B6.Cg-Hprt^{tm75(Ple143-lacZ)Ems}/Mmjax

Stock number: 014536
Strain synonyms: B6.Cg-Hprt^{tm75(Ple143-lacZ)Ems}
Research areas: Neurobiology Research, Research Tools

Description

These mice have the Ple143-lacZ transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. The promoter/regulatory regions of the human NR2F2 gene direct expression of β-galactosidase.

Detailed description

These Ple143-lacZ;mEMS4990 mice have the Ple143-lacZ transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the nuclear receptor subfamily 2, group F, member 2 [NR2F2] gene directing expression of β-galactosidase (lacZ). These Ple143-lacZ;mEMS4990 mice may be useful in studying NR2F2-expressing cells in the brain and diseases affecting the brain.

For Ple143-lacZ;mEMS4990 expression information, see The Pleiades Promoter Project website (Ple143 Promoter (bEMS85)).

The lacZ expression pattern is described by the donating investigator as:

These mice were created and deposited by The Pleiades Promoter Project (Centre for Molecular Medicine and Therapeutics, University of British Columbia).

Development

The Ple143-lacZ transgene (bEMS85) was designed with the Ple143 MaxiPromoter (derived from the BAC RP11-134D15 that contains the whole NR2F2 gene plus ~117kb upstream and ~80kb downstream sequences) upstream of a frt-flanked β-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome via electroporation into mEMS1292 embryonic stem cells (a male ES cell line derived from a B6129F1-A^w-J/A^w Gt(ROSA)26Sor^tm1Sor/+ Hprt^b-m3/Y mouse (F1 generation male pups born of C57BL/6J-congenic females homozygous for the A^w-J and Hprt^b-m3 mutations [Hprt^b-m3 was originally derived from 129P2/OlaHsd-derived E14TG2a embryonic stem cells] X 129S-Gt(ROSA)26Sor^tm1Sor heterozygous males (see Stock No. 000051, 002171 and 003310, respectively)). Correctly targeted embryonic stem cells were microinjected into recipient mice cells. The resulting chimeric males (founder line mEMS4990) were bred to B6(Cg)-Tyr^c-2J/J females (B6-albino; Stock No. 000058) to establish the mutant colony. The donating investigator reports that these Ple143-lacZ;mEMS4990 mutant mice were bred with C57BL/6J wildtype mice for six generations prior to sending to The Jackson Laboratory. Upon arrival, Ple143-lacZ;mEMS4990 mutant males were used to cryopreserve sperm. When rederiving a live colony, sperm will be used along with oocytes from C57BL/6J inbred mice (Stock No. 000664) to generate obligate heterozygous females, and these females will be bred at least one generation to C57BL/6J inbred mice to establish the colony.

For more information, see The Pleiades Promoter Project website (Ple143 Promoter (bEMS85)).

Allele

Symbol: Hprt^{tm75(Ple143-lacZ)Ems}
Name: targeted mutation 75, Elizabeth M Simpson
MGI accession ID: MGI:5050094
Generation method: Targeted
Marker symbol: Hprt
Marker name: hypoxanthine guanine phosphoribosyl transferase
Chromosome: X
Strain of origin: (B6.Cg-A^w-J Hprt^b-m3/Ems x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ is expressed in areas of the brain.
Molecular note: The Ple143-lacZ transgene (bEMS85) was designed with the Ple143 MaxiPromoter (derived from the BAC RP11-134D15 that contains the whole NR2F2 gene plus 117kb upstream and 80kb downstream sequences) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome.
General note: Germ line transmission of mutant cell line mEMS4990 has been established.