These Cacna1h knock-out mice have constitutively constricted coronary arterioles and focal myocardial fibrosis. This strain may be useful in studies of coronary artery function and disease.
Kevin Campbell, University of Iowa
Genetic Background | Generation |
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N6+pN1F2
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Cacna1h | calcium channel, voltage-dependent, T type, alpha 1H subunit |
Homozygous Cacna1h (calcium channel, voltage-dependent, T type, alpha 1H subunit; also called α1 3.2) targeted mutant mice have constitutively constricted coronary arterioles and focal myocardial fibrosis. Isolated arteries show normal contractile responses but reduced relaxation in response to acetylcholine and nitroprusside. Diffuse areas of cardiac fribrosis are observed in ventricular walls from 10-week old mice, but not those of wildtype mice. At 1 year of age, their hearts have a more severe cardiac pathology, including larger areas of fibrosis, necrosis and lymphocyte infiltration. Homozygotes are smaller than littermate controls. Heterozygotes do not show any abnormalities. Northern and/or Western blot analysis of brain, testis and dorsal root ganglion tissues confirms the loss of RNA and protein in mice. This strain may be useful in studies of coronary artery function and disease.
Exon 6, encoding amino acid residues 216-267, was replaced with a loxP-flanked neomycin resistance cassette in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. The donating investigator reports that mutant mice were backcrossed to C57BL/6 mice for six generations prior to sending to The Jackson Laboratory Repository (see SNP notes below). Upon arrival, mutant mice were bred to C57BL/6J inbred mice (Stock No. 000664) for one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with markers covering all 19 chromosomes and the X chromosome, was performed on the rederived living colony at The Jackson Laboratory Repository. This revealed markers on Chromosome 4 and 6, and two markers on chromosome 8 that were not fixed for C57BL/6 allele-type (e.g.: still segregating for 129X1/129S1 allele-type markers). These data suggests an incomplete backcross.
Allele Name | targeted mutation 1, Kevin P Campbell |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | alpha13.2-; Cav3.2- |
Gene Symbol and Name | Cacna1h, calcium channel, voltage-dependent, T type, alpha 1H subunit |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 17 |
Molecular Note | Exon 6, which encodes residues 216 through 267, was replaced with a floxed neo cassette inserted by homologous recombination. Transcript was undetected by Northern blot analysis of RNA obtained from homozygous mutant testes. Similarly, Western blot analysis of extracts from homozygous mutant brain tissue indicated an absence of normal protein. |
Mutations Made By | Kevin Campbell, University of Iowa |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the α13.2- mouse strain in a publication, please cite the originating article(s) and include JAX stock #013770 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Cacna1h<tm1Kcam> |
Frozen Mouse Embryo | B6;129-Cacna1h<tm1Kcam>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Cacna1h<tm1Kcam>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129-Cacna1h<tm1Kcam>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129-Cacna1h<tm1Kcam>/J Frozen Embryo | $3373.50 |
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