These Dicer1c mutant mice possess loxP sites flanking exons 25-27 of the Dcr-1 homolog (Dicer1) gene. Mice that are homozygous for this allele still contain a frt-flanked neo and are viable and fertile. DICER1 is an interference RNA (RNAi) enzyme required by the small temporal RNA pathways to produce the active small RNA component that represses gene expression. This enzyme is required for many cellular processes, including oocyte development. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 25-27 deleted in the cre-expressing tissues.
For example, when bred to transgenic mice expressing Cre recombinase driven by the Zp3 (zona pellucida glycoprotein 3) promoter/enhanced elements (see Stock No. 003651 for example), oocyte mitosis is arrested at metaphase, leading to oocyte degradation and infertility.
When bred to a strain expressing Cre recombinase in embryonic primordial germ cells. (see Stock No. 008569 for example), this mutant mouse strain may be useful in studies of chromosome segregation and meiotic maturation.
A targeting vector was designed to insert a loxP site upstream of exon 25 and a second loxP site, followed by a frt-flanked neomycin resistance (neo) cassette, downstream of exon 27 of the Dcr-1 homolog (Dicer1) gene. The construct was electroporated into 129S1/SvImJ-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to 129S1 mice. Offspring heterozygous for this Dicer1 allele were bred with 129S1 mice to establish a Dicer1c colony. Upon arrival, mice were bred to 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation.
|Allele Name||targeted mutation 1, Jeff Mann|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Dicer1, dicer 1, ribonuclease type III|
|Strain of Origin||129S1/SvImJ|
|Molecular Note||A loxP site was inserted upstream of exon 25 and an frt flanked neo cassette with a 5' loxP site was inserted downstream of exon 27.|
When maintaining a live colony, homozygous mice may be bred together.
When using the 129S-Dicer1tm1Mnn/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #014167 in your Materials and Methods section.