Mice homozygous for the brachypodism 4 Jackson have normal body length but the digits and all four limbs are shortened. Males have reduced fertility.
The Gdf5bp-4J mutation arose spontaneously in the homozygous B6.129S-Casp6tm1Flv/J strain at The Jackson Laboratory in 2008. To remove the targeted mutation from this mutant subline a Gdf5bp-4J female mutant was backcrossed to C57BL/6J and the offspring were intercrossed. Their offspring were genotyped for Casp6tm1Flv and those that were wild-type for Casp6 were selected to continue the line. This backcross and intercross was repeated once and this strain is now maintained by sibling breeding Gdf5bp-4J homozygous females to heterozygous males. In 2012 this mutant subline reached generation N2F2.
|Allele Name||brachypodism 4 Jackson|
|Gene Symbol and Name||Gdf5, growth differentiation factor 5|
|Strain of Origin||B6.129S6-Casp6tm1Flv/J|
|Molecular Note||Failed complementation test with either the Gdf5 |
Because homozygous males are not reliable breeders, this strain is maintained by breeding homozygous females with heterozygous males.
When using the B6.Cg-Gdf5bp-4J/GrsrJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #014183 in your Materials and Methods section.