These knockout mice have a loxP-flanked neomycin resistance cassette replacing exons 1-2 of the Batf3) gene, abolishing gene function. This strain may be useful for studying CD8 deficiency, antigen cross-presentation, and CD8- T cell responses against viral infection and responses to tumor challenge.
Dr. Kenneth Murphy, Washington University School of Medicine
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Batf3 | basic leucine zipper transcription factor, ATF-like 3 |
These Batf3-/- knockout mice have a loxP-flanking neomycin (neo) resistance cassette replacing exons 1-2 of the basic leucine zipper transcription factor, ATF-like 3 (Batf3) gene, abolishing gene function. Mice that are homozygous for this allele are viable and fertile. Batf3 is highly expressed in CD11c+ CD8α+ conventional dendritic cells (cDCs), with low to absent expression in other immune cells.The deletion of Batf3 prevents development of splenic CD8α+ cDCs, which are important for cross-presentation during infections. Lymph nodes and thymi of Batf3-/- mice lack CD8α+ DCs, and DCs generated from Batf3-/- bone marrow (BM) and spleens are deficient in Toll-like receptor (TLR) 3-induced interleukin (IL)-12 production. These mice also lack CD103+CD11b- DCs in the lung, intestine, mesenteric lymph nodes (MLNs), dermis, and skin-draining lymph nodes. Exhibiting defects in CD8+T cell response priming, including lack of virus-specific responses, results in the inability to reject highly immunogenic syngeneic tumors. .
A targeting vector was designed to replace exons 1-2 of the basic leucine zipper transcription factor, ATF-like 3 (Batf3) gene with a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into 129S6/SvEvTac-derived MC50 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to 129SvEv females to establish a colony. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Kenneth M Murphy |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Batf3, basic leucine zipper transcription factor, ATF-like 3 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 1 |
Molecular Note | Exons 1-2 were replaced by a floxed neomycin cassette. Cre-mediated recombination removed the neo cassette. Gene inactivation was confirmed by a lack of protein detected by immunoblot analysis of Th1 CD4+ T cells. |
Mutations Made By | Dr. Kenneth Murphy, Washington University School of Medicine |
When maintaining a live colony, homozygous mice may be bred together.
When using the Batf3- mouse strain in a publication, please cite the originating article(s) and include JAX stock #013596 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Batf3<tm1Kmm> |
Frozen Mouse Embryo | 129S-Batf3<tm1Kmm>/J | $2595.00 |
Frozen Mouse Embryo | 129S-Batf3<tm1Kmm>/J | $2595.00 |
Frozen Mouse Embryo | 129S-Batf3<tm1Kmm>/J | $3373.50 |
Frozen Mouse Embryo | 129S-Batf3<tm1Kmm>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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