Lkb1fl mice possess loxP sites flanking exons 3-6 of the serine/threonine kinase 11 (Stk11) gene. Exposure to Cre recombinase removes the floxed sequence - creating a null allele. This strain may be useful for studying cell growth and tumor suppression.
Sean J Morrison, University of Texas Southwestern Medical Center
These Lkb1fl mice possess loxP sites flanking exons 3-6 of the serine/threonine kinase 11 (Stk11) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Stk11 is a member of the serine/threonine kinase family and regulates cell polarity and cell division, and controls cell energy expenditure. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 3-6 deleted in the cre-expressing tissues.
For example, when bred to transgenic mice expressing Cre recombinase driven by myxovirus (influenza virus) resistance 1 (Mx1) promoter/enhanced elements, STK11 expression is abolished in hematopoietic cells. This strain may be useful for studying cell growth and tumor suppression.
Furthermore, when crossed to a strain expressing tamoxifen-inducible Cre recombinase (see Stock No. 008085) or interferon-inducible Cre recombinase (see Stock No. 003556), this mutant mouse strain may be useful in studies of metabolic and cell cycle regulation.
A targeting vector was designed to insert a loxP site followed by a Frt-flanked neomycin resistance cassette (neo) upstream of exon 3, and a second loxP site downstream of exon 6 of the serine/threonine kinase 11 (Stk11) gene. The construct was electroporated into Bruce4-derived C57BL/6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and the resulting chimeric males were bred to female B6(Cg)-Tyrc-2J/J mice (Stock No. 000058) to establish a colony. These mice were then bred to mice carrying Tg(ACTFLPe)9205Dym to remove the neo cassette. Offspring were then backcrossed to C57BL/6 for at least 4 generations (see SNP note below). Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. At least 11 the 32 markers on 5 chromosomes were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed genetic background.
|Allele Name||targeted mutation 1.1, Sean J Morrison|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Stk11, serine/threonine kinase 11|
|Strain of Origin||B6.Cg-Thy1a|
|Molecular Note||An FRT-flanked neo cassette with a 5'loxP site was inserted upstream of exon 3 and an additional loxP site was inserted downstream of exon 6. Flp mediated recombination removed the neo cassette.|
|Mutations Made By|| |
Sean Morrison, University of Texas Southwestern Medical Center
When maintaining a live colony, homozygous mice may be bred together.
When using the Lkb1fl mouse strain in a publication, please cite the originating article(s) and include JAX stock #014143 in your Materials and Methods section.