CAG::KikGR33 transgenic mice express a Kikume Green-Red photoconvertible fluorescent protein under the control of a CMV enhancer/chicken beta-actin promoter promoter. These mice are useful as reporters for studying individual cell behaviors, population dynamics and cell fate in embryos and adult mice.
Anna-Katerina Hadjantonakis, Memorial Sloan-Kettering Cancer Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
CAG::KikGR33 transgenic mice express a Kikume Green-Red (KikGR) photoconvertible fluorescent protein under the control of a CMV enhancer/chicken beta-actin promoter (CAGGS) promoter. Mice homozygous for the transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. KikGR, engineered from Favia favus coral, changes color from green to red upon activation in embryos, adult mice, and embryonic stem (ES) cells. At basal state, green fluorescence is seen in all cells. A single cell or group of cells at basal state, exposed to 405 nm wavelength light, undergo photo conversion and fluoresce red. Since KikGR is developmentally neutral and non-toxic, the movement of these fluorescent cells, and their progeny, can be imaged during embryonic development. Mice from founder line 33 exhibits widespread expression of KikGR, while mice from founder line 75 (Stock No. 013754) exhibits widespread KikGR expression in a more mosaic pattern, allowing the analysis of cell morphology and cell behavior dynamics. These mice are useful as reporters for studying individual cell behaviors, population dynamics and cell fate in embryos and adult mice.
The CAG::KikGR transgene was designed with a CMV enhancer/chicken beta-actin promoter (CAGGS) promoter driving widespread expression of a Kikume Green-Red (KikGR) photoconvertible fluorescent protein (engineered from Favia favus), followed by a rabbit beta-globin polyadenylation site. The transgene was microinjected into fertilized C57BL/6 oocytes, and mice from founder line 33 were bred to outbred ICR mice to establish a colony of CAG::KikGR33 mice. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | RFP, Red Fluorescent Protein, coral |
Site of Expression | Mice from founder line 33 exhibit widespread expression of Kikume Green-Red (KikGR) photoconvertible fluorescent protein. |
Allele Name | transgene insertion 33, Anna-Katerina Hadjantonakis |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | CAG::KikGR33 |
Gene Symbol and Name | Tg(CAG-KikGR)33Hadj, transgene insertion 33, Anna-Katerina Hadjantonakis |
Gene Synonym(s) | |
Promoter | CMV, cytomegalovirus, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | RFP, Red Fluorescent Protein, coral |
Site of Expression | Mice from founder line 33 exhibit widespread expression of Kikume Green-Red (KikGR) photoconvertible fluorescent protein. |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | The CAG::KikGR transgene was designed with a CMV enhancer/chicken beta-actin promoter (CAGGS) promoter driving widespread expression of a Kikume Green-Red (KikGR) photoconvertible fluorescent protein (engineered from Favia favus), followed by a rabbit beta-globin polyadenylation site. Lines 33 and 75 were established. Mice from founder line 33 exhibit widespread expression of KikGR, while mice from founder line 75 exhibit widespread KikGR expression in a mosaic pattern allowing for analyzing cell morphology and determining cell behavior dynamics. |
Mutations Made By | Sonja Nowotschin, Memorial Sloan-Kettering Cancer Center |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or C57BL/6J (Stock No. 000664).
When using the STOCK Tg(CAG-KikGR)33Hadj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013753 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(CAG-KikGR)33Hadj |
Frozen Mouse Embryo | STOCK Tg(CAG-KikGR)33Hadj/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(CAG-KikGR)33Hadj/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(CAG-KikGR)33Hadj/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(CAG-KikGR)33Hadj/J Frozen Embryo | $3373.50 |
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