These mice express a human aminopeptidase puromycin sensitive (hPSA or NPEPPS) gene directed by its endogenous promoter/enhancer regions on the BAC transgene. These mice may be useful for studying PSA protection in neurodegenerative disorders.
Stanislav Karsten, LABioMed at Harbor-UCLA Medical Center
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence, Humanized sequence) |
These mice express a human aminopeptidase puromycin sensitive (hPSA or NPEPPS) gene directed by its endogenous promoter/enhancer regions on a BAC transgene. Mice hemizygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. PSA is a cytosolic aminopeptidase that hydrolyzes N-terminal amino acids. PSA is considered to have neuroprotective qualities due to its ability to degrade certain proteins associated with neurodegenerative diseases (TAU and polyglutamine expansion repeat-containing proteins). hPSA overexpression is seen in the cerebral cortex, spinal cord, cerebellum, hippocampus, and brain stem at a 2-3 fold greater level than endogenous PSA. hPSA activity is also increased in muscle, kidney, and liver. These mice may be useful for studying PSA protection in neurodegenerative disorders.
Bacterial artificial chromosome (BAC) library was used to obtain a BAC (#RP11-592D23) containing the entire human aminopeptidase puromycin sensitive (hPSA or NPEPPS) gene. This BAC was microinjected into fertilized FVB/NTac oocytes. hPSA mice were maintained by breeding transgenic mice with FVB/NTac inbred mice for at least 15 generations. Upon arrival at The Jackson Laboratory, mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish the colony.
Expressed Gene | NPEPPS, aminopeptidase puromycin sensitive, human |
---|---|
Site of Expression |
Allele Name | transgene insertion 1, Stanislav Karsten |
---|---|
Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | hPSA |
Gene Symbol and Name | Tg(NPEPPS)1Skar, transgene insertion 1, Stanislav Karsten |
Gene Synonym(s) | |
Promoter | NPEPPS, aminopeptidase puromycin sensitive, human |
Expressed Gene | NPEPPS, aminopeptidase puromycin sensitive, human |
Strain of Origin | FVB/NTac |
Chromosome | UN |
Molecular Note | Bacterial artificial chromosome (BAC) library was used to obtain a BAC (#RP11-592D23) containing the entire human aminopeptidase puromycin sensitive (hPSA or NPEPPS) gene. |
Mutations Made By | Stanislav Karsten, LABioMed at Harbor-UCLA Medical Center |
When maintaining a live colony, hemizygous carriers may be bred with wildtype (non-carrier) mice from the colony or FVB/NJ inbred mice (Stock No. 001800).
When using the FVB-Tg(NPEPPS)1Skar/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013732 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(NPEPPS)1Skar |
Frozen Mouse Embryo | FVB-Tg(NPEPPS)1Skar/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(NPEPPS)1Skar/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(NPEPPS)1Skar/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(NPEPPS)1Skar/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.