This strain harbors mutations in the Maob and Maob genes which abolish gene function. These mice exhibit increased reactivity to stress,increased aggression and may be useful for studying MAOA and MAOB-related behaviors and disorders.
Jean Shih, University of Southern California
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Maob | monoamine oxidase B |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Spontaneous | Maoa | monoamine oxidase A |
This strain carries two mutant alleles, a knockout of the X-linked monoamine oxidase B (Maob) gene and a spontaneous mutation designated MaoaK284stop in the monoamine oxidase A (Maoa) gene. The two mutations lie 24kb apart. MAO A/B deficient mice are viable, fertile, and normal in size. Maoa and Maob are mitochondrial enzymes which oxidize neurotransmitters and dietary amines. These mutants lack both MAOA and MAOB activity in the brain and liver resulting in increased levels of phenylethylamine, serotonin, dopamine, and norepinephrine. These mice exhibit increased reactivity to stress and increased aggression. These mice may be useful for studying MAOA and MAOB-related behaviors and disorders.
A targeting vector was designed to replace exon 6 of the X-linked monoamine oxidase B (Maob) gene with a neomycin resistance (neo) cassette. The construct was electroporated into either 129S/SvEv-derived CCE embryonic stem (ES) cells or 129S4/SvJae-derived CCE ES cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to 129/Sv females. The male offspring were backcrossed at least 25 generations to 129/Sv females resulting in Maob deficient mice (Stock No. 014133). A mutation, designated MaoaK284stop, arose spontaneously in exon 8 of the X-linked monoamine oxidase A (Maoa) gene of one male animal. This mutation was caused by a transversion at nucleotide 863, resulting in a premature stop codon at residue 284. This mutant male was backcrossed to a 129/Sv female, and the resulting female offspring were bred to 129/Sv males. Half of the resulting male offspring carried both mutations. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. 4 of the 27 markers throughout the genome suggested a C57BL/6 genetic contribution from an unknown source.
Allele Name | targeted mutation 1, Jean C Shih |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | MAOB KO |
Gene Symbol and Name | Maob, monoamine oxidase B |
Gene Synonym(s) | |
Strain of Origin | 129S/Sv |
Chromosome | X |
General Note | ES cell line = CCE (129S/SvEv-Gpi1c) or J1 (129S4/SvJae). |
Molecular Note | The gene was disrupted by insertion of a neo cassette into exon 6 via homologous recombination resulting in a premature stop codon and protein truncation. Enzyme assays of brain and liver extracts from mutant mice demonstrated background levels of enzyme activity. Western blot and immunohistochemical analyses failed to detect protein product. |
Mutations Made By | Jean Shih, University of Southern California |
Allele Name | K284stop |
---|---|
Allele Type | Spontaneous |
Allele Synonym(s) | MAO A KO; MaoaA863T |
Gene Symbol and Name | Maoa, monoamine oxidase A |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | X |
Molecular Note | An A-to-T at coding nucleotide 850 in exon 8 created a stop codon at lysine codon 284 (p.K284*). |
Mutations Made By | Jean Shih, University of Southern California |
When maintaining a live colony, double homozygous females may be bred to double hemizygous males.
When using the B6;129S-MaoaK284stop Maobtm1Shih/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #014134 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
X linked -Heterozygous females and Non carrier males for Maoa<K284stop>, Heterozygous females and Non carrier males for Maob<tm1Shih>, 1 pair minimum |
Frozen Mouse Embryo | B6;129S-Maoa<K284stop> Maob<tm1Shih>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S-Maoa<K284stop> Maob<tm1Shih>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S-Maoa<K284stop> Maob<tm1Shih>/J | $3373.50 |
Frozen Mouse Embryo | B6;129S-Maoa<K284stop> Maob<tm1Shih>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.