These Asic1 knock-out mice exhibit alterations in conditioned and unconditioned fear behaviors, eye blink conditioning, long term potentiation, learned helplessness, seizure termination, retinal function, pain perception, and neuronal death in several neurodegnerative disorders.
Michael Welsh, University of Iowa
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Asic1 | acid-sensing (proton-gated) ion channel 1 |
Homozygous Asic1 (amiloride-sensitive cation channel 2, neuronal; also called ASIC1a) targeted mutant mice display alterations in conditioned and unconditioned fear behaviors, eye blink conditioning, long term potentiation, learned helplessness, seizure termination, retinal function, pain perception, and neuronal death in several neurodegnerative disorders. Transcripts and protein are lacking in central and peripheral nervous system tissue. This strain may be useful in studies of synaptic transmission, synaptic plasticity, depression, fear, anxiety, learning and memory.
Exon 1 and approximately 400 bp of upstream sequence (encoding the first 121 amino acids of the protein) was replaced with a neomycin resistance cassette in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. The deleted region includes the intracellular N terminus, the first transmembrane domain, and a portion of the extracellular domain. This strain was backcrossed to C57BL/6 for more than 10 generations by the donating laboratory.
Allele Name | targeted mutation 1, Michael J Welsh |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | ASIC-; ASIC1-; ASIC1a- |
Gene Symbol and Name | Asic1, acid-sensing (proton-gated) ion channel 1 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 15 |
Molecular Note | Exon 1 of this gene was replaced with a PGK-neo cassette via homologous recombination resulting in the deletion of the first 121 amino acids, which includes the intracellular N terminus, the first transmembrane domain, and a portion of the extracellular domain. Immunoprecipitation and Western blot analysis failed to detect protein in extracts from brain and hippocampus of homozygous mutant animals. |
Mutations Made By | Michael Welsh, University of Iowa |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6.129-Asic1tm1Wsh/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013733 in your Materials and Methods section.
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Service/Product | Description | Price |
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Heterozygous for Accn2<tm1Wsh> |
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