Removal of this mouse colony is imminent. If live mice are needed for your studies, it is advised that they be ordered immediately. After removal, the mice will be available from a cryorecovery.
Lkb1fl mice possess loxP sites flanking exons 3-6 of the serine/threonine kinase 11 (Stk11) gene. Exposure to Cre recombinase removes the floxed sequence - creating a null allele. This strain may be useful for studying cell growth and tumor suppression.
Sean J Morrison, University of Texas Southwestern Medical Center
These Lkb1fl mice possess loxP sites flanking exons 3-6 of the serine/threonine kinase 11 (Stk11) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Stk11 is a member of the serine/threonine kinase family and regulates cell polarity and cell division, and controls cell energy expenditure. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 3-6 deleted in the cre-expressing tissues.
For example, when bred to transgenic mice expressing Cre recombinase driven by myxovirus (influenza virus) resistance 1 (Mx1) promoter/enhanced elements, STK11 expression is abolished in hematopoietic cells. This strain may be useful for studying cell growth and tumor suppression.
Furthermore, when crossed to a strain expressing tamoxifen-inducible Cre recombinase (see Stock No. 008085) or interferon-inducible Cre recombinase (see Stock No. 003556), this mutant mouse strain may be useful in studies of metabolic and cell cycle regulation.
A targeting vector was designed to insert a loxP site followed by a Frt-flanked neomycin resistance cassette (neo) upstream of exon 3, and a second loxP site downstream of exon 6 of the serine/threonine kinase 11 (Stk11) gene. The construct was electroporated into Bruce4-derived C57BL/6 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and the resulting chimeric males were bred to female B6(Cg)-Tyrc-2J/J mice (Stock No. 000058) to establish a colony. These mice were then bred to mice carrying Tg(ACTFLPe)9205Dym to remove the neo cassette. Offspring were then backcrossed to C57BL/6 for at least 4 generations (see SNP note below). Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. At least 11 the 32 markers on 5 chromosomes were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed genetic background.
|Allele Name||targeted mutation 1.1, Sean J Morrison|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Stk11, serine/threonine kinase 11|
|Strain of Origin||B6.Cg-Thy1a|
|Molecular Note||An FRT-flanked neo cassette with a 5'loxP site was inserted upstream of exon 3 and an additional loxP site was inserted downstream of exon 6. Flp mediated recombination removed the neo cassette.|
|Mutations Made By|| |
Sean Morrison, University of Texas Southwestern Medical Center
When maintaining a live colony, homozygous mice may be bred together.
When using the Lkb1fl mouse strain in a publication, please cite the originating article(s) and include JAX stock #014143 in your Materials and Methods section.