This Irf8f floxed targeted mutation strain may be useful in generating conditional mutants for studying B cell differentiation, immunodeficiency (abnormal populations of B cells, T cells, granulocytes and macrophages), hematopoiesis, lymphoma and human chronic myeloid leukemia.
Herbert C. Morse III, Laboratory of Immunopathology, NIAID, NI
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Irf8 | interferon regulatory factor 8 |
These mice possess loxP sites on either side of exon 2, which encodes the DNA binding domain. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s).
When bred to a strain with Cre recombinase expression in B lymphocytes (see Stock No. 006785 for example), this mutant mouse strain may be useful in studies of B cell differentiation.
A targeting vector containing an FRT site flanked PGK-Neo selection cassette was utilized in the construction of this mutant. loxP sites were inserted upstream and downstream of exon 2. This construct was electroporated into C57BL/6 derived embryonic stem (ES) cells (Ozgene). Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to transgenic mice (on the C57BL/6 genetic background) expressing FLP recombinase. Mice that retained the loxP site flanked exon 2 were then bred to C57BL/6 mice to remove the FLP transgene. Heterozygotes were crossed to generate homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Allele Name | targeted mutation 1.1, Herbert Morse III |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Irf8f |
Gene Symbol and Name | Irf8, interferon regulatory factor 8 |
Gene Synonym(s) | |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 8 |
Molecular Note | Exon 2 was floxed. The FRT-flanked neo cassette used for selection was removed by flp-mediated recombination. |
Mutations Made By | Herbert Morse III, Laboratory of Immunopathology, NIAID, NI |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the Irf8f mouse strain in a publication, please cite the originating article(s) and include JAX stock #014175 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Irf8<tm1Hm> |
Frozen Mouse Embryo | B6(Cg)-Irf8<tm1.1Hm>/J | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Irf8<tm1.1Hm>/J | $2595.00 |
Frozen Mouse Embryo | B6(Cg)-Irf8<tm1.1Hm>/J | $3373.50 |
Frozen Mouse Embryo | B6(Cg)-Irf8<tm1.1Hm>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.