STOCK Tg(CAG-KikGR)33Hadj/J

Stock number: 013753
Research areas: Research Tools

Description

CAG::KikGR³³ transgenic mice express a Kikume Green-Red photoconvertible fluorescent protein under the control of a CMV enhancer/chicken beta-actin promoter promoter. These mice are useful as reporters for studying individual cell behaviors, population dynamics and cell fate in embryos and adult mice.

Detailed description

CAG::KikGR³³ transgenic mice express a Kikume Green-Red (KikGR) photoconvertible fluorescent protein under the control of a CMV enhancer/chicken beta-actin promoter (CAGGS) promoter. Mice homozygous for the transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. KikGR, engineered from Favia favus coral, changes color from green to red upon activation in embryos, adult mice, and embryonic stem (ES) cells. At basal state, green fluorescence is seen in all cells. A single cell or group of cells at basal state, exposed to 405 nm wavelength light, undergo photo conversion and fluoresce red. Since KikGR is developmentally neutral and non-toxic, the movement of these fluorescent cells, and their progeny, can be imaged during embryonic development. Mice from founder line 33 exhibits widespread expression of KikGR, while mice from founder line 75 (Stock No. 013754) exhibited widespread KikGR expression in a more mosaic pattern, allowing the analysis of cell morphology and cell behavior dynamics. These mice are useful as reporters for studying individual cell behaviors, population dynamics and cell fate in embryos and adult mice.

Development

The CAG::KikGR transgene was designed with a CMV enhancer/chicken beta-actin promoter (CAGGS) promoter driving widespread expression of a Kikume Green-Red (KikGR) photoconvertible fluorescent protein (engineered from Favia favus), followed by a rabbit beta-globin polyadenylation site. The transgene was microinjected into fertilized C57BL/6 oocytes, and mice from founder line 33 were bred to outbred ICR mice to establish a colony of CAG::KikGR³³ mice. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.

Allele

Symbol: Tg(CAG-KikGR)33Hadj
Name: transgene insertion 33, Anna-Katerina Hadjantonakis
MGI accession ID: MGI:4836188
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(CAG-KikGR)33Hadj
Marker name: transgene insertion 33, Anna-Katerina Hadjantonakis
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: GFP,Green Fluorescent Protein,; RFP,Red Fluorescent Protein,coral
Site of expression: Mice from founder line 33 exhibit widespread expression of Kikume Green-Red (KikGR) photoconvertible fluorescent protein.
Molecular note: The CAG::KikGR transgene was designed with a CMV enhancer/chicken beta-actin promoter (CAGGS) promoter driving widespread expression of a Kikume Green-Red (KikGR) photoconvertible fluorescent protein (engineered from Favia favus), followed by a rabbit beta-globin polyadenylation site. Lines 33 and 75 were established. Mice from founder line 33 exhibit widespread expression of KikGR, while mice from founder line 75 exhibit widespread KikGR expression in a mosaic pattern allowing for analyzing cell morphology and determining cell behavior dynamics.