Expression of Cacnb2 (calcium channel, voltage-dependent, beta 2 subunit) is regulated by a tetracycline operator (tetO). This strain may be useful in studies of cardiomyopathy.
Jeffery D. Molkentin, Cincinnati Children's Hospital
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inducible, Inserted expressed sequence) |
Expression of Cacnb2 (calcium channel, voltage-dependent, beta 2 subunit) is regulated by a tetracycline operator (tetO); also called tetracycline-responsive element (TRE, TetRE) or tet-operator) in this transgenic strain. When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of CACNB2 protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. When crossed with a Myh6-tTA strain (e.g. Stock No. 003170), double transgenic mice show increased Ca2+ influx through cardiac L-type Ca2+ channels resulting in Ca2+ overload, myocyte disorganization, interstitial fibrosis, and cell death. Compound mutant animals show a 3.1-fold increase in CACNB2 expression. This strain may be useful in studies of cardiomyopathy.
A transgenic vector carrying rat Cacnb2a cDNA under the control of a tetO promoter was microinjected into FVB fertilized oocytes. The strain was maintained on an FVB background by the donating laboratory. This is the "low-expression" line.
Expressed Gene | Cacnb2, calcium voltage-gated channel auxiliary subunit beta 2, rat |
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Site of Expression |
Allele Name | transgene insertion 2, Jeffery D Molkentin |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(tetO-Cacnb2)2Jmol, transgene insertion 2, Jeffery D Molkentin |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | Cacnb2, calcium voltage-gated channel auxiliary subunit beta 2, rat |
Strain of Origin | FVB/N |
Chromosome | UN |
Molecular Note | The tetO promoter drives expression of a rat Cacnb2a cDNA. Line 2 is a low expressor. |
Mutations Made By | Jeffery Molkentin, Cincinnati Children's Hospital |
When maintained as a live colony, hemizygotes may be bred.
When using the FVB-Tg(tetO-Cacnb2)2Jmol/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013779 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(tetO-Cacnb2)2Jmol |
Frozen Mouse Embryo | FVB-Tg(tetO-Cacnb2)2Jmol/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(tetO-Cacnb2)2Jmol/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(tetO-Cacnb2)2Jmol/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(tetO-Cacnb2)2Jmol/J Frozen Embryo | $3373.50 |
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