These knockout mice lack exons 1-2 of the Batf gene, abolishing gene function. This strain may be useful for studying TH17 cells differentiation associated with Multiple Sclerosis and Experimental Autoimmune Encephalomyelitis.
Dr. Kenneth Murphy, Washington University School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Batf | basic leucine zipper transcription factor, ATF-like |
These Batf-/- mutant mice lack exons 1-2 of the basic leucine zipper transcription factor(Batf), ATF-like gene, abolishing gene function. Mice that are homozygous for this allele are viable and fertile. Batf is highly expressed in T helper (TH) 1, TH2 and TH17 cells. Batf is required for the differentiation of interleukin (IL)-17-producing TH17 cells which are CD4+ T cells that coordinate inflammatory responses in host defense. These mice exhibit normal TH1 and TH2 differentiation, but show a defect in TH17 differentiation with a reduction in IL-17 production, but maintain normal levels of IL-2, interferon (IFN)-γ, and IL-10 under TH17 conditions. Since TH17 cells are the major pathogenic population in experimental autoimmune encephalomyelitis (EAE), these Batf-/- mice are completely resistant EAE even when immunized with myelin oligodendrocyte glycoprotein peptide 35-55 (MOG35-55), a peptide known to cause demyelination associated with multiple sclerosis (MS) and EAE.
A targeting vector was designed to replace exons 1-2 of the basic leucine zipper transcription factor, ATF-like (Batf) gene with a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into 129S6/SvEvTac-derived EDJ22 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre expressing Adenovirus to delete the neo cassette. Resulting ES cells were injected into blastocysts and resulting chimeric males were bred with 129SvEv inbred females to establish a colony of Batf-/- mice. The donating investigator stated that these mice were then backcrossed to C57BL/6 mice (see SNP note below) for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Allele Name | targeted mutation 1.1, Kenneth M Murphy |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Batf, basic leucine zipper transcription factor, ATF-like |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 12 |
Molecular Note | Exons 1 and 2 were replaced with a floxed neomycin cassette. The neomycin cassette was subsequently removed by transient expression of cre recombinase. No protein product was detected by immunoblot analysis of homozygote splenocyte or Th17 cells. |
Mutations Made By | Dr. Kenneth Murphy, Washington University School of Medicine |
When maintaining a live colony, homozygous mice may be bred together.
When using the Batf- mouse strain in a publication, please cite the originating article(s) and include JAX stock #013758 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Batf<tm1.1Kmm> |
Frozen Mouse Embryo | B6.129S-Batf<tm1.1Kmm>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S-Batf<tm1.1Kmm>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S-Batf<tm1.1Kmm>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S-Batf<tm1.1Kmm>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.