Overview

Also Known As:CpG11

In these ENU-induced Tlr9 mutant mice, macrophages do not produce tumor necrosis factor (TNF) alpha in response to stimulation with oligodeoxynucleotides containing CpG motifs. This mutant mouse strain may be useful in studies of the role of toll like receptor 9 (TLR9) in the immune system.

Donating Investigator

Bruce Beutler, University of Texas Southwestern Medical

Genetic overview

Genetic Background	Generation

Tlr9^M7Btlr

Allele Type	Gene Symbol	Gene Name
Chemically induced (ENU)	Tlr9	toll-like receptor 9

View Genetics

Research Applications

Immunology, Inflammation and Autoimmunity Research

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Details

Detailed Description

Homozygotes: Mice that are homozygous for this mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. In response to stimulation with oligodeoxynucleotides containing CpG motifs, macrophages do not produce tumor necrosis factor (TNF) alpha. This mutant mouse strain may be useful in studies of the role of toll like receptor 9 (TLR9) in the immune system.

For additional information, see The Scripps Research Institute Mutagenix website.

Heterozygote: Macrophages from heterozygous mice produce a reduced level of TNF-alpha in response to CpG stimulation.

Development

This missense point mutation was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males. Mutagenized males were outcrossed to C57BL/6J females. The mutation results in a T to C transition at position 1181 of the Tlr9 transcript. The mutation alters the corresponding amino acid from serine to proline at amino position 359 (S359P) in the eleventh leucine rich repeat. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Huang H; Beutler B. 2010. CpG11 is an allele of Tlr9 and causes Tlr9 signaling defects MGI Direct Data SubmissionMGI: J:165701

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Genetics

Tlr9^M7Btlr

Allele Symbol: Tlr9^M7Btlr

Allele Name	mutation 7, Bruce Beutler
Allele Type	Chemically induced (ENU)
Allele Synonym(s)	CpG11
Gene Symbol and Name	Tlr9, toll-like receptor 9
Gene Synonym(s)
Strain of Origin	C57BL/6J
Chromosome	9
Molecular Note	This mutation, discovered in a screen of progeny of ethylnitrosourea (ENU) mutagenized mice for impaired responses to Toll-like receptor (TLR) ligands, has been identified as a T to C transition at nucleotide position 1181 of the transcript that is predicted to result in the replacement of serine by proline at amino acid position 359 of the protein (S359P), in the second leucine rich repeat.
Mutations Made By	Bruce Beutler, University of Texas Southwestern Medical

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
- Immunodeficiency
  - Tlr deficiency
- Inflammation
  - Tlr deficiency
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
  - Tlr deficiency

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Tlr9^M7Btlr/Tlr9⁺
C57BL/6J-Tlr9

immune system phenotype

decreased tumor necrosis factor secretion
- upon stimulation with oligodeoxynucleotides containing CpG motifs (CpG ODNs), a Toll-like receptor 9 ligand, peritoneal macrophages from mice heterozygous for this mutation produce levels of tumor necrosis factor (TNF-) alpha intermediate between those of wild-type and homozygous mutant mice
- wild-type and homozygous mutant mice

Genotype: Tlr9^M7Btlr/Tlr9^M7Btlr
C57BL/6J-Tlr9

immune system phenotype

decreased tumor necrosis factor secretion
- in response to stimulation with oligodeoxynucleotides containing CpG motifs (CpG ODNs), a ligand for Toll-like receptor 9, peritoneal macrophages from mice homozygous for this mutation produce greatly reduced levels (vs. wild-type macrophages) of tumor necrosis factor (TNF-) alpha
- Normal - in contrast to their impaired response to a TLR9 ligand, homozygous mutant macrophages produce normal amounts of TNF-alpha upon stimulation with all other Toll-like receptor (TLR) ligands employed in the TLR Signaling Screen (for TLR1/2, TLR3, TLR4, TLR2/6 and TLR7)
- crosis factor (TNF-) alpha
- Normal - 6 and TLR7)

References

Huang H; Beutler B. 2010. CpG11 is an allele of Tlr9 and causes Tlr9 signaling defects MGI Direct Data SubmissionMGI: J:165701

Additional - Tlr9^M7Btlr related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Sanger sequencing:Tlr9-SEQ
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these mice are bred as homozygotes.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the CpG11 mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #34329 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:CpG11

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Tlr9M7Btlr

Allele Symbol: Tlr9M7Btlr

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Tlr9M7Btlr/Tlr9+C57BL/6J-Tlr9

immune system phenotype

Genotype: Tlr9M7Btlr/Tlr9M7BtlrC57BL/6J-Tlr9

immune system phenotype

References

Additional - Tlr9M7Btlr related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Tlr9^M7Btlr

Allele Symbol: Tlr9^M7Btlr

Genotype: Tlr9^M7Btlr/Tlr9⁺
C57BL/6J-Tlr9

Genotype: Tlr9^M7Btlr/Tlr9^M7Btlr
C57BL/6J-Tlr9

Additional - Tlr9^M7Btlr related