Overview

Also Known As: B6N.Sox2-Cre , B6NJ.Sox2-Cre , B6N.Sox2Cre , B6NJ.Sox2Cre

These Sox2Cre transgenic mice express Cre recombinase under the control of the mouse Sox2 promoter, and may be useful for generating epiblast-derived specific conditional mutations.

Donating Investigator

IMR Colony, The Jackson Laboratory

Genetic overview

Genetic Background	Generation
	N17F1 (2019-07-25 00:00:00)

Edil3^{Tg(Sox2-cre)1Amc}

Allele Type
Transgenic (Recombinase-expressing, Null/Knockout)

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Research Applications

Research Tools

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Base Price

Starting at:

$278.00 Domestic price for female

356.51 Domestic price for breeder pair

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Details

Detailed Description

These transgenic mice express Cre recombinase under the control of the mouse SRY-box containing gene 2 promoter. The transgene integrated into an intron of Edil3 (EGF-like repeats and discoidin I-like domains 3). The insertion results in a functional knock-out of Edil3 in homozygous mice. Founder line 1 has a copy number of 2-5. Mice hemizygous for the Sox2Cre transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these transgenic mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in Cre-expressing tissues in the offspring. Specifically, Cre recombinase activity is detected in the epiblast cells at embryonic day 6.5, with little or no activity in other cells at gastrulation. Some activity is also detected in extra embryonic derivatives of the epiblast, the yolk sac mesoderm and amnion. No Cre recombinase activity is detected in primitive endoderm derived tissues, visceral endoderm. The phenotype of homozygous mice has not been characterized to date (April 2011). These Sox2Cre transgenic may be useful for generating epiblast-derived specific conditional mutations. Transgene expression is active in the female germline. Offspring arising from a hemizygous transgenic female will exhibit Cre recombinase activity, regardless of genotype. This maternal inheritance effect, due to female germline expression of the transgene, can provide a rapid and efficient breeding mechanism for generating null animals.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

The Sox2Cre transgene was designed with 12.5 kb of upstream regulatory sequence from the mouse Sox2 locus (SRY-box containing gene 2), a chicken β-actin intron, a Cre recombinase gene, and a rabbit β-globin poly(A) sequence. This transgene was introduced into B6CBAF1 donor eggs. The resulting founder animals were initially crossed to C57BL/6 mice, and then crossed to outbred Swiss Webster mice. The transgene integrated into an intron of Edil3 (EGF-like repeats and discoidin I-like domains 3) on chromosome 13. Founder line 1 has a copy number of 2-5. The donating investigator reported that the mice were backcrossed to C57BL/6 for 11 generations before being sent to The Jackson Laboratory Repository (as Stock No. 008454). There, some mice were subsequently backcrossed to C57BL/6NJ inbred mice (Stock No. 005304) for several generations using a marker-assisted, speed congenic approach to generate this C57BL/6N-congenic strain as Stock No. 014094.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	epiblast cells at embryonic day 6.5, with little or no activity in other cells at gastrulation; some activity is also detected in extra embryonic derivatives of the epi-blast, the yolk sac mesoderm and amnion; no activity is detected in primitive endoderm derived tissues, visceral endoderm

Control Suggestions

Noncarrier
005304 C57BL/6NJ

Additional Information

Considerations for Choosing Controls

Selected References

Hayashi S; Lewis P; Pevny L; McMahon AP. 2002. Efficient gene modulation in mouse epiblast using a Sox2Cre transgenic mouse strain. Mech Dev 119 Suppl 1:S97-S101PubMed: 14516668 MGI: J:83040

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Genetics

Edil3^{Tg(Sox2-cre)1Amc}

Allele Symbol: Edil3^{Tg(Sox2-cre)1Amc}

Allele Name	transgene insertion 1, Andrew P McMahon
Allele Type	Transgenic (Recombinase-expressing, Null/Knockout)
Allele Synonym(s)	transgene insertion 1, Andrew P McMahon; Edil3^{Tg(Sox2-cre)1Amc}
Gene Symbol and Name	Edil3, EGF-like repeats and discoidin I-like domains 3
Gene Synonym(s)	Del-1; developmental endothelial locus-1; DEL1; Del1
Promoter	Sox2, SRY (sex determining region Y)-box 2, mouse, laboratory
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	epiblast cells at embryonic day 6.5, with little or no activity in other cells at gastrulation; some activity is also detected in extra embryonic derivatives of the epi-blast, the yolk sac mesoderm and amnion; no activity is detected in primitive endoderm derived tissues, visceral endoderm
Strain of Origin	(C57BL/6 x CBA)F1
Chromosome	13
General Note	Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities.
Molecular Note	This transgene expresses Cre recombinase under the control of a mouse Sox2 promoter contained in 12.5 kb of upstream DNA. Expression of this transgene is sex-dependent, with increased efficiency when passed through the female germline. When the transgene is passed through the male germline, cre activity is observed in epiblast cells as early as E6.5 of only those embryos that inherit the transgene and no expression is detected in extraembryonic tissue. When the transgene is passed through the female germline, cre activity is observed throughout the embryo (including the yolk sac) in all early embryos regardless of whether or not they inherit the transgene. Line 1 inserted into an intron of the gene at 89311726-89311727 (Build GRCm38/mm10) resulting in a functional knockout of the gene. Founder line 1 has a copy number of 2-5.
Mutations Made By	Andrew McMahon, University of Southern California

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Developmental Biology Research
  - Cre-lox System
- Genetics Research
  - Mutagenesis and Transgenesis
  - Mutagenesis and Transgenesis: Cre-lox System
- Cre-lox System
  - Cre Recombinase Expression
  - Cre Recombinase Expression: Germline/Embryonic Expression

Mammalian Phenotype Terms by Genotype

References

Hayashi S; Lewis P; Pevny L; McMahon AP. 2002. Efficient gene modulation in mouse epiblast using a Sox2Cre transgenic mouse strain. Mech Dev 119 Suppl 1:S97-S101PubMed: 14516668 MGI: J:83040

Additional - Edil3^{Tg(Sox2-cre)1Amc} related

Technical Support

Contact Technical Support

Genotyping Protocols
- MELT: Edil3^{Tg(Sox2-cre)1Amc}
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

Transgenic mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for many generations to establish this congenic strain. When maintaining the live congenic colony, hemizygous mice may be bred with wildtype (noncarrier) siblings or to C57BL/6NJ inbred mice (Stock No. 005304). The phenotype of homozygous mice has not been characterized to date (April 2011). Of note, transgene expression is active in the female germline. Offspring arising from a hemizygous transgenic female will exhibit Cre recombinase activity, regardless of phenotype.
- Additional Breeding and Husbandry Support
Mating System
- Hemizygote x Noncarrier
- Noncarrier x Hemizygote
Citation
When using the B6N.Sox2-Cre , B6NJ.Sox2-Cre , B6N.Sox2Cre , B6NJ.Sox2Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #014094 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX9 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service	Genotype	Price
	Hemizygous or non carrier for Tg(Sox2-cre)1Amc

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Frozen Mouse Embryo

$2,595.00 per straw or vial

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: B6N.Sox2-Cre , B6NJ.Sox2-Cre , B6N.Sox2Cre , B6NJ.Sox2Cre

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Edil3Tg(Sox2-cre)1Amc

Allele Symbol: Edil3Tg(Sox2-cre)1Amc

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Edil3Tg(Sox2-cre)1Amc related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Edil3^{Tg(Sox2-cre)1Amc}

Allele Symbol: Edil3^{Tg(Sox2-cre)1Amc}

Additional - Edil3^{Tg(Sox2-cre)1Amc} related