cellular phenotype

• abnormal dendritic cell differentiation
  • bone marrow chimera experiments indicate a lack of CD8a⁺ DCs is due to a cell-intrinsic effect of the mutant locus
  (MGI Ref ID J:141064)
  • mice lack CD8a⁺ dendritic cell subsets
  (MGI Ref ID J:141064)
  • in vitro bone marrow differentiation cultures fail to generate CD24⁺ Sirp-alpha^- DCs that are similar to CD8a⁺ DC
  (MGI Ref ID J:141064)

immune system phenotype

• abnormal dendritic cell differentiation
  • bone marrow chimera experiments indicate a lack of CD8a⁺ DCs is due to a cell-intrinsic effect of the mutant locus
  (MGI Ref ID J:141064)
  • mice lack CD8a⁺ dendritic cell subsets
  (MGI Ref ID J:141064)
  • in vitro bone marrow differentiation cultures fail to generate CD24⁺ Sirp-alpha^- DCs that are similar to CD8a⁺ DC
  (MGI Ref ID J:141064)

• abnormal dendritic cell physiology
  • conventional DCs fail to secrete IL-12 after TL3 stimulation but have normal responses to TLR4 and TLR9 stimulation
  (MGI Ref ID J:141064)

• decreased myeloid dendritic cell number
  • mice have a loss of CD11c^hiCD11b^dull DCs and CD11c^hi CD8a⁺CD24⁺ DCs
  (MGI Ref ID J:141064)
  • mice have normal populations of CD4⁺ and CD8a-CD4- conventional DC subsets
  (MGI Ref ID J:141064)
  • mice lack splenic CD11c^hiCD8a⁺DEC205⁺ dendritic cells
  (MGI Ref ID J:141064)
  • lymph nodes and thymi lack CD8a⁺ DC subsets
  (MGI Ref ID J:141064)
  • CD103-⁺DEC205⁺CD8a^-CD11b^lo/- dermal DC numbers are reduced 5-fold in draining lymph nodes of the skin
  (MGI Ref ID J:141064)

• abnormal cytotoxic T cell physiology
  • CD8⁺ T cells from mice infected with West Nile virus (WNV) have defective IFN-gamma secretion when cultured with WNV-peptide
  (MGI Ref ID J:141064)
  • infiltrating CD8⁺ T cells fail to infiltrate transplanted syngenic tumors
  (MGI Ref ID J:141064)
  • bone marrow chimera studies demonstrate that defective cytolytic activity is not intrinsic to the CD8⁺ T cells but rather defective dendritic cell function
  (MGI Ref ID J:141064)

• decreased cytotoxic T cell cytolysis
  • CD8⁺ T cells from mice infected with West Nile virus (WNV) have defective killing of WNV peptide-loaded target cells
  (MGI Ref ID J:141064)

• decreased memory T cell number
  • mice fail to develop West Nile Virus (WNV)-specific memory CD8⁺ T cells after infection with the virus
  (MGI Ref ID J:141064)
  • the number of CD8⁺CD44^hiCD62L^lowcells memory cells is about half-that of wild-type mice before infection and is five-fold less after infection
  (MGI Ref ID J:141064)

• abnormal dendritic cell antigen presentation
  • conventional DCs are unable to cross-present (i.e. take up protein, process into peptide and present) ovalbumin antigen to ova-specific CD8⁺ T cells
  (MGI Ref ID J:141064)

• abnormal immune system physiology
  • transplanted syngenic fibrosarcomas grow in size instead of being reapidly rejected as happens in control mice
  (MGI Ref ID J:141064)

hematopoietic system phenotype

• abnormal dendritic cell differentiation
  • bone marrow chimera experiments indicate a lack of CD8a⁺ DCs is due to a cell-intrinsic effect of the mutant locus
  (MGI Ref ID J:141064)
  • mice lack CD8a⁺ dendritic cell subsets
  (MGI Ref ID J:141064)
  • in vitro bone marrow differentiation cultures fail to generate CD24⁺ Sirp-alpha^- DCs that are similar to CD8a⁺ DC
  (MGI Ref ID J:141064)

• abnormal cytotoxic T cell physiology
  • infiltrating CD8⁺ T cells fail to infiltrate transplanted syngenic tumors
  (MGI Ref ID J:141064)
  • CD8⁺ T cells from mice infected with West Nile virus (WNV) have defective IFN-gamma secretion when cultured with WNV-peptide
  (MGI Ref ID J:141064)
  • bone marrow chimera studies demonstrate that defective cytolytic activity is not intrinsic to the CD8⁺ T cells but rather defective dendritic cell function
  (MGI Ref ID J:141064)

• decreased myeloid dendritic cell number
  • mice lack splenic CD11c^hiCD8a⁺DEC205⁺ dendritic cells
  (MGI Ref ID J:141064)
  • mice have a loss of CD11c^hiCD11b^dull DCs and CD11c^hi CD8a⁺CD24⁺ DCs
  (MGI Ref ID J:141064)
  • lymph nodes and thymi lack CD8a⁺ DC subsets
  (MGI Ref ID J:141064)
  • CD103-⁺DEC205⁺CD8a^-CD11b^lo/- dermal DC numbers are reduced 5-fold in draining lymph nodes of the skin
  (MGI Ref ID J:141064)
  • mice have normal populations of CD4⁺ and CD8a-CD4- conventional DC subsets
  (MGI Ref ID J:141064)

• decreased cytotoxic T cell cytolysis
  • CD8⁺ T cells from mice infected with West Nile virus (WNV) have defective killing of WNV peptide-loaded target cells
  (MGI Ref ID J:141064)

• decreased memory T cell number
  • mice fail to develop West Nile Virus (WNV)-specific memory CD8⁺ T cells after infection with the virus
  (MGI Ref ID J:141064)
  • the number of CD8⁺CD44^hiCD62L^lowcells memory cells is about half-that of wild-type mice before infection and is five-fold less after infection
  (MGI Ref ID J:141064)

hematopoietic system phenotype

• abnormal cytotoxic T cell physiology
  • reduced cytotoxic T cell response to anti-Clec9a coupled antigen
  (MGI Ref ID J:181366)

immune system phenotype

• abnormal cytotoxic T cell physiology
  • reduced cytotoxic T cell response to anti-Clec9a coupled antigen
  (MGI Ref ID J:181366)

mammalian phenotype

• immune system phenotype
  • Normal - homozygotes retain an enhanced antibody response to anti-Clec9a coupled antigen
  (MGI Ref ID J:181366)