These lckpr-bcl-XL transgenic mice express a human BCL2L1 cDNA sequence under the control of a mouse Lck promoter. These mice may be used for studying development, maturation, and apoptosis of thymocytes.
Craig B. Thompson, Memorial Sloan Kettering Cancer Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
These lckpr-bcl-XL transgenic mice express a human B-cell leukemia/lymphoma 2 like, 1 (BCL2L1 or BCL-XL) cDNA sequence, under the control of a mouse lymphocyte protein tyrosine kinase proximal promoter (Lckpr). Mice that are heterozygous for this transgene are viable, fertile, and normal in size. Lckpr drives BCL-XL expression within all thymocyte subsets and protects thymocytes from a variety of apoptotic stimuli, including γ irradiation, glucocorticoids, and anti-CD3 treatment. This BCL-XL overexpression alters thymocyte maturation, resulting in an increase in CD3int/hi and a decrease in CD3lo thymocytes, along with an increase in intermediate CD4+8lo and CD4lo8+ thymocytes. They also exhibit an increase in single positive CD8 T cells in the spleen. Overexpression of BCL-XL causes down-regulation of B-cell leukemia/lymphoma 2 (BCL-2) and represses cell death observed in BCL-2 null mice, suggesting a redundant cell survival pathway. These mice may be used for studying development, maturation, and apoptosis of thymocytes.
The LCKprBCL2L1 transgene was designed with the human B-cell leukemia/lymphoma 2 like, 1 (BCL2L1) cDNA driven by a mouse lymphocyte protein tyrosine kinase (Lck) proximal promoter, followed by a human growth hormone (hGH) gene and a simian virus 40 (SV40) polyadenylation site. The transgene was microinjected into fertilized oocytes, and mice from founder line 12 were bred to C57BL/6J mice for at least 10 generations to generate a colony of lckpr-bcl-xL mice. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) to establish the colony.
Expressed Gene | BCL2L1, BCL2 like 1, human |
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Site of Expression |
Allele Name | transgene insertion 12, Stanley J Korsmeyer |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | Bcl-xL; Bcl-xLTg; Bcl-xL-Tg; lckpr-bcl-xL |
Gene Symbol and Name | Tg(LCKprBCL2L1)12Sjk, transgene insertion 12, Stanley J Korsmeyer |
Gene Synonym(s) | |
Promoter | Lck, lymphocyte protein tyrosine kinase, mouse, laboratory |
Expressed Gene | BCL2L1, BCL2 like 1, human |
Strain of Origin | Not Specified |
Chromosome | UN |
General Note | Transgenic lines 12 and 24, which expressed high amounts of the transgene, were established. Most of the analysis was performed using line 12 and confirmed with line 24. |
Molecular Note | The transgene consists of 0.75 kb of Lck proximal promoter sequences and 0.8 kb of human BCL2L1 cDNA. Introns, exons, and polyadenylation sequences from the human growth hormone gene consitutes the 3' untranslated region of the construct. The Lck promoter was used to drive transgene expression in lymphoid tissues. Transgene expression in the thymus and spleen was verified by Western blot analysis and flow cytometry. |
Mutations Made By | Dr. Stanley Korsmeyer, Dana-Farber Cancer Institute |
When maintaining a live colony, heterozygous mice may be bred to wildtype (non-carrier) mice from the colony or C57BL/6J inbred mice (Stock No. 000664).
When using the B6.Cg-Tg(LCKprBCL2L1)12Sjk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013738 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(LCKprBCL2L1)12Sjk |
Frozen Mouse Embryo | B6.Cg-Tg(LCKprBCL2L1)12Sjk/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(LCKprBCL2L1)12Sjk/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(LCKprBCL2L1)12Sjk/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(LCKprBCL2L1)12Sjk/J Frozen Embryo | $3373.50 |
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