This Tmem100-GCE transgenic strain expresses GFP and tamoxifen inducible Cre recombinase in the developing kidney and reproductive tracts and may be useful in studies of genitourinary development.
Andrew P McMahon, University of Southern California
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing, Reporter, Inducible) |
These transgenic mice express the eGFPCreERT2 (Enhanced Green Fluorescent Protein and cre/ESR1) fusion gene under the direction of the mouse Tmem100, transmembrane protein 100, promoter. Transgene expression is detected in developing nephrons of the kidney, Wolfian and Mullerian ducts and vasculature of the urogenital system of hemizygous 15.5 dpc embryos. GFP fluorescence is detected in embryos 15.5dpc in age in the proximal region of the early renal vesicle, at later stages eGFP is present in the parietal epithelium adjacent to Wilms Tumor (WT1) positive cells demarcating the future podocytes of Bowman's capsule and is limited to the Tmem100 expression domain. Tamoxifen inducible Cre recombinase activity is detected in the developing kidney (nephrogenic zone), vasculature and Wolfian or Mullerian duct of mutant embryos aged 15.5dpc. Possible cre recombinase activity is detected in small renal arteries. Other possible sites of expression have not been characterized. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
This strain was transferred from the collection of the GenitoUrinary Development Molecular Anatomy Project (GUDMAP).
A targeting construct containing the eGFPCreERT2 (GCE) cassette and FRT site flanked Kanamycin/neomycin selection cassette was inserted into the consensus start ATG codon of the gene in a BAC (RP23-183L17). The PGK-Neo/Kan cassette was removed by transient Flpe-recombinase expression. The resulting BAC transgene was microinjected into (C57BL/6 x DBA) F1donor eggs. Founder line 30 was subsequently established. The mice were then crossed to C57BL/6 for two generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | ESR1, estrogen receptor 1, human |
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Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | GFP and tamoxifen inducible cre recombinase is expressed in the developing kidney and reproductive tracts. |
Allele Name | transgene insertion 30, Andrew P McMahon |
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Allele Type | Transgenic (Recombinase-expressing, Reporter, Inducible) |
Allele Synonym(s) | TmemGCE; Tmem100-GCE |
Gene Symbol and Name | Tg(Tmem100-EGFP/cre/ERT2)30Amc, transgene insertion 30, Andrew P McMahon |
Gene Synonym(s) | |
Promoter | Tmem100, transmembrane protein 100, mouse, laboratory |
Expressed Gene | ESR1, estrogen receptor 1, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | GFP and tamoxifen inducible cre recombinase is expressed in the developing kidney and reproductive tracts. |
Strain of Origin | (C57BL/6 x DBA)F1 |
Chromosome | UN |
Molecular Note | A targeting construct containing the eGFPCreERT2 (GCE) cassette and Frt site flanked Kanamycin/neomycin selection cassette was inserted into the consensus start ATG codon of the gene in a BAC (RP23-183L17). The PGK-Neo/Kan cassette was removed by transient Flpe-recombinase expression. The resulting BAC transgene was microinjected into (C57BL/6 x DBA)F1 donor eggs. Founder line 30 was subsequently established. Native GFP expression is detectable in the urogenital system of E15.5 embryos. Transgenic cre expression is detected in developing nephrons of the kidney, Wolfian and Mullerian ducts and vasculature of the urogenital system of hemizygous 15.5 dpc embryos. Possible cre recombinase activity is detected in small renal arteries. |
Mutations Made By | Andrew McMahon, University of Southern California |
When maintaining a live colony, transgene carrier mice may be bred together, to wildtype (noncarrier) mice from the colony, or to C57BL/6J inbred mice.
When using the B6;DBA-Tg(Tmem100-EGFP/cre/ERT2)30Amc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #014159 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Tmem100-EGFP/cre/ERT2)30Amc |
Frozen Mouse Embryo | B6;DBA-Tg(Tmem100-EGFP/cre/ERT2)30Amc/J | $2595.00 |
Frozen Mouse Embryo | B6;DBA-Tg(Tmem100-EGFP/cre/ERT2)30Amc/J | $2595.00 |
Frozen Mouse Embryo | B6;DBA-Tg(Tmem100-EGFP/cre/ERT2)30Amc/J | $3373.50 |
Frozen Mouse Embryo | B6;DBA-Tg(Tmem100-EGFP/cre/ERT2)30Amc/J | $3373.50 |
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