B6.Cg-Irf8^tm1.1Hm/J

Stock number: 014175
Product name: Irf8^f
Research areas: Cancer Research, Cell Biology Research, Developmental Biology Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools, Virology Research

Description

This Irf8^f floxed targeted mutation strain may be useful in generating conditional mutants for studying B cell differentiation, immunodeficiency (abnormal populations of B cells, T cells, granulocytes and macrophages), hematopoiesis, lymphoma and human chronic myeloid leukemia.

Detailed description

These mice possess loxP sites on either side of exon 2, which encodes the DNA binding domain. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue(s).

When bred to a strain with Cre recombinase expression in B lymphocytes (see Stock No. 006785 for example), this mutant mouse strain may be useful in studies of B cell differentiation.

Development

A targeting vector containing an FRT site flanked PGK-Neo selection cassette was utilized in the construction of this mutant. loxP sites were inserted upstream and downstream of exon 2. This construct was electroporated into C57BL/6 derived embryonic stem (ES) cells (Ozgene). Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to transgenic mice (on the C57BL/6 genetic background) expressing FLP recombinase. Mice that retained the loxP site flanked exon 2 were then bred to C57BL/6 mice to remove the FLP transgene. Heterozygotes were crossed to generate homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Irf8^tm1.1Hm
Name: targeted mutation 1.1, Herbert Morse III
MGI accession ID: MGI:4939593
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Irf8
Marker name: interferon regulatory factor 8
Chromosome: 8
Strain of origin: B6.Cg-Thy1^a
Molecular note: Exon 2 was floxed. The FRT-flanked neo cassette used for selection was removed by flp-mediated recombination.