B6.129-Asic1^tm1Wsh/J

Stock number: 013733
Research areas: Neurobiology Research, Sensorineural Research

Description

These Asic1 knock-out mice exhibit alterations in conditioned and unconditioned fear behaviors, eye blink conditioning, long term potentiation, learned helplessness, seizure termination, retinal function, pain perception, and neuronal death in several neurodegnerative disorders.

Detailed description

Homozygous Asic1 (amiloride-sensitive cation channel 2, neuronal; also called ASIC1a) targeted mutant mice display alterations in conditioned and unconditioned fear behaviors, eye blink conditioning, long term potentiation, learned helplessness, seizure termination, retinal function, pain perception, and neuronal death in several neurodegnerative disorders. Transcripts and protein are lacking in central and peripheral nervous system tissue. This strain may be useful in studies of synaptic transmission, synaptic plasticity, depression, fear, anxiety, learning and memory.

Development

Exon 1 and approximately 400 bp of upstream sequence (encoding the first 121 amino acids of the protein) was replaced with a neomycin resistance cassette in (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. The deleted region includes the intracellular N terminus, the first transmembrane domain, and a portion of the extracellular domain. This strain was backcrossed to C57BL/6 for more than 10 generations by the donating laboratory.

Allele

Symbol: Asic1^tm1Wsh
Name: targeted mutation 1, Michael J Welsh
MGI accession ID: MGI:2179834
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Asic1
Marker name: acid-sensing (proton-gated) ion channel 1
Chromosome: 15
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Exon 1 of this gene was replaced with a PGK-neo cassette via homologous recombination resulting in the deletion of the first 121 amino acids, which includes the intracellular N terminus, the first transmembrane domain, and a portion of the extracellular domain. Immunoprecipitation and Western blot analysis failed to detect protein in extracts from brain and hippocampus of homozygous mutant animals.