construct was designed to insert an enhanced green fluorescent protein sequence (EGFP) sequence (preceded by sequence for linker peptide CKDPPVAT) and a loxP-flanked neomycin (neo) resistance sequence-STOP cassette in the 3' UTR. Subsequent cre-mediated recombination removed the floxed neo-STOP cassette. This allele expresses a chimeric protein with the EGFP peptide linked to the C-terminus of the endogenous peptide. |
When maintaining a live colony, homozygous females may be bred to hemizygous males. Note: Mutation is X-linked.
When using the STOCK Mecp2tm3.1Bird/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #014610 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
X linked - Heterozygous females or Wild-type males for Mecp2<tm3.1Bird> |
Frozen Mouse Embryo | STOCK Mecp2<tm3.1Bird>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Mecp2<tm3.1Bird>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Mecp2<tm3.1Bird>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Mecp2<tm3.1Bird>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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