Plasma dendritic cells from these ENU-induced Slc15a4 (feeble) mutant mice fail to produce IFN in response to challenge from CpG-A and TLR7 or TLR9 ligands. This mutant mouse strain may be useful in studies of toll like receptor signaling and plasmacytoid dendritic cell function in the immune system.
Bruce Beutler, University of Texas Southwestern Medical
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Chemically induced (ENU) | Slc15a4 | solute carrier family 15, member 4 |
Homozygotes: Mice that are homozygous for the mutation are viable and fertile. Although rare, plasmacytoid dendritic cells (pDCs) are responsible for most of the type 1 interferon (IFN) response following viral infection. pDCs from feeble mice fail to produce IFN in response to challenge from CpG-A and TLR7 or TLR9 ligands. In vitro challenge with TLR ligands abrogates secretion of IFN as well as that of other proinflammatory cytokines. In contrast, conventional dendritic cells from feeble mice produce a normal response to TLR ligands. This mutant mouse strain may be useful in studies of toll like receptor signaling and plasmacytoid dendritic cell function in the immune system.
For additional information, see The Scripps Research Institute Mutagenix website.
Heterozygote: Not evaluated;
heterozygote phenotype is expected to be normal.
This missense point mutation was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males. Mutagenized males were outcrossed to C57BL/6J females. The mutation results aberrant splicing due to a T to A transversion in the intron 2 donor splice site. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Allele Name | mutation 1, Bruce Beutler |
---|---|
Allele Type | Chemically induced (ENU) |
Allele Synonym(s) | feeble |
Gene Symbol and Name | Slc15a4, solute carrier family 15, member 4 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 5 |
Molecular Note | The feeble mutation has been identified as a T to A transversion in the intron 2 donor splice site. |
Mutations Made By | Bruce Beutler, University of Texas Southwestern Medical |
While maintaining a live colony, these mice are bred as homozygotes.
When using the C57BL/6J-Slc15a4m1Btlr/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #34296 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.