Overview

Also Known As:B6.Cg-Hprt^{tm75(Ple143-lacZ)Ems}

These mice have the Ple143-lacZ transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. The promoter/regulatory regions of the human NR2F2 gene direct expression of β-galactosidase.

Donating Investigator

Elizabeth M Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia

Genetic overview

Genetic Background	Generation

Hprt^{tm75(Ple143-lacZ)Ems}

Allele Type	Gene Symbol	Gene Name
Targeted	Hprt	hypoxanthine guanine phosphoribosyl transferase

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Research Applications

Research Tools
Neurobiology Research

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Details

Detailed Description

These Ple143-lacZ;mEMS4990 mice have the Ple143-lacZ transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the nuclear receptor subfamily 2, group F, member 2 [NR2F2] gene directing expression of β-galactosidase (lacZ). These Ple143-lacZ;mEMS4990 mice may be useful in studying NR2F2-expressing cells in the brain and diseases affecting the brain.

For Ple143-lacZ;mEMS4990 expression information, see The Pleiades Promoter Project website (Ple143 Promoter (bEMS85)).

The lacZ expression pattern is described by the donating investigator as:

These mice were created and deposited by The Pleiades Promoter Project (Centre for Molecular Medicine and Therapeutics, University of British Columbia).

Development

The Ple143-lacZ transgene (bEMS85) was designed with the Ple143 MaxiPromoter (derived from the BAC RP11-134D15 that contains the whole NR2F2 gene plus ~117kb upstream and ~80kb downstream sequences) upstream of a frt-flanked β-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome via electroporation into mEMS1292 embryonic stem cells (a male ES cell line derived from a B6129F1-A^w-J/A^w Gt(ROSA)26Sor^tm1Sor/+ Hprt^b-m3/Y mouse (F1 generation male pups born of C57BL/6J-congenic females homozygous for the A^w-J and Hprt^b-m3 mutations [Hprt^b-m3 was originally derived from 129P2/OlaHsd-derived E14TG2a embryonic stem cells] X 129S-Gt(ROSA)26Sor^tm1Sor heterozygous males (see Stock No. 000051, 002171 and 003310, respectively)). Correctly targeted embryonic stem cells were microinjected into recipient mice cells. The resulting chimeric males (founder line mEMS4990) were bred to B6(Cg)-Tyr^c-2J/J females (B6-albino; Stock No. 000058) to establish the mutant colony. The donating investigator reports that these Ple143-lacZ;mEMS4990 mutant mice were bred with C57BL/6J wildtype mice for six generations prior to sending to The Jackson Laboratory. Upon arrival, Ple143-lacZ;mEMS4990 mutant males were used to cryopreserve sperm. When rederiving a live colony, sperm will be used along with oocytes from C57BL/6J inbred mice (Stock No. 000664) to generate obligate heterozygous females, and these females will be bred at least one generation to C57BL/6J inbred mice to establish the colony.

For more information, see The Pleiades Promoter Project website (Ple143 Promoter (bEMS85)).

Expression Data

Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	lacZ is expressed in areas of the brain.

Selected References

The Pleiades Promoter Project. 2009. Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain MGI Direct Data SubmissionMGI: J:145689

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Genetics

Hprt^{tm75(Ple143-lacZ)Ems}

Allele Symbol: Hprt^{tm75(Ple143-lacZ)Ems}

Allele Name	targeted mutation 75, Elizabeth M Simpson
Allele Type	Targeted
Allele Synonym(s)	Hprt^{tm75(mEMS4990)Ems}; NR2F2-lacZ; Ple143
Gene Symbol and Name	Hprt, hypoxanthine guanine phosphoribosyl transferase
Gene Synonym(s)
Promoter	NR2F2, nuclear receptor subfamily 2 group F member 2, human
Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	lacZ is expressed in areas of the brain.
Strain of Origin	(B6.Cg-A^w-J Hprt^b-m3/Ems x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Chromosome	X
General Note	Germ line transmission of mutant cell line mEMS4990 has been established.
Molecular Note	The Ple143-lacZ transgene (bEMS85) was designed with the Ple143 MaxiPromoter (derived from the BAC RP11-134D15 that contains the whole NR2F2 gene plus 117kb upstream and 80kb downstream sequences) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome.
Mutations Made By	Elizabeth Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Neurobiology Research
  - cell marker
- Genetics Research
  - Tissue/Cell Markers: neurons
  - Tissue/Cell Markers
- lacZ Expression
Neurobiology Research
- lacZ expression in neural tissue

Mammalian Phenotype Terms by Genotype

References

The Pleiades Promoter Project. 2009. Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain MGI Direct Data SubmissionMGI: J:145689

Additional - Hprt^{tm75(Ple143-lacZ)Ems} related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Probe:Gt(rosa)26sor Probe
- Genotyping resources and troubleshooting
Breeding Considerations

The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males.
- Additional Breeding and Husbandry Support
Citation
When using the B6.Cg-Hprt^{tm75(Ple143-lacZ)Ems}/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32966 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:B6.Cg-Hprttm75(Ple143-lacZ)Ems

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Expression Data

Selected References

Hprttm75(Ple143-lacZ)Ems

Allele Symbol: Hprttm75(Ple143-lacZ)Ems

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Hprttm75(Ple143-lacZ)Ems related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Also Known As:B6.Cg-Hprt^{tm75(Ple143-lacZ)Ems}

Hprt^{tm75(Ple143-lacZ)Ems}

Allele Symbol: Hprt^{tm75(Ple143-lacZ)Ems}

Additional - Hprt^{tm75(Ple143-lacZ)Ems} related