These mice have the Ple143-lacZ transgene targeted as a single copy "knockin" into the upstream region of the Hprt locus on the X chromosome. The promoter/regulatory regions of the human NR2F2 gene direct expression of β-galactosidase.
Elizabeth M Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted | Hprt | hypoxanthine guanine phosphoribosyl transferase |
These Ple143-lacZ;mEMS4990 mice have the Ple143-lacZ transgene targeted as a single copy "knockin" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. Heterozygous females and hemizygous males are viable and fertile, with the promoter/regulatory regions of the nuclear receptor subfamily 2, group F, member 2 [NR2F2] gene directing expression of β-galactosidase (lacZ). These Ple143-lacZ;mEMS4990 mice may be useful in studying NR2F2-expressing cells in the brain and diseases affecting the brain.
For Ple143-lacZ;mEMS4990 expression information, see The Pleiades Promoter Project website (Ple143 Promoter (bEMS85)).
The lacZ expression pattern is described by the donating investigator as:
These mice were created and deposited by The Pleiades Promoter Project (Centre for Molecular Medicine and Therapeutics, University of British Columbia).
The Ple143-lacZ transgene (bEMS85) was designed with the Ple143 MaxiPromoter (derived from the BAC RP11-134D15 that contains the whole NR2F2 gene plus ~117kb upstream and ~80kb downstream sequences) upstream of a frt-flanked β-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome via electroporation into mEMS1292 embryonic stem cells (a male ES cell line derived from a B6129F1-Aw-J/Aw Gt(ROSA)26Sortm1Sor/+ Hprtb-m3/Y mouse (F1 generation male pups born of C57BL/6J-congenic females homozygous for the Aw-J and Hprtb-m3 mutations [Hprtb-m3 was originally derived from 129P2/OlaHsd-derived E14TG2a embryonic stem cells] X 129S-Gt(ROSA)26Sortm1Sor heterozygous males (see Stock No. 000051, 002171 and 003310, respectively)). Correctly targeted embryonic stem cells were microinjected into recipient mice cells. The resulting chimeric males (founder line mEMS4990) were bred to B6(Cg)-Tyrc-2J/J females (B6-albino; Stock No. 000058) to establish the mutant colony. The donating investigator reports that these Ple143-lacZ;mEMS4990 mutant mice were bred with C57BL/6J wildtype mice for six generations prior to sending to The Jackson Laboratory. Upon arrival, Ple143-lacZ;mEMS4990 mutant males were used to cryopreserve sperm. When rederiving a live colony, sperm will be used along with oocytes from C57BL/6J inbred mice (Stock No. 000664) to generate obligate heterozygous females, and these females will be bred at least one generation to C57BL/6J inbred mice to establish the colony.
For more information, see The Pleiades Promoter Project website (Ple143 Promoter (bEMS85)).
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | lacZ is expressed in areas of the brain. |
Allele Name | targeted mutation 75, Elizabeth M Simpson |
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Allele Type | Targeted |
Allele Synonym(s) | Hprttm75(mEMS4990)Ems; NR2F2-lacZ; Ple143 |
Gene Symbol and Name | Hprt, hypoxanthine guanine phosphoribosyl transferase |
Gene Synonym(s) | |
Promoter | NR2F2, nuclear receptor subfamily 2 group F member 2, human |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ is expressed in areas of the brain. |
Strain of Origin | (B6.Cg-Aw-J Hprtb-m3/Ems x 129S-Gt(ROSA)26Sortm1Sor/J)F1 |
Chromosome | X |
General Note | Germ line transmission of mutant cell line mEMS4990 has been established. |
Molecular Note | The Ple143-lacZ transgene (bEMS85) was designed with the Ple143 MaxiPromoter (derived from the BAC RP11-134D15 that contains the whole NR2F2 gene plus 117kb upstream and 80kb downstream sequences) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. |
Mutations Made By | Elizabeth Simpson, Centre for Molecular Medicine & Therapeutics, University of British Columbia |
The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males.
When using the B6.Cg-Hprttm75(Ple143-lacZ)Ems/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32966 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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