These Slc9a4 (or Nhe4) knockout mice exhibit a reduction in gastric acid, reduced numbers of parietal cells, a loss of mature chief cells, increased numbers of mucous and undifferentiated cells, and an increase in the number of necrotic and apoptotic cells. These mice may be useful for studying gastric acid secretion, gastric epithelial cell differentiation, and development of secretory canalicular and tubulovesicular membranes.
Gary E Shull, University of Cincinnati
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Slc9a4 | solute carrier family 9 (sodium/hydrogen exchanger), member 4 |
Homozygotes: A targeting vector was designed to insert a neomycin resistance (neo) cassette into exon 2 of the solute carrier family 9 (sodium/hydrogen exchanger), member 4 (Slc9a4 or Nhe4) gene, abolishing gene function. Homozygous Nhe4-/- mice are viable, fertile, and normal in size. Nhe4 is expressed on the basolateral membrane of gastric parietal cells and zymogenic (chief) cells, and is required for normal levels of gastric acid secretion, secretory membrane development, and parietal cell and chief cell differentiation. These mutant mice display a reduction in gastric acid and their gastric mucosa contain reduced numbers of parietal cells, a loss of mature chief cells, increased numbers of mucous and undifferentiated cells, and an increase in the number of necrotic and apoptotic cells. In addition, the parietal cells of Nhe4-/- mice exhibit limited development of canalicular membranes and a virtual absence of tubulovesicles. These mice may be useful for studying gastric acid secretion, gastric epithelial cell differentiation, and development of secretory canalicular and tubulovesicular membranes.
Heterozygote: Heterozygous mice are viable, fertile, and normal in size. They exhibit normal growth and are phenotypically similar to wildtype mice.
A targeting vector was designed to insert a neomycin resistance (neo) cassette into exon 2 of the solute carrier family 9 (sodium/hydrogen exchanger), member 4 (Slc9a4 or Nhe4) gene. The construct was electroporated into 129S6/SvEvTac-derived embryonic stem (ES) cells and correctly targeted ES cells were injected into blastocysts. The resulting chimeric males were bred to Black Swiss females. Upon arrival at the The Jackson Laboratory, mice were bred to 129S1/SvImJ inbred mice for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Gary E Shull |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | NHE4- |
Gene Symbol and Name | Slc9a4, solute carrier family 9 (sodium/hydrogen exchanger), member 4 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 1 |
Molecular Note | A targeting construct was designed to insert a neomycin resistance into exon 2. Northern blot demonstrated a reduction in the size of the transcript in mutant stomach tissue. Sequence analysis showed that exon 2 and the neo gene had been excised and exon 1 was spliced to exon 3. |
Mutations Made By | Gary Shull, University of Cincinnati |
When maintaining a live colony, homozygous mice may be bred together.
When using the NHE4- mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #34266 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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