In these ENU-induced Tlr9 mutant mice, macrophages do not produce tumor necrosis factor (TNF) alpha in response to stimulation with oligodeoxynucleotides containing CpG motifs. This mutant mouse strain may be useful in studies of the role of toll like receptor 9 (TLR9) in the immune system.
Bruce Beutler, University of Texas Southwestern Medical
Genetic Background | Generation |
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?p+N2F6
|
Allele Type | Gene Symbol | Gene Name |
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Chemically induced (ENU) | Tlr9 | toll-like receptor 9 |
Homozygotes: Mice that are homozygous for this mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In response to stimulation with oligodeoxynucleotides containing CpG motifs, macrophages do not produce tumor necrosis factor (TNF) alpha. This mutant mouse strain may be useful in studies of the role of toll like receptor 9 (TLR9) in the immune system.
For additional information, see The Scripps Research Institute Mutagenix website.
Heterozygote: Macrophages from heterozygous mice produce a reduced level of TNF-alpha in response to CpG stimulation.
This missense point mutation was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males. Mutagenized males were outcrossed to C57BL/6J females. The mutation results in a T to C transition at position 1181 of the Tlr9 transcript. The mutation alters the corresponding amino acid from serine to proline at amino position 359 (S359P) in the eleventh leucine rich repeat. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Allele Name | mutation 7, Bruce Beutler |
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Allele Type | Chemically induced (ENU) |
Allele Synonym(s) | CpG11 |
Gene Symbol and Name | Tlr9, toll-like receptor 9 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 9 |
Molecular Note | This mutation, discovered in a screen of progeny of ethylnitrosourea (ENU) mutagenized mice for impaired responses to Toll-like receptor (TLR) ligands, has been identified as a T to C transition at nucleotide position 1181 of the transcript that is predicted to result in the replacement of serine by proline at amino acid position 359 of the protein (S359P), in the second leucine rich repeat. |
Mutations Made By | Bruce Beutler, University of Texas Southwestern Medical |
While maintaining a live colony, these mice are bred as homozygotes.
When using the CpG11 mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #34329 in your Materials and Methods section.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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