129S-Batf3^tm1Kmm/J

Stock number: 013596
Product name: Batf3^-
Research areas: Immunology, Inflammation and Autoimmunity Research, Virology Research

Description

These knockout mice have a loxP-flanked neomycin resistance cassette replacing exons 1-2 of the Batf3) gene, abolishing gene function. This strain may be useful for studying CD8 deficiency, antigen cross-presentation, and CD8^- T cell responses against viral infection and responses to tumor challenge.

Detailed description

These Batf3^-/- knockout mice have a loxP-flanking neomycin (neo) resistance cassette replacing exons 1-2 of the basic leucine zipper transcription factor, ATF-like 3 (Batf3) gene, abolishing gene function. Mice that are homozygous for this allele are viable and fertile. Batf3 is highly expressed in CD11c⁺ CD8α⁺ conventional dendritic cells (cDCs), with low to absent expression in other immune cells.The deletion of Batf3 prevents development of splenic CD8α⁺ cDCs, which are important for cross-presentation during infections. Lymph nodes and thymi of Batf3^-/- mice lack CD8α⁺ DCs, and DCs generated from Batf3^-/- bone marrow (BM) and spleens are deficient in Toll-like receptor (TLR) 3-induced interleukin (IL)-12 production. These mice also lack CD103⁺CD11b^- DCs in the lung, intestine, mesenteric lymph nodes (MLNs), dermis, and skin-draining lymph nodes. Exhibiting defects in CD8⁺T cell response priming, including lack of virus-specific responses, results in the inability to reject highly immunogenic syngeneic tumors. .

Development

A targeting vector was designed to replace exons 1-2 of the basic leucine zipper transcription factor, ATF-like 3 (Batf3) gene with a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into 129S6/SvEvTac-derived MC50 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to 129SvEv females to establish a colony. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation to establish the colony.

Allele

Symbol: Batf3^tm1Kmm
Name: targeted mutation 1, Kenneth M Murphy
MGI accession ID: MGI:3817232
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Batf3
Marker name: basic leucine zipper transcription factor, ATF-like 3
Chromosome: 1
Strain of origin: 129S6/SvEvTac
Molecular note: Exons 1-2 were replaced by a floxed neomycin cassette. Cre-mediated recombination removed the neo cassette. Gene inactivation was confirmed by a lack of protein detected by immunoblot analysis of Th1 CD4⁺ T cells.