Fos/EGFP transgenic mice express a fusion gene consisting of the murine FBJ osteosarcoma oncogene (Fos) and enhanced green fluorescence protein (EGFP). These mice may be useful for visualizing Fos-expression in stimulated neurons, and for studying changes in neuron excitability and synaptic efficacy.
Alison Barth, Carnegie Mellon University
Genetic Background | Generation |
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N18+N2F1
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Allele Type |
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Transgenic (Reporter) |
Starting at:
$255.00 Domestic price for female 4-week |
333.51 Domestic price for breeder pair |
Fos/EGFP transgenic mice express a fusion gene consisting of the murine FBJ osteosarcoma oncogene (Fos) and enhanced green fluorescence protein (EGFP). Hemizygous Fos/EGFP mice are viable, fertile, and normal in size. Fos is a member of the activator protein-1 family of transcription factors and serves as a marker for neural activity. At basal state, Fos is expressed at low levels in the cerebellum, olfactory bulb, hippocampus, and neocortex. Fos expression is induced by a wide array of stimuli, including seizure, cortical injury, dehydration, and pain. In these mice, the Fos/EGFP fusion protein is expressed in recently activated neurons. These mice may be useful for visualizing Fos-expression in stimulated neurons, and for studying changes in neuron excitability and synaptic efficacy.
A transgenic vector was generated including the entire coding region of the FBJ osteosarcoma oncogene (Fos). The C-terminal of the Fos gene was fused to an enhanced green fluorescence protein (EGFP) followed by a polyadenylation signal. The donating investigator stated that the construct was microinjected into (DBA x C57BL/6) F1 fertilized oocytes, and mice from founder line 1-3 were bred to C57BL/6 mice (see SNP note below) for at least 17 generations. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 4 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | Fos/EGFP fusion protein is expressed in recently activated neurons. |
Allele Name | transgene insertion 1-3, Alison Barth |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | fosGFP |
Gene Symbol and Name | Tg(Fos/EGFP)1-3Brth, transgene insertion 1-3, Alison Barth |
Gene Synonym(s) | |
Promoter | Fos, FBJ osteosarcoma oncogene, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | Fos/EGFP fusion protein is expressed in recently activated neurons. |
Strain of Origin | (DBA x C57BL/6)F1 |
Chromosome | UN |
Molecular Note | This transgene contains a fusion of the c-fos promoter and the c-fos coding region, including exonsand introns, to the coding region for enhanced green fluorescent protein (EGFP) creating a fosGFP C-terminus fusion protein. The gene for EGFP was fused in frame to the last exon, and the poly(A) tail is derived from the IRES (phosphorylated-internal ribosomal entry site)-EGFP. Four lines were established (lines 1-3, 4-1, 5-1, and 6-1) with slightly different levels of expression in the central nervous system. |
Mutations Made By | Alison Barth, Carnegie Mellon University |
When maintaining a live colony, hemizygous mice may be bred to wildtype mice (non-carriers) from the colony or C57BL/6J inbred mice (Stock No. 000664).
When using the fosGFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #014135 in your Materials and Methods section.
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Fos/EGFP)1-3Brth |
Frozen Mouse Embryo | B6.Cg-Tg(Fos/EGFP)1-3Brth/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Fos/EGFP)1-3Brth/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Fos/EGFP)1-3Brth/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Fos/EGFP)1-3Brth/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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