TCF/Lef:H2B-GFP transgenic mice express an H2B-EGFP fusion protein under the control of six copies of a T cell specific transcription factor/lymphoid enhancer-binding factor 1 response element and a heat shock protein 1B minimal promoter. These mice may be useful for visualizing Wnt/β-catenin signaling activity during early embryogenesis and allow the tracking of individual Wnt-expressing cells.
Anna-Katerina Hadjantonakis, Memorial Sloan-Kettering Cancer Center
Genetic Background | Generation |
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F?+N2F20
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Allele Type |
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Transgenic (Reporter) |
Starting at:
$255.00 Domestic price for female 4-week |
333.51 Domestic price for breeder pair |
TCF/Lef:H2B-GFP transgenic mice express an H2B-EGFP fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Green Fluorescent Protein gene) under the control of six copies of a T cell specific transcription factor/lymphoid enhancer-binding factor 1 (TCF/Lef1) response element and a heat shock protein 1B (Hspa1b) minimal promoter. Mice homozygous for the transgene are viable, fertile, and normal in size. Wnt (wingless-related MMTV) family members are required for triggering embryonic axis formation and for proper development. Wnt signaling results in phosphorylation and nuclear localization of β-catenin, a transcriptional co-activator protein, which, together with the TCF/Lef family of transcription factors, induces the transcription of downstream genes. TCF/Lef:H2B-GFP transgenic mice contain 6 copies of nuclear localized TCF/Lef1 DNA binding sites, which provides increased sensitivity to Wnt/&beta-catenin signaling and drives single-cell resolution of GFP expression at sites of active signaling. Specifically, GFP expression is evident in cells of the extraembryonic visceral endoderm, the primitive streak, the mature node, presomitic mesoderm and newly formed somites, posterior neural plate, cranial neural crest, face, and branchial arches. By E12.5 fluorescence is also seen in the kidney, gonads, and some neuronal subsets in the brain.
The TCF/Lef:H2B-GFP transgene was designed with six copies of a T cell specific transcription factor/lymphoid enhancer-binding factor 1 (TCF/Lef1) DNA binding site and a heat shock protein 1B (Hspa1b) minimal promoter driving expression of an H2B-EGFP fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Green Fluorescent Protein gene), followed by a simian virus 40 (SV40) polyadenylation site. The transgene was microinjected into fertilized C57BL/6 X 129 oocytes, and founder mice were bred to outbred ICR mice to establish a colony. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.
Expressed Gene | H2BC3, H2B clustered histone 3, human |
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Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP expression is evident in cells of the extraembryonic visceral endoderm, the primitive streak, the mature node, presomitic mesoderm and newly formed somites, posterior neural plate, cranial neural crest, face, and branchial arches. By E12.5 fluorescence is also seen in the kidney, gonads, and some neuronal subsets in the brain. |
Allele Name | transgene insertion 61, Anna-Katerina Hadjantonakis |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | TCF/Lef:H2B/GFP |
Gene Symbol and Name | Tg(TCF/Lef1-HIST1H2BB/EGFP)61Hadj, transgene insertion 61, Anna-Katerina Hadjantonakis |
Gene Synonym(s) | |
Promoter | Hspa1b, heat shock protein 1B, mouse, laboratory |
Expressed Gene | H2BC3, H2B clustered histone 3, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP expression is evident in cells of the extraembryonic visceral endoderm, the primitive streak, the mature node, presomitic mesoderm and newly formed somites, posterior neural plate, cranial neural crest, face, and branchial arches. By E12.5 fluorescence is also seen in the kidney, gonads, and some neuronal subsets in the brain. |
Strain of Origin | C57BL/6 x 129 |
Chromosome | UN |
Molecular Note | The TCF/Lef:H2B-GFP transgene was designed with six copies of a T cell specific transcription factor/lymphoid enhancer-binding factor 1 (TCF/Lef1) DNA binding site and a heat shock protein 1B (Hspa1b) minimal promoter driving expression of an H2B-EGFP fusion protein (coding sequence for the human HIST1H2BB gene [histone 1 H2bb] followed C-terminally by Enhanced Green Fluorescent Protein gene), followed by a simian virus 40 (SV40) polyadenylation site. |
Mutations Made By | Anna-Katerina Hadjantonakis, Memorial Sloan-Kettering Cancer Center |
When maintaining a live colony, hemizygous mice may be bred to wildtype mice from the colony.
When using the TCF/Lef:H2B/GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #013752 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or non carrier for Tg(TCF/Lef1-HIST1H2BB/EGFP)61Hadj |
Frozen Mouse Embryo | STOCK Tg(TCF/Lef1-HIST1H2BB/EGFP)61Hadj/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(TCF/Lef1-HIST1H2BB/EGFP)61Hadj/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(TCF/Lef1-HIST1H2BB/EGFP)61Hadj/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(TCF/Lef1-HIST1H2BB/EGFP)61Hadj/J Frozen Embryo | $3373.50 |
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