behavior/neurological phenotype

• abnormal long term spatial reference memory
  • mutants are significantly slower at locating the hidden platform in the Morris water maze beyond day 3 of testing, but not at earlier times, indicating memory deficits
  (MGI Ref ID J:76361)

• abnormal spatial learning
  • subtle differences in performance of a probe trial, with mutants showing similar amounts of time spent in all quadrants while wild-type mutants spent more time in the training quadrant
  (MGI Ref ID J:76361)
  • in a two trial platform reversal test, the time required for mutants to locate the platform in the training and in the opposite quadrant were similar unlike in controls which find the platform in the training quadrant faster than in the opposite quadrant
  (MGI Ref ID J:76361)
  • Normal - however, intensive training can reverse the spatial learning deficit
  (MGI Ref ID J:76361)

• abnormal eye blink conditioning behavior
  • mutants exhibit impaired eye blink conditioning; after five training sessions, mutants show a conditioned response only about 50% of the time compared to the 80% seen in wild-type
  (MGI Ref ID J:76361)

nervous system phenotype

• abnormal acid-activated cation-mediated receptor currents
  • Normal - baseline synaptic transmission in the hippocampus is normal
  (MGI Ref ID J:76361)
  • transient acid-evoked cation currents are absent in hippocampal neurons, however currents activated by GABA, AMPA, and NMDA are normal
  (MGI Ref ID J:76361)

• abnormal excitatory postsynaptic potential
  • EPSP facilitation during high-frequency stimulation is impaired
  (MGI Ref ID J:76361)
  • Normal - however paired pulse facilitation is intact
  (MGI Ref ID J:76361)

• abnormal long term potentiation
  • Normal - however, LTP is restored following high-frequency stimulation in the presence of 10 uM phorbol 12-myristate 13-acetate (PMA) or in the presence of low Mg2+
  (MGI Ref ID J:76361)
  • hippocampal long-term potentiation is impaired
  (MGI Ref ID J:76361)