This mutant mouse strain carrying a targeted mutation of Lum, lumican, exhibits delayed wound healing and fragile skin. These mutant mice may be useful in studies of collagen fibrillogenesis and matrix organization, extracellular matrix, corneal inflammatory response, and epithelial wound healing, including neutrophil recruitment and invasion.
Winston W.-Y. Kao, University of Cincinnati
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Lum | lumican |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Male mice homozygous for this targeted mutation on the C57BL/6 background produce fewer pups than homozygous females when mated to wildtype mice. No gene product (mRNA or protein) is detected by Northern blot, in situ hybridization, and immunohistochemical analysis of eye tissue from homozygotes.
Homozygotes exhibit fragile skin, disarranged back skin hair, age related progressive corneal opacity and delayed corneal epithelial wound healing. Although the neutrophil infiltration response to lipopolysaccharide (LPS)-induced corneal keratitis in homozygous mutant mice is diminished when compared to wildtype controls, corneal haze is more pronounced. Keratocan levels in corneas from homozygotes is reduced compared to wildtype controls. Ultrastructural examination of the sclera from homozygotes reveals abnormal scleral morphology with less organized lamellae, collagen fibrils of larger diameter, and disorganized interfibrillar spacing. Sclera from heterozygotes exhibit an intermediate structural phenotype.
A targeting vector containing a PGK-Hprt selection cassette was used to disrupt exon 2. The construct was electroporated into 129P2/OlaHsd derived E14TG2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice. Then donating investigator reports that these mice were backcrossed to C57BL/6 for 10 generations (see SNP note below). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. Eleven of the 27 markers throughout the genome were found to be segregating. All 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed genetic background.
Allele Name | targeted mutation 1, Winston W Y Kao |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | lum-; Lum- |
Gene Symbol and Name | Lum, lumican |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 10 |
Molecular Note | The gene was disrupted by replacement of 481 bp of exon 2 with a PGK-hprt cassette (in the opposite orientation) via homologous recombination. Northern blot, in situ hybridization, and immunohistochemistry confirmed the absence of mRNA and protein expression in eyes of homozygous mutant animals. |
Mutations Made By | Winston W.-Y. Kao, University of Cincinnati |
When maintaining a live colony, these mice are bred as homozygous females crossed to heterozygous males. Male mice homozygous for this targeted mutation on the C57BL/6 background produce fewer pups than homozygous females when mated to wildtype mice.
When using the B6;129P2-Lumtm1Wwk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #014558 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Lum<tm1Wwk> |
Frozen Mouse Embryo | B6;129P2-Lum<tm1Wwk>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Lum<tm1Wwk>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Lum<tm1Wwk>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Lum<tm1Wwk>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.