JAX
Mouse Strain Datasheet - 013731
STOCK Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle/J
Also Known As: R26R-Brainbow2.1, R26R-Confetti
R26R-Confetti mice are available on a congenic C57BL/6J background (Stock No. 017492) and that congenic version will replace this mixed background strain in the near future.
The R26R-Confetti conditional allele has a CAG promoter followed by a floxed-STOP cassette and the Brainbow 2.1 construct all targeted into the Gt(ROSA)26Sor locus. The R26R-Confetti allele functions as a stochastic multicolor Cre recombinase reporter of multiple fluorescent proteins from a single genomic locus. These R26R-Confetti mice allow a way to label and distinguish individual / adjacent cells with nuclear localized, membrane-targeted, or cytoplasmic fluorescent proteins in cre recombined cells.
Donating Investigator
Hans Clevers, Hubrecht Institute
Genetic overview
| Genetic Background | Generation |
|---|---|
|
F?+F17
|
Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle
| Allele Type | Gene Symbol | Gene Name |
|---|---|---|
| Targeted (Conditional ready (e.g. floxed), Reporter) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
Detailed Description
Mice homozygous for the R26R-Confetti conditional allele are viable and fertile, with a CAG promoter, loxP site, and STOP cassette preventing transcription of the downstream Brainbow 2.1 sequences. The Brainbow 2.1 region contains two loxP-flanked dimers, each uniquely positioned in head-to-tail tandem. One dimer has nuclear-localized green fluorescent protein (hrGFPII) and a reverse-oriented cytoplasmic yellow fluorescent protein (mYFP). The other dimer has cytoplasmic red fluorescent protein (tdimer2(12)) and a reverse-oriented membrane-tethered cyan fluorescent protein (mCerulean). The Brainbow2.1 region may be written as loxP-STOP-loxP-GFP-PFY-Pxol-loxP-RFP-PFC-Pxol to show the transcriptional direction of each part. When bred to mice that express Cre recombinase, the resulting offspring may have a recombination event that stochastically places one of the four fluorescent proteins into position directly downstream of the CAG promoter within the cre-expressing tissues. Because this CAG promoter-driven Brainbow 2.1 reporter construct was targeted for insertion into the Gt(ROSA)26Sor locus, fluorescent protein expression is determined by which tissues express Cre recombinase. The donating investigator reports that mice do not express any fluorescent cells prior to introduction of Cre recombinase. The donating investigator confirms fluorescent protein expression following exposure to cre can be detected by direct fluorescence (and presumably also via mRNA (in situ hybridization) and antibody staining (immunohistochemistry)).
Initial Cre recombination outcomes may recombine the loxP-flanked STOP cassette (green), invert the loxP-STOP-loxP-GFP-PFY-Pxol region (yellow), recombine the loxP-STOP-loxP-GFP-PFY-Pxol-loxP region (red), or invert the entire loxP-STOP-loxP-GFP-PFY-Pxol-loxP-RFP-PFC-Pxol region (blue). Other recombination outcomes may not remove the STOP cassette and result in no fluorescent reporter labeling in cre-expressing cells. In addition, sequential recombination outcomes may reduce the construct to a single invertible dimer segment that can continue to invert as long as Cre recombinase is present. The donating investigator also reports that weaker cre expression favors inverting the loxP-STOP-loxP-GFP-PFY-Pxol region rather than removal of the loxP-STOP-loxP region: this results in less green-fluorescing cells / more non-fluorescing cells than is expected if using a strong cre-expressing line.
Development
A targeting vector containing (from 5' to 3') a strong CAGG promoter (CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter), a loxP site, a PGK-Neor-pA cassette (serving as a transcriptional roadblock), and the Brainbow 2.1 construct (described in greater detail below). This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus via electroporation into 129P2/OlaHsd-derived IB10/E14IB10 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6 females to generate the R26R-Confetti colony. The resulting R26R-Confetti colony were bred with other mutant or cre-expressing mice, but these other mutations were bred away from the R26R-Confetti colony. The R26R-Confetti mice are reported to be on a genetic background equivalent to ~2-3 backcross generations to C57BL/6 prior to sending to The Jackson Laboratory Repository in 2010. Upon arrival the mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
The Brainbow 2.1 construct was designed by Drs. Jeff Lichtman and Joshua Sanes (Harvard University) with four fluorescent protein sequences uniquely positioned in a tandem fashion and delimited by loxP sites in opposite orientation. Specifically, this Brainbow 2.1 coding region is composed of two adjacent floxed head-to-tail tandem dimers. The first head-to-tail dimer contains a loxP site and humanized Renilla GFP (hrGFPII; with nuclear localization signal plus polyA sequence) in forward orientation, and a loxP site and monomeric EYFP (mYFPA206K plus polyA sequence) in reverse orientation. The second head-to-tail dimer contains a loxP site and tdimer2(12) RFP plus polyA sequence in forward orientation, and a loxP site and mCerulean CFP (with membrane tethering palmitoylation sequence plus polyA sequence) in reverse orientation. A single frt site is located at the 3' end of the Brainbow 2.1 construct. The entire construct may be written as loxP-STOP-loxP-GFP-PFY-Pxol-loxP-RFP-PFC-Pxol-frt to show transcriptional direction of each part.
The hrGFPII variant of GFP (from Stratagene vector phrGFPII-C) has amino acid substitutions designed to improve spectral properties and performance in mammalian systems. The monomeric EYFP (mYFPA206K) has an amino acid substitution replacing a hydrophobic region with a positively charged residue designed to prevent dimerization. The tdimer2(12) RFP is a non-oligomerizing DsRed variant with a 12 residue linker fusing two copies of the protein (tandem dimer). The monomeric Cerulean (mCerulean) is a variant of ECFP (ECFPS72A/Y145A/H148D/A206K) with amino acid substitutions designed to improve spectral properties and prevent dimerization.
Expression Data
| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish |
|---|---|
| Expressed Gene | GFP, Green Fluorescent Protein, |
| Expressed Gene | RFP, Red Fluorescent Protein, coral |
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
| Site of Expression | Cre recombination results in stochastic multicolor fluorescent proteins being expressed from a single genomic locus in cre-expressing tissues. |
Control Suggestions
Selected References
- Livet J; Weissman TA; Kang H; Draft RW; Lu J; Bennis RA; Sanes JR; Lichtman JW. 2007. Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature 450(7166):56-62. PubMed: 17972876MGI: J:125961
- Snippert HJ; van der Flier LG; Sato T; van Es JH; van den Born M; Kroon-Veenboer C; Barker N; Klein AM; van Rheenen J; Simons BD; Clevers H. 2010. Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells. Cell 143(1):134-44. PubMed: 20887898MGI: J:164644
Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle
Allele Symbol: Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle
| Allele Name | targeted mutation 1, Hans Clevers |
|---|---|
| Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter) |
| Allele Synonym(s) | R26R-Brainbow2.1; R26R-Confetti |
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
| Gene Synonym(s) | AV258896; Gtrgeo26; Gtrgeo26; Gtrosa26; Gtrosa26; R26; ROSA26; SETD5-AS1; Thumpd3as1; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26 |
| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish |
| Expressed Gene | GFP, Green Fluorescent Protein, |
| Expressed Gene | RFP, Red Fluorescent Protein, coral |
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
| Site of Expression | Cre recombination results in stochastic multicolor fluorescent proteins being expressed from a single genomic locus in cre-expressing tissues. |
| Strain of Origin | 129P2/OlaHsd |
| Chromosome | 6 |
| Molecular Note | A targeting vector containing (from 5' to 3') a strong CAGG promoter, a loxP site, a PGK-Neo-pA cassette (serving as a transcriptional roadblock), and the Brainbow 2.1 construct. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The Brainbow 2.1 construct was designed by Drs. Jeff Lichtman and Joshua Sanes (Harvard University) with four fluorescent protein sequences uniquely positioned in a tandem fashion and delimited by loxP sites in opposite orientation. Specifically, this Brainbow 2.1 coding region is composed of two adjacent floxed head-to-tail tandem dimers. The first head-to-tail dimer contains a loxP site and humanized Renilla GFP (hrGFPII; with nuclear localization signal plus polyA sequence) in forward orientation and a loxP site and monomeric EYFP (mYFPA206K plus polyA sequence) in reverse orientation. The second head-to-tail dimer contains a loxP site and tdimer2(12) RFP plus polyA sequence in forward orientation, and a loxP site and mCerulean CFP (with membrane tethering palmitoylation sequence plus polyA sequence) in reverse orientation. A single frt site is located at the 3' end of the Brainbow 2.1 construct. |
| Mutations Made By | Hans Clevers, Hubrecht Institute |
Disease Terms
Research Areas By Genotype
This mouse can be used to support research in many areas including:
- Neurobiology Research
- Cre-lox System
- loxP-flanked Sequences
- loxP-flanked Sequences: Test/Reporter
- Fluorescent protein expression in neural tissue
- Cre-lox System
- Research Tools
- Cardiovascular Research
- Cre-lox System
- Cre-lox System
- loxP-flanked Sequences
- loxP-flanked Sequences: Test/Reporter
- Developmental Biology Research
- Cre-lox System
- transplantation marker for embryonic and adult tissue
- Fluorescent Proteins
- Genetics Research
- Mutagenesis and Transgenesis
- Mutagenesis and Transgenesis: Cre-lox System
- Tissue/Cell Markers
- Tissue/Cell Markers: Cre-lox System
- Tissue/Cell Markers: cell marker for bone marrow transplantation
- Tissue/Cell Markers: spermatogonial transplantation marker
- Tissue/Cell Markers: transplantation marker for embryonic and adult tissue
- Immunology, Inflammation and Autoimmunity Research
- T cell deficiency, xenograft/transplant host
- Neurobiology Research
- cell marker
- Reproductive Biology Research
- Cre-lox System
- spermatogonial transplantation marker
- transplantation marker for embryonic and adult tissue
- Toxicology Research
- B and T cell deficiency, xenograft transplant host
- xenograft/transplant host
- Cardiovascular Research
Genotype: GFP related
- Research Tools
- Fluorescent Proteins
Genotype: YFP related
- Research Tools
- Fluorescent Proteins
Mammalian Phenotype Terms by Genotype
The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain
Genotype: Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle/Gt(ROSA)26Sor+
involves: 129P2/OlaHsd
no phenotypic analysis
- no phenotypic analysis
- (MGI Ref ID J:164644)
References
- Livet J; Weissman TA; Kang H; Draft RW; Lu J; Bennis RA; Sanes JR; Lichtman JW. 2007. Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature 450(7166):56-62. PubMed: 17972876MGI: J:125961
- Snippert HJ; van der Flier LG; Sato T; van Es JH; van den Born M; Kroon-Veenboer C; Barker N; Klein AM; van Rheenen J; Simons BD; Clevers H. 2010. Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells. Cell 143(1):134-44. PubMed: 20887898MGI: J:164644
Additional - Gt(ROSA)26Sortm1(CAG-Brainbow2.1)Cle related
- Aure MH; Konieczny SF; Ovitt CE. 2015. Salivary gland homeostasis is maintained through acinar cell self-duplication. Dev Cell 33(2):231-7. PubMed: 25843887MGI: J:239286
- Barkauskas CE; Cronce MJ; Rackley CR; Bowie EJ; Keene DR; Stripp BR; Randell SH; Noble PW; Hogan BL. 2013. Type 2 alveolar cells are stem cells in adult lung. J Clin Invest 123(7):3025-36. PubMed: 23921127MGI: J:201625
- Chen H; Matsumoto K; Brockway BL; Rackley CR; Liang J; Lee JH; Jiang D; Noble PW; Randell SH; Kim CF; Stripp BR. 2012. Airway epithelial progenitors are region specific and show differential responses to bleomycin-induced lung injury. Stem Cells 30(9):1948-60. PubMed: 22696116MGI: J:194655
- Cheung KJ; Padmanaban V; Silvestri V; Schipper K; Cohen JD; Fairchild AN; Gorin MA; Verdone JE; Pienta KJ; Bader JS; Ewald AJ. 2016. Polyclonal breast cancer metastases arise from collective dissemination of keratin 14-expressing tumor cell clusters. Proc Natl Acad Sci U S A 113(7):E854-63. PubMed: 26831077MGI: J:230438
- Choi YS; Zhang Y; Xu M; Yang Y; Ito M; Peng T; Cui Z; Nagy A; Hadjantonakis AK; Lang RA; Cotsarelis G; Andl T; Morrisey EE; Millar SE. 2013. Distinct Functions for Wnt/beta-Catenin in Hair Follicle Stem Cell Proliferation and Survival and Interfollicular Epidermal Homeostasis. Cell Stem Cell 13(6):720-33. PubMed: 24315444MGI: J:204142
- Decker RS; Um HB; Dyment NA; Cottingham N; Usami Y; Enomoto-Iwamoto M; Kronenberg MS; Maye P; Rowe DW; Koyama E; Pacifici M. 2017. Cell origin, volume and arrangement are drivers of articular cartilage formation, morphogenesis and response to injury in mouse limbs. Dev Biol 426(1):56-68. PubMed: 28438606MGI: J:243210
- Degn SE; van der Poel CE; Firl DJ; Ayoglu B; Al Qureshah FA; Bajic G; Mesin L; Reynaud CA; Weill JC; Utz PJ; Victora GD; Carroll MC. 2017. Clonal Evolution of Autoreactive Germinal Centers. Cell 170(5):913-926.e19. PubMed: 28841417MGI: J:247831
- Demitrack ES; Gifford GB; Keeley TM; Carulli AJ; VanDussen KL; Thomas D; Giordano TJ; Liu Z; Kopan R; Samuelson LC. 2015. Notch signaling regulates gastric antral LGR5 stem cell function. EMBO J 34(20):2522-36. PubMed: 26271103MGI: J:226632
- Desai TJ; Brownfield DG; Krasnow MA. 2014. Alveolar progenitor and stem cells in lung development, renewal and cancer. Nature 507(7491):190-4. PubMed: 24499815MGI: J:207468
- Dyachuk V; Furlan A; Shahidi MK; Giovenco M; Kaukua N; Konstantinidou C; Pachnis V; Memic F; Marklund U; Muller T; Birchmeier C; Fried K; Ernfors P; Adameyko I. 2014. Neurodevelopment. Parasympathetic neurons originate from nerve-associated peripheral glial progenitors. Science 345(6192):82-7. PubMed: 24925909MGI: J:211702
- Dyment NA; Breidenbach AP; Schwartz AG; Russell RP; Aschbacher-Smith L; Liu H; Hagiwara Y; Jiang R; Thomopoulos S; Butler DL; Rowe DW. 2015. Gdf5 progenitors give rise to fibrocartilage cells that mineralize via hedgehog signaling to form the zonal enthesis. Dev Biol 405(1):96-107. PubMed: 26141957MGI: J:225829
- Feil S; Fehrenbacher B; Lukowski R; Essmann F; Schulze-Osthoff K; Schaller M; Feil R. 2014. Transdifferentiation of vascular smooth muscle cells to macrophage-like cells during atherogenesis. Circ Res 115(7):662-7. PubMed: 25070003MGI: J:234898
- Fioret BA; Heimfeld JD; Paik DT; Hatzopoulos AK. 2014. Endothelial Cells Contribute to Generation of Adult Ventricular Myocytes during Cardiac Homeostasis. Cell Rep 8(1):229-41. PubMed: 25001281MGI: J:212071
- Fischer JM; Schepers AG; Clevers H; Shibata D; Liskay RM. 2014. Occult progression by Apc-deficient intestinal crypts as a target for chemoprevention. Carcinogenesis 35(1):237-46. PubMed: 23996931MGI: J:204534
- Fletcher RB; Das D; Gadye L; Street KN; Baudhuin A; Wagner A; Cole MB; Flores Q; Choi YG; Yosef N; Purdom E; Dudoit S; Risso D; Ngai J. 2017. Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution. Cell Stem Cell 20(6):817-830.e8. PubMed: 28506465MGI: J:253895
- Frank DB; Peng T; Zepp JA; Snitow M; Vincent TL; Penkala IJ; Cui Z; Herriges MJ; Morley MP; Zhou S; Lu MM; Morrisey EE. 2016. Emergence of a Wave of Wnt Signaling that Regulates Lung Alveologenesis by Controlling Epithelial Self-Renewal and Differentiation. Cell Rep 17(9):2312-2325. PubMed: 27880906MGI: J:241237
- Furlan A; Dyachuk V; Kastriti ME; Calvo-Enrique L; Abdo H; Hadjab S; Chontorotzea T; Akkuratova N; Usoskin D; Kamenev D; Petersen J; Sunadome K; Memic F; Marklund U; Fried K; Topilko P; Lallemend F; Kharchenko PV; Ernfors P; Adameyko I. 2017. Multipotent peripheral glial cells generate neuroendocrine cells of the adrenal medulla. Science 357(6346):None. PubMed: 28684471MGI: J:244596
- Gadye L; Das D; Sanchez MA; Street K; Baudhuin A; Wagner A; Cole MB; Choi YG; Yosef N; Purdom E; Dudoit S; Risso D; Ngai J; Fletcher RB. 2017. Injury Activates Transient Olfactory Stem Cell States with Diverse Lineage Capacities. Cell Stem Cell 21(6):775-790.e9. PubMed: 29174333MGI: J:253900
- Gaertner F; Ahmad Z; Rosenberger G; Fan S; Nicolai L; Busch B; Yavuz G; Luckner M; Ishikawa-Ankerhold H; Hennel R; Benechet A; Lorenz M; Chandraratne S; Schubert I; Helmer S; Striednig B; Stark K; Janko M; Bottcher RT; Verschoor A; Leon C; Gachet C; Gudermann T; Mederos Y Schnitzler M; Pincus Z; Iannacone M; Haas R; Wanner G; Lauber K; Sixt M; Massberg S. 2017. Migrating Platelets Are Mechano-scavengers that Collect and Bundle Bacteria. Cell 171(6):1368-1382.e23. PubMed: 29195076MGI: J:250001
- Giroux V; Lento AA; Islam M; Pitarresi JR; Kharbanda A; Hamilton KE; Whelan KA; Long A; Rhoades B; Tang Q; Nakagawa H; Lengner CJ; Bass AJ; Wileyto EP; Klein-Szanto AJ; Wang TC; Rustgi AK. 2017. Long-lived keratin 15+ esophageal progenitor cells contribute to homeostasis and regeneration. J Clin Invest 127(6):2378-2391. PubMed: 28481227MGI: J:244536
- Glenn ST; Jones CA; Sexton S; LeVea CM; Caraker SM; Hajduczok G; Gross KW. 2014. Conditional deletion of p53 and Rb in the renin-expressing compartment of the pancreas leads to a highly penetrant metastatic pancreatic neuroendocrine carcinoma. Oncogene 33(50):5706-15. PubMed: 24292676MGI: J:216559
- Goldmann T; Zeller N; Raasch J; Kierdorf K; Frenzel K; Ketscher L; Basters A; Staszewski O; Brendecke SM; Spiess A; Tay TL; Kreutz C; Timmer J; Mancini GM; Blank T; Fritz G; Biber K; Lang R; Malo D; Merkler D; Heikenwalder M; Knobeloch KP; Prinz M. 2015. USP18 lack in microglia causes destructive interferonopathy of the mouse brain. EMBO J 34(12):1612-29. PubMed: 25896511MGI: J:222614
- Guo C; Eckler MJ; McKenna WL; McKinsey GL; Rubenstein JL; Chen B. 2013. Fezf2 expression identifies a multipotent progenitor for neocortical projection neurons, astrocytes, and oligodendrocytes. Neuron 80(5):1167-74. PubMed: 24314728MGI: J:207160
- Hackl MJ; Burford JL; Villanueva K; Lam L; Susztak K; Schermer B; Benzing T; Peti-Peterdi J. 2013. Tracking the fate of glomerular epithelial cells in vivo using serial multiphoton imaging in new mouse models with fluorescent lineage tags. Nat Med 19(12):1661-6. PubMed: 24270544MGI: J:206843
- Hayakawa Y; Ariyama H; Stancikova J; Sakitani K; Asfaha S; Renz BW; Dubeykovskaya ZA; Shibata W; Wang H; Westphalen CB; Chen X; Takemoto Y; Kim W; Khurana SS; Tailor Y; Nagar K; Tomita H; Hara A; Sepulveda AR; Setlik W; Gershon MD; Saha S; Ding L; Shen Z; Fox JG; Friedman RA; Konieczny SF; Worthley DL; Korinek V; Wang TC. 2015. Mist1 Expressing Gastric Stem Cells Maintain the Normal and Neoplastic Gastric Epithelium and Are Supported by a Perivascular Stem Cell Niche. Cancer Cell 28(6):800-14. PubMed: 26585400MGI: J:227252
- Hayakawa Y; Sakitani K; Konishi M; Asfaha S; Niikura R; Tomita H; Renz BW; Tailor Y; Macchini M; Middelhoff M; Jiang Z; Tanaka T; Dubeykovskaya ZA; Kim W; Chen X; Urbanska AM; Nagar K; Westphalen CB; Quante M; Lin CS; Gershon MD; Hara A; Zhao CM; Chen D; Worthley DL; Koike K; Wang TC. 2017. Nerve Growth Factor Promotes Gastric Tumorigenesis through Aberrant Cholinergic Signaling. Cancer Cell 31(1):21-34. PubMed: 27989802MGI: J:238420
- Kalfon R; Koren L; Aviram S; Schwartz O; Hai T; Aronheim A. 2017. ATF3 expression in cardiomyocytes preserves homeostasis in the heart and controls peripheral glucose tolerance. Cardiovasc Res 113(2):134-146. PubMed: 28082453MGI: J:258503
- Kaucka M; Ivashkin E; Gyllborg D; Zikmund T; Tesarova M; Kaiser J; Xie M; Petersen J; Pachnis V; Nicolis SK; Yu T; Sharpe P; Arenas E; Brismar H; Blom H; Clevers H; Suter U; Chagin AS; Fried K; Hellander A; Adameyko I. 2016. Analysis of neural crest-derived clones reveals novel aspects of facial development. Sci Adv 2(8):e1600060. PubMed: 27493992MGI: J:258183
- Kaucka M; Zikmund T; Tesarova M; Gyllborg D; Hellander A; Jaros J; Kaiser J; Petersen J; Szarowska B; Newton PT; Dyachuk V; Li L; Qian H; Johansson AS; Mishina Y; Currie JD; Tanaka EM; Erickson A; Dudley A; Brismar H; Southam P; Coen E; Chen M; Weinstein LS; Hampl A; Arenas E; Chagin AS; Fried K; Adameyko I. 2017. Oriented clonal cell dynamics enables accurate growth and shaping of vertebrate cartilage. Elife 6(None):None. PubMed: 28414273MGI: J:258122
- Kaukua N; Shahidi MK; Konstantinidou C; Dyachuk V; Kaucka M; Furlan A; An Z; Wang L; Hultman I; Ahrlund-Richter L; Blom H; Brismar H; Lopes NA; Pachnis V; Suter U; Clevers H; Thesleff I; Sharpe P; Ernfors P; Fried K; Adameyko I. 2014. Glial origin of mesenchymal stem cells in a tooth model system. Nature 513(7519):551-4. PubMed: 25079316MGI: J:217098
- Kaverina NV; Kadoya H; Eng DG; Rusiniak ME; Sequeira-Lopez ML; Gomez RA; Pippin JW; Gross KW; Peti-Peterdi J; Shankland SJ. 2017. Tracking the stochastic fate of cells of the renin lineage after podocyte depletion using multicolor reporters and intravital imaging. PLoS One 12(3):e0173891. PubMed: 28329012MGI: J:245719
- Kohler C; Nittner D; Rambow F; Radaelli E; Stanchi F; Vandamme N; Baggiolini A; Sommer L; Berx G; van den Oord JJ; Gerhardt H; Blanpain C; Marine JC. 2017. Mouse Cutaneous Melanoma Induced by Mutant BRaf Arises from Expansion and Dedifferentiation of Mature Pigmented Melanocytes. Cell Stem Cell 21(5):679-693.e6. PubMed: 29033351MGI: J:253959
- Lasrado R; Boesmans W; Kleinjung J; Pin C; Bell D; Bhaw L; McCallum S; Zong H; Luo L; Clevers H; Vanden Berghe P; Pachnis V. 2017. Lineage-dependent spatial and functional organization of the mammalian enteric nervous system. Science 356(6339):722-726. PubMed: 28522527MGI: J:243717
- Lescroart F; Chabab S; Lin X; Rulands S; Paulissen C; Rodolosse A; Auer H; Achouri Y; Dubois C; Bondue A; Simons BD; Blanpain C. 2014. Early lineage restriction in temporally distinct populations of Mesp1 progenitors during mammalian heart development. Nat Cell Biol 16(9):829-40. PubMed: 25150979MGI: J:219384
- Leushacke M; Ng A; Galle J; Loeffler M; Barker N. 2013. Lgr5(+) gastric stem cells divide symmetrically to effect epithelial homeostasis in the pylorus. Cell Rep 5(2):349-56. PubMed: 24209744MGI: J:205131
- Li J; Miao L; Shieh D; Spiotto E; Li J; Zhou B; Paul A; Schwartz RJ; Firulli AB; Singer HA; Huang G; Wu M. 2016. Single-Cell Lineage Tracing Reveals that Oriented Cell Division Contributes to Trabecular Morphogenesis and Regional Specification. Cell Rep 15(1):158-70. PubMed: 27052172MGI: J:235083
- Li L; Ginty DD. 2014. The structure and organization of lanceolate mechanosensory complexes at mouse hair follicles. Elife 3:e01901. PubMed: 24569481MGI: J:207967
- Maddipati R; Stanger BZ. 2015. Pancreatic Cancer Metastases Harbor Evidence of Polyclonality. Cancer Discov 5(10):1086-97. PubMed: 26209539MGI: J:230607
- Maruyama T; Jeong J; Sheu TJ; Hsu W. 2016. Stem cells of the suture mesenchyme in craniofacial bone development, repair and regeneration. Nat Commun 7:10526. PubMed: 26830436MGI: J:236292
- McConnell AM; Yao C; Yeckes AR; Wang Y; Selvaggio AS; Tang J; Kirsch DG; Stripp BR. 2016. p53 Regulates Progenitor Cell Quiescence and Differentiation in the Airway. Cell Rep 17(9):2173-2182. PubMed: 27880895MGI: J:241243
- Mo Y; Chen J; Humphrey DM Jr; Fodah RA; Warawa JM; Hoyle GW. 2015. Abnormal epithelial structure and chronic lung inflammation after repair of chlorine-induced airway injury. Am J Physiol Lung Cell Mol Physiol 308(2):L168-78. PubMed: 25398987MGI: J:227799
- Murphy PA; Kim TN; Huang L; Nielsen CM; Lawton MT; Adams RH; Schaffer CB; Wang RA. 2014. Constitutively active Notch4 receptor elicits brain arteriovenous malformations through enlargement of capillary-like vessels. Proc Natl Acad Sci U S A 111(50):18007-12. PubMed: 25468970MGI: J:217811
- Ollila S; Domenech-Moreno E; Laajanen K; Wong IP; Tripathi S; Pentinmikko N; Gao Y; Yan Y; Niemela EH; Wang TC; Viollet B; Leone G; Katajisto P; Vaahtomeri K; Makela TP. 2018. Stromal Lkb1 deficiency leads to gastrointestinal tumorigenesis involving the IL-11-JAK/STAT3 pathway. J Clin Invest 128(1):402-414. PubMed: 29202476MGI: J:258373
- Parmigiani E; Leto K; Rolando C; Figueres-Onate M; Lopez-Mascaraque L; Buffo A; Rossi F. 2015. Heterogeneity and Bipotency of Astroglial-Like Cerebellar Progenitors along the Interneuron and Glial Lineages. J Neurosci 35(19):7388-402. PubMed: 25972168MGI: J:221638
- Peng T; Tian Y; Boogerd CJ; Lu MM; Kadzik RS; Stewart KM; Evans SM; Morrisey EE. 2013. Coordination of heart and lung co-development by a multipotent cardiopulmonary progenitor. Nature 500(7464):589-92. PubMed: 23873040MGI: J:204743
- Pitulescu ME; Schmidt I; Giaimo BD; Antoine T; Berkenfeld F; Ferrante F; Park H; Ehling M; Biljes D; Rocha SF; Langen UH; Stehling M; Nagasawa T; Ferrara N; Borggrefe T; Adams RH. 2017. Dll4 and Notch signalling couples sprouting angiogenesis and artery formation. Nat Cell Biol 19(8):915-927. PubMed: 28714968MGI: J:247050
- Prochazka J; Prochazkova M; Du W; Spoutil F; Tureckova J; Hoch R; Shimogori T; Sedlacek R; Rubenstein JL; Wittmann T; Klein OD. 2015. Migration of Founder Epithelial Cells Drives Proper Molar Tooth Positioning and Morphogenesis. Dev Cell 35(6):713-24. PubMed: 26702830MGI: J:239990
- Ramasamy SK; Kusumbe AP; Schiller M; Zeuschner D; Bixel MG; Milia C; Gamrekelashvili J; Limbourg A; Medvinsky A; Santoro MM; Limbourg FP; Adams RH. 2016. Blood flow controls bone vascular function and osteogenesis. Nat Commun 7:13601. PubMed: 27922003MGI: J:243163
- Rios AC; Fu NY; Lindeman GJ; Visvader JE. 2014. In situ identification of bipotent stem cells in the mammary gland. Nature 506(7488):322-7. PubMed: 24463516MGI: J:206961
- Ritsma L; Ellenbroek SI; Zomer A; Snippert HJ; de Sauvage FJ; Simons BD; Clevers H; van Rheenen J. 2014. Intestinal crypt homeostasis revealed at single-stem-cell level by in vivo live imaging. Nature 507(7492):362-5. PubMed: 24531760MGI: J:208759
- Sato T; van Es JH; Snippert HJ; Stange DE; Vries RG; van den Born M; Barker N; Shroyer NF; van de Wetering M; Clevers H. 2011. Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts. Nature 469(7330):415-8. PubMed: 21113151MGI: J:168728
- Schepers AG; Snippert HJ; Stange DE; van den Born M; van Es JH; van de Wetering M; Clevers H. 2012. Lineage tracing reveals Lgr5+ stem cell activity in mouse intestinal adenomas. Science 337(6095):730-5. PubMed: 22855427MGI: J:186630
- Schutgens F; Rookmaaker MB; Blokzijl F; van Boxtel R; Vries R; Cuppen E; Verhaar MC; Clevers H. 2017. Troy/TNFRSF19 marks epithelial progenitor cells during mouse kidney development that continue to contribute to turnover in adult kidney. Proc Natl Acad Sci U S A 114(52):E11190-E11198. PubMed: 29237753MGI: J:255412
- Singh SP; LaSarge CL; An A; McAuliffe JJ; Danzer SC. 2015. Clonal Analysis of Newborn Hippocampal Dentate Granule Cell Proliferation and Development in Temporal Lobe Epilepsy. eNeuro 2(6):. PubMed: 26756038MGI: J:239973
- Snippert HJ; Schepers AG; van Es JH; Simons BD; Clevers H. 2014. Biased competition between Lgr5 intestinal stem cells driven by oncogenic mutation induces clonal expansion. EMBO Rep 15(1):62-9. PubMed: 24355609MGI: J:211965
- Suarez-Mier GB; Buckwalter MS. 2015. Glial Fibrillary Acidic Protein-Expressing Glia in the Mouse Lung. ASN Neuro 7(5):None. PubMed: 26442852MGI: J:244687
- Sun GJ; Zhou Y; Stadel RP; Moss J; Yong JH; Ito S; Kawasaki NK; Phan AT; Oh JH; Modak N; Reed RR; Toni N; Song H; Ming GL. 2015. Tangential migration of neuronal precursors of glutamatergic neurons in the adult mammalian brain. Proc Natl Acad Sci U S A 112(30):9484-9. PubMed: 26170290MGI: J:225978
- Sutherland KD; Song JY; Kwon MC; Proost N; Zevenhoven J; Berns A. 2014. Multiple cells-of-origin of mutant K-Ras-induced mouse lung adenocarcinoma. Proc Natl Acad Sci U S A 111(13):4952-7. PubMed: 24586047MGI: J:207396
- Tanaka T; Komai Y; Tokuyama Y; Yanai H; Ohe S; Okazaki K; Ueno H. 2013. Identification of stem cells that maintain and regenerate lingual keratinized epithelial cells. Nat Cell Biol 15(5):511-8. PubMed: 23563490MGI: J:197688
- Tian X; Hu T; Zhang H; He L; Huang X; Liu Q; Yu W; He L; Yang Z; Zhang Z; Zhong TP; Yang X; Yang Z; Yan Y; Baldini A; Sun Y; Lu J; Schwartz RJ; Evans SM; Gittenberger-de Groot AC; Red-Horse K; Zhou B. 2013. Subepicardial endothelial cells invade the embryonic ventricle wall to form coronary arteries. Cell Res 23(9):1075-90. PubMed: 23797856MGI: J:220957
- Uchida S; De Gaspari P; Kostin S; Jenniches K; Kilic A; Izumiya Y; Shiojima I; Grosse Kreymborg K; Renz H; Walsh K; Braun T. 2013. Sca1-derived cells are a source of myocardial renewal in the murine adult heart. Stem Cell Reports 1(5):397-410. PubMed: 24286028MGI: J:206145
- Urban N; van den Berg DL; Forget A; Andersen J; Demmers JA; Hunt C; Ayrault O; Guillemot F. 2016. Return to quiescence of mouse neural stem cells by degradation of a proactivation protein. Science 353(6296):292-5. PubMed: 27418510MGI: J:233820
- Volckaert T; Yuan T; Chao CM; Bell H; Sitaula A; Szimmtenings L; El Agha E; Chanda D; Majka S; Bellusci S; Thannickal VJ; Fassler R; De Langhe SP. 2017. Fgf10-Hippo Epithelial-Mesenchymal Crosstalk Maintains and Recruits Lung Basal Stem Cells. Dev Cell 43(1):48-59.e5. PubMed: 29017029MGI: J:245880
- Wang AB; Zhang YV; Tumbar T. 2017. Gata6 promotes hair follicle progenitor cell renewal by genome maintenance during proliferation. EMBO J 36(1):61-78. PubMed: 27908934MGI: J:239085
- Wang L; Benedito R; Bixel MG; Zeuschner D; Stehling M; Savendahl L; Haigh JJ; Snippert H; Clevers H; Breier G; Kiefer F; Adams RH. 2013. Identification of a clonally expanding haematopoietic compartment in bone marrow. EMBO J 32(2):219-30. PubMed: 23188081MGI: J:193124
- Watson JK; Rulands S; Wilkinson AC; Wuidart A; Ousset M; Van Keymeulen A; Gottgens B; Blanpain C; Simons BD; Rawlins EL. 2015. Clonal Dynamics Reveal Two Distinct Populations of Basal Cells in Slow-Turnover Airway Epithelium. Cell Rep 12(1):90-101. PubMed: 26119728MGI: J:224857
- Weng PL; Vinjamuri M; Ovitt CE. 2016. Ascl3 transcription factor marks a distinct progenitor lineage for non-neuronal support cells in the olfactory epithelium. Sci Rep 6(None):38199. PubMed: 27910949MGI: J:259982
- Worthley DL; Churchill M; Compton JT; Tailor Y; Rao M; Si Y; Levin D; Schwartz MG; Uygur A; Hayakawa Y; Gross S; Renz BW; Setlik W; Martinez AN; Chen X; Nizami S; Lee HG; Kang HP; Caldwell JM; Asfaha S; Westphalen CB; Graham T; Jin G; Nagar K; Wang H; Kheirbek MA; Kolhe A; Carpenter J; Glaire M; Nair A; Renders S; Manieri N; Muthupalani S; Fox JG; Reichert M; Giraud AS; Schwabe RF; Pradere JP; Walton K; Prakash A; Gumucio D; Rustgi AK; Stappenbeck TS; Friedman RA; Gershon MD; Sims P; Grikscheit T; Lee FY;. 2015. Gremlin 1 identifies a skeletal stem cell with bone, cartilage, and reticular stromal potential. Cell 160(1-2):269-84. PubMed: 25594183MGI: J:219328
- Wuidart A; Ousset M; Rulands S; Simons BD; Van Keymeulen A; Blanpain C. 2016. Quantitative lineage tracing strategies to resolve multipotency in tissue-specific stem cells. Genes Dev 30(11):1261-77. PubMed: 27284162MGI: J:233310
- Xie T; Liang J; Liu N; Huan C; Zhang Y; Liu W; Kumar M; Xiao R; D'Armiento J; Metzger D; Chambon P; Papaioannou VE; Stripp BR; Jiang D; Noble PW. 2016. Transcription factor TBX4 regulates myofibroblast accumulation and lung fibrosis. J Clin Invest 126(8):3063-79. PubMed: 27400124MGI: J:237128
- Xu M; Horrell J; Snitow M; Cui J; Gochnauer H; Syrett CM; Kallish S; Seykora JT; Liu F; Gaillard D; Katz JP; Kaestner KH; Levin B; Mansfield C; Douglas JE; Cowart BJ; Tordoff M; Liu F; Zhu X; Barlow LA; Rubin AI; McGrath JA; Morrisey EE; Chu EY; Millar SE. 2017. WNT10A mutation causes ectodermal dysplasia by impairing progenitor cell proliferation and KLF4-mediated differentiation. Nat Commun 8:15397. PubMed: 28589954MGI: J:242196
- Yahalom-Ronen Y; Rajchman D; Sarig R; Geiger B; Tzahor E. 2015. Reduced matrix rigidity promotes neonatal cardiomyocyte dedifferentiation, proliferation and clonal expansion. Elife 4:. PubMed: 26267307MGI: J:226637
- Yan KS; Chia LA; Li X; Ootani A; Su J; Lee JY; Su N; Luo Y; Heilshorn SC; Amieva MR; Sangiorgi E; Capecchi MR; Kuo CJ. 2012. The intestinal stem cell markers Bmi1 and Lgr5 identify two functionally distinct populations. Proc Natl Acad Sci U S A 109(2):466-71. PubMed: 22190486MGI: J:179994
- Yang H; Adam RC; Ge Y; Hua ZL; Fuchs E. 2017. Epithelial-Mesenchymal Micro-niches Govern Stem Cell Lineage Choices. Cell 169(3):483-496.e13. PubMed: 28413068MGI: J:241508
- Yoo YA; Roh M; Naseem AF; Lysy B; Desouki MM; Unno K; Abdulkadir SA. 2016. Bmi1 marks distinct castration-resistant luminal progenitor cells competent for prostate regeneration and tumour initiation. Nat Commun 7:12943. PubMed: 27703144MGI: J:242359
- Yuan D; Huang S; Berger E; Liu L; Gross N; Heinzmann F; Ringelhan M; Connor TO; Stadler M; Meister M; Weber J; Ollinger R; Simonavicius N; Reisinger F; Hartmann D; Meyer R; Reich M; Seehawer M; Leone V; Hochst B; Wohlleber D; Jors S; Prinz M; Spalding D; Protzer U; Luedde T; Terracciano L; Matter M; Longerich T; Knolle P; Ried T; Keitel V; Geisler F; Unger K; Cinnamon E; Pikarsky E; Huser N; Davis RJ; Tschaharganeh DF; Rad R; Weber A; Zender L; Haller D; Heikenwalder M. 2017. Kupffer Cell-Derived Tnf Triggers Cholangiocellular Tumorigenesis through JNK due to Chronic Mitochondrial Dysfunction and ROS. Cancer Cell 31(6):771-789.e6. PubMed: 28609656MGI: J:242561
- Zhu Z; Khan MA; Weiler M; Blaes J; Jestaedt L; Geibert M; Zou P; Gronych J; Bernhardt O; Korshunov A; Bugner V; Lichter P; Radlwimmer B; Heiland S; Bendszus M; Wick W; Liu HK. 2014. Targeting Self-Renewal in High-Grade Brain Tumors Leads to Loss of Brain Tumor Stem Cells and Prolonged Survival. Cell Stem Cell :. PubMed: 24835569MGI: J:211253
- van Es JH; Sato T; van de Wetering M; Lyubimova A; Nee AN; Gregorieff A; Sasaki N; Zeinstra L; van den Born M; Korving J; Martens AC; Barker N; van Oudenaarden A; Clevers H. 2012. Dll1+ secretory progenitor cells revert to stem cells upon crypt damage. Nat Cell Biol 14(10):1099-104. PubMed: 23000963MGI: J:193937